Enhanced enzymatic activity and stability by in situ entrapment of α Glucosidase within super porous p(HEMA) cryogels during synthesis

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dc.authoridDemirci, Şahin / 0000-0001-7083-1481
dc.authoridŞahiner, Mehtap / 0000-0001-8666-7954
dc.authoridYılmaz, Selehattin / 0000-0003-4607-3523
dc.authoridŞahiner, Nurettin / 0000-0003-0120-530X
dc.contributor.authorDemirci, Şahin
dc.contributor.authorŞahiner, Mehtap
dc.contributor.authorYılmaz, Selehattin
dc.contributor.authorKaradağ, Erdener
dc.contributor.authorŞahiner, Nurettin
dc.date.accessioned2025-01-27T18:53:24Z
dc.date.available2025-01-27T18:53:24Z
dc.date.issued2020
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractHere, poly(2-hydroxyethyl methacrylate) (p(HEMA)) cryogel were prepared in the presence 0.48, 0.96, and 1.92 mL of α-Glucosidase enzyme (0.06 Units/mL) solutions to obtain enzyme entrapped superporous p(HEMA) cryogels, donated as α-Glucosidase@p(HEMA)-1, α-Glucosidase@p(HEMA)-2, and α-Glucosidase@p(HEMA)-3, respectively. The enzyme entrapped p(HEMA) cryogels revealed no interruption for hemolysis and coagulation of blood rendering viable biomedical application in blood contacting applications. The α-Glucosidase@p(HEMA)-1 was found to preserve its’ activity% 92.3 ± 1.4 % and higher activity% against free α-Glucosidase enzymes in 15–60℃ temperature, and 4–9 pH range. The Km and Vmax values of α-Glucosidase@p(HEMA)-1 cryogel was calculated as 3.22 mM, and 0.0048 mM/min, respectively versus 1.97 mM, and 0.0032 mM/min, for free enzymes. The α-Glucosidase@p(HEMA)-1 cryogel was found to maintained enzymatic activity more than 50 % after 10 consecutive uses, and also preserved their activity more than 50 % after 10 days of storage at 25 ℃, whereas free α-Glucosidase enzyme maintained only 1.9 ± 0.9 % activity under the same conditions.
dc.description.sponsorshipCouncil of Higher Education System, (YOK-100/2000)
dc.identifier.doi10.1016/j.btre.2020.e00534
dc.identifier.issn2215-017X
dc.identifier.scopus2-s2.0-85091638865
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1016/j.btre.2020.e00534
dc.identifier.urihttps://hdl.handle.net/20.500.12428/12706
dc.identifier.volume28
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherElsevier B.V.
dc.relation.ispartofBiotechnology Reports
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_Scopus_20250125
dc.subjectEnzymatic reaction; Enzyme; Enzyme immobilization/entrapment; Super porous cryogel; ?-Glucosidase
dc.titleEnhanced enzymatic activity and stability by in situ entrapment of α Glucosidase within super porous p(HEMA) cryogels during synthesis
dc.typeArticle

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