Does Gender Difference Effect Radiation-Induced Lung Toxicity? An Experimental Study by Genetic and Histopathological Predictors

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dc.authoridCosar, Rusen/0000-0002-7953-8887
dc.authoridPARLAR, SULE/0000-0001-9422-853X
dc.contributor.authorCosar, Rusen
dc.contributor.authorOzen, Alaattin
dc.contributor.authorTastekin, Ebru
dc.contributor.authorSut, Necdet
dc.contributor.authorCakina, Suat
dc.contributor.authorDemir, Selma
dc.contributor.authorParlar, Sule
dc.date.accessioned2025-01-27T20:41:01Z
dc.date.available2025-01-27T20:41:01Z
dc.date.issued2022
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractSeveral studies have reported differences in radiation toxicity between the sexes, but these differences have not been tested with respect to histopathology and genes. This animal study aimed to show an association between histopathological findings of radiation-induced lung toxicity and the genes ATM, SOD2, TGF-beta 1, XRCC1, XRCC3 and HHR2. In all, 120 animals were randomly divided into 2 control groups (male and female) and experimental groups comprising fifteen rats stratified by sex, radiotherapy (0 Gy vs. 10 Gy), and time to sacrifice (6, 12, and 24 weeks postirradiation). Histopathological evaluations for lung injury, namely, intra-alveolar edema, alveolar neutrophils, intra-alveolar erythrocytes, activated macrophages, intra-alveolar fibrosis, hyaline arteriosclerosis, and collapse were performed under a light microscope using a grid system; the evaluations were semi quantitatively scored. Then, the alveolar wall thickness was measured. Real-time quantitative reverse transcription PCR (RT-qPCR) was used to determine gene expression differences in ATM, TGF-beta 1, XRCC1, XRCC3, SOD2 and HHR2L among the groups. Histopathological data showed that radiation-induced acute, subacute, and chronic lung toxicity were worse in male rats. The expression levels of the evaluated genes were significantly higher in females than males in the control group, but this difference was lost over time after radiotherapy. Less toxicity in females may be attributable to the fact that the expression of the evaluated genes was higher in normal lung tissue in females than in males and the changes in gene expression patterns in the postradiotherapy period played a protective role in females. Additional data related to pulmonary function, lung weights, imaging, or outcomes are needed to support this data that is based on histopathology alone. (C) 2022 by Radiation Research Society
dc.identifier.doi10.1667/RADE-21-00075.1
dc.identifier.endpage288
dc.identifier.issn0033-7587
dc.identifier.issn1938-5404
dc.identifier.issue3
dc.identifier.pmid34735567
dc.identifier.scopus2-s2.0-85125852006
dc.identifier.scopusqualityQ2
dc.identifier.startpage280
dc.identifier.urihttps://doi.org/10.1667/RADE-21-00075.1
dc.identifier.urihttps://hdl.handle.net/20.500.12428/23969
dc.identifier.volume197
dc.identifier.wosWOS:000763750500008
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherRadiation Research Soc
dc.relation.ispartofRadiation Research
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectIonizing-Radiation
dc.subjectRadiotherapy
dc.subjectCancer
dc.subjectRadiogenomics
dc.subjectTherapy
dc.subjectPolymorphisms
dc.subjectRadiobiology
dc.subjectPneumonitis
dc.subjectExpression
dc.subjectOncology
dc.titleDoes Gender Difference Effect Radiation-Induced Lung Toxicity? An Experimental Study by Genetic and Histopathological Predictors
dc.typeArticle

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