Development of an interference-minimized amperometric-FIA glucose biosensor at a pyrocatechol violet/glucose dehydrogenase-modified graphite pencil electrode

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dc.authoridDilgin, Yusuf/0000-0002-2980-6871
dc.authoridKarakaya, Serkan/0000-0002-6401-3295
dc.contributor.authorGuenes, Mehmet
dc.contributor.authorKarakaya, Serkan
dc.contributor.authorDilgin, Yusuf
dc.date.accessioned2025-01-27T20:31:21Z
dc.date.available2025-01-27T20:31:21Z
dc.date.issued2020
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractA modified disposable electrode formed by immobilization of glucose dehydrogenase (DHG) onto pyrocatechol violet (Pcv)-modified graphite pencil electrode (GPE) was proposed for the flow injection (FI) amperometric glucose biosensor. Cyclic voltammetric experiments show that Pcv illustrates a good electrocatalytic effect towards the oxidation of enzymatically produced NADH. Although electrocatalytic oxidation of enzymatically generated NADH at the DHG/Poly-Pcv/GPE was successfully performed at + 250 mV in FIA system, some molecules such as ascorbic acid (AA), dopamine (DA) and uric acid (UA) give a significant positive interference due to their oxidation at this working potential. To overcome these interferences, an injector filled with a pre-oxidant, sodium bismuthate (NaBiO3), was used in the FI amperometric glucose biosensor at the DHG/Poly-Pcv/GPE. Results showed that the interferences of these molecules were significantly minimized, because they were oxidized by NaBiO3 in the injector before reaching the electrode surface in the flow cell. The constructed biosensor showed that a linear calibration curve was obtained in the range between 5.0 mu M and 500 mu M glucose with a detection limit of 1.2 mu M. This proposed glucose biosensor including elimination of interferences of some oxidizable species was successfully applied to the real and artificial samples.
dc.description.sponsorshipTUBITAK [115Z235]
dc.description.sponsorshipThe author thanks TUBITAK for financial support (Project number: 115Z235). A large part of this study is from the PhD thesis of Mehmet Gunes.
dc.identifier.doi10.1007/s11696-019-01036-w
dc.identifier.endpage1936
dc.identifier.issn2585-7290
dc.identifier.issn1336-9075
dc.identifier.issue6
dc.identifier.scopus2-s2.0-85077592446
dc.identifier.scopusqualityQ2
dc.identifier.startpage1923
dc.identifier.urihttps://doi.org/10.1007/s11696-019-01036-w
dc.identifier.urihttps://hdl.handle.net/20.500.12428/23112
dc.identifier.volume74
dc.identifier.wosWOS:000505358200003
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherSpringer International Publishing Ag
dc.relation.ispartofChemical Papers
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectGraphite pencil electrode
dc.subjectPyrocatechol violet
dc.subjectFlow injection analysis
dc.subjectAmperometric biosensor
dc.subjectGlucose
dc.subjectPre-oxidant
dc.titleDevelopment of an interference-minimized amperometric-FIA glucose biosensor at a pyrocatechol violet/glucose dehydrogenase-modified graphite pencil electrode
dc.typeArticle

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