Supercritical CO2 processing of a chitosan-based scaffold: Can implantation of osteoblastic cells be enhanced?

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dc.authoridOzdemir Kaynak, Elif/0000-0001-6667-8007
dc.authoridSendemir, Aylin/0000-0003-1818-6651
dc.authoridYesil-Celiktas, Ozlem/0000-0003-4509-2212
dc.contributor.authorOzdemir, Elif
dc.contributor.authorSendemir-Urkmez, Aylin
dc.contributor.authorYesil-Celiktas, Ozlem
dc.date.accessioned2025-01-27T20:31:22Z
dc.date.available2025-01-27T20:31:22Z
dc.date.issued2013
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractThe effects of fabrication techniques were investigated for preparation of a chitosan based scaffold. A two-step process was used: fabrication of hydrogel which was subsequently processed either with supercritical CO2 (SC-CO2) or lyophilization. Various pressures from subcritical to supercritical (70, 160, 250 bar), temperatures (35, 45, 55 degrees C) and durations (2-3 h) were applied in order to elicit the optimum process parameters yielding the highest porosity which were determined as 250 bar, 45 degrees C, 2 h of processing at 5 g/min CO2 flow rate yielding a porosity of 87.03% which was similar to lyophilization (88.68%) achieved at 55 degrees C for 48 h. When osteosarcoma cells possessing cellular features of osteoblasts were seeded, SC-CO2 dried scaffold proved to be a more ideal support for cell attachment owing to the presence of both nano and micropores, thereby providing a more efficient and rapid alternative for tissue engineering applications. (c) 2013 Elsevier B.V. All rights reserved.
dc.identifier.doi10.1016/j.supflu.2012.12.031
dc.identifier.endpage127
dc.identifier.issn0896-8446
dc.identifier.issn1872-8162
dc.identifier.scopus2-s2.0-84873865345
dc.identifier.scopusqualityQ1
dc.identifier.startpage120
dc.identifier.urihttps://doi.org/10.1016/j.supflu.2012.12.031
dc.identifier.urihttps://hdl.handle.net/20.500.12428/23121
dc.identifier.volume75
dc.identifier.wosWOS:000315756900015
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherElsevier Science Bv
dc.relation.ispartofJournal of Supercritical Fluids
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectTissue engineering
dc.subjectChitosan scaffold
dc.subjectSupercritical CO2 drying
dc.subjectPorosity
dc.subjectOsteoblastic cell
dc.titleSupercritical CO2 processing of a chitosan-based scaffold: Can implantation of osteoblastic cells be enhanced?
dc.typeArticle

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