Expression levels of KCNQ1 and KCNQ3 genes in experimental epilepsy model

Basit Öğe Kaydı
dc.authoridOztopuz, Ozlem/0000-0002-1373-6311
dc.contributor.authorCoskun, Ozlem
dc.contributor.authorOztopuz, Ozlem
dc.contributor.authorCokyaman, Turgay
dc.date.accessioned2025-01-27T20:41:10Z
dc.date.available2025-01-27T20:41:10Z
dc.date.issued2020
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractPurpose: Status epilepticus (SE) is a highly common neurological disease in children, with recurrent generalized convulsions for more than 30 minutes, and when not controlled, neuronal damage occurs in the brain. The aim of this study was to evaluate the changes in KCNQ1 and KCNQ3 gene expression levels in the acute period after SE. Materials and Methods: In rats, an experimental SE model was created with Li-Pc. Study; female Wistar albino [250-350 g, 21 (n=7)] rats were used in 3 groups as SE, control and sham groups. The total RNA obtained from brain tissues was converted to cDNA, and gene expression levels of KCNQ1 and KCNQ3 genes were assessed by quantitative real-time PCR (qRT-PCR) method. Results: Statistically in SE group; compared to the control and sham groups, a significant difference was observed in the gene expression levels of the KCNQ1 and KCNQ3 ion channels. The KCNQ1 and KCNQ3 gene expression levels of the experimental group was found higher than the other groups. Conclusion: Determining the changes in mRNA expression levels of genes encoding K+ ion channels will help to better understand the pathological mechanisms that occur during epilepsy. In the SE experimental model created, it is believed that an increase in mRNA expression of KCNQ1 and KCNQ3 will lead to drug therapy studies planned for the future.
dc.identifier.doi10.17826/cumj.741811
dc.identifier.endpage1586
dc.identifier.issn2602-3032
dc.identifier.issn2602-3040
dc.identifier.issue4
dc.identifier.startpage1580
dc.identifier.trdizinid1114377
dc.identifier.urihttps://doi.org/10.17826/cumj.741811
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1114377
dc.identifier.urihttps://hdl.handle.net/20.500.12428/24049
dc.identifier.volume45
dc.identifier.wosWOS:000609003200037
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.publisherCukurova Univ, Fac Medicine
dc.relation.ispartofCukurova Medical Journal
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectEpilepsy brain
dc.subjectLi-pilocarpine
dc.subjectstatus epilepticus (SE)
dc.subjectKCNQ1
dc.subjectKCNQ3
dc.subjectrat
dc.titleExpression levels of KCNQ1 and KCNQ3 genes in experimental epilepsy model
dc.typeArticle

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
TR-Dizin İndeksli Yayınlar Koleksiyonu