A disposable and ultrasensitive ITO based biosensor modified by 6-phosphonohexanoic acid for electrochemical sensing of IL-1? in human serum and saliva

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dc.contributor.authorAydin, Elif Burcu
dc.contributor.authorSezgintürk, Mustafa Kemal
dc.date.accessioned2025-01-27T20:57:44Z
dc.date.available2025-01-27T20:57:44Z
dc.date.issued2018
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractIn this study, we constructed a new and sensitive ITO based electrochemical immunosensor for detection of interleukin 1 beta(IL-1 beta), a cancer biomarker found in serum and saliva. 6-phosphonohexanoic acid (PHA) was used as a biomolecule immobilization matrix for the first time. Anti-IL-1 beta antibody was utilized as a biorecognition molecule that immobilized onto carboxyl groups of 6-phosphohexanoic acid (PHA) via amide bond. Selective interaction between anti-IL-1 beta antibodies and IL-1 beta antigens was investigated by Electrochemical Impedance Spectroscopy (EIS), Cyclic Voltammetry (CV) and Single Frequency Impedance (SFI) methods. The surface characterization of the immunosensor was performed by fourier transform infrared spectroscopy (FTIR), raman spectroscopy, scanning electron microscopy (SEM), SEM-energy dispersive X-ray spectroscopy (EDX) and atomic force microscopy (AFM) in order to illustrate individual steps of biosensor construction. Under the optimized experimental conditions, the change in impedance was proportional to IL-1 beta concentrations in the range of 0.025-3 pg/mL (R-2 = 0.99) with detection limit of 7.5 fg/mL. The reproducibility, repeatability, stability, and specificity of the developed immunosensor were analyzed. In addition, the developed immunosensor was successfully utilized for the determination of IL-1 beta in serum and saliva samples by using the standard addition method with recoveries of 96.7-105.4%. This immunosensor was applicable for the requirements of routine analysis with respect to performance, functionality and cost. (C) 2018 Elsevier B.V. All rights reserved.
dc.description.sponsorshipTUBITAK (The Scientific and Technological Research Council of Turkey) [113 Z 678]
dc.description.sponsorshipThe financial support from TUBITAK (The Scientific and Technological Research Council of Turkey, Project number: 113 Z 678) is gratefully acknowledged.
dc.identifier.doi10.1016/j.aca.2018.07.055
dc.identifier.endpage50
dc.identifier.issn0003-2670
dc.identifier.issn1873-4324
dc.identifier.pmid30322551
dc.identifier.scopus2-s2.0-85050743993
dc.identifier.scopusqualityQ1
dc.identifier.startpage41
dc.identifier.urihttps://doi.org/10.1016/j.aca.2018.07.055
dc.identifier.urihttps://hdl.handle.net/20.500.12428/26469
dc.identifier.volume1039
dc.identifier.wosWOS:000447155200004
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier Science Bv
dc.relation.ispartofAnalytica Chimica Acta
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subject6-Phosphonohexanoic acid
dc.subjectInterlekin-1 beta
dc.subjectIndium tin oxide
dc.subjectElectrochemical impedance spectroscopy
dc.subjectSingle frequency impedance
dc.titleA disposable and ultrasensitive ITO based biosensor modified by 6-phosphonohexanoic acid for electrochemical sensing of IL-1? in human serum and saliva
dc.typeArticle

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