Reduced colonic mucin degradation in breastfed infants colonized by Bifidobacteriumlongum subsp. infantis EVC001

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dc.authoridFrese, Steven/0000-0003-2053-5830
dc.authoridKARAV, SERCAN/0000-0003-4056-1673
dc.contributor.authorKarav, Sercan
dc.contributor.authorCasaburi, Giorgio
dc.contributor.authorFrese, Steven A.
dc.date.accessioned2025-01-27T20:39:19Z
dc.date.available2025-01-27T20:39:19Z
dc.date.issued2018
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractMucin glycoproteins play an important role in protecting the gut epithelium by keeping gut microbes from direct contact with the gut epithelium while allowing for diffusion of small molecules from the lumen to the epithelium. The mucin glycocalyx can be degraded by gut bacteria such as Bacteroides and Akkermansia, but other bacteria, such as Bifidobacteriumlongum subsp. Infantis, cannot consume mucin glycans. Untargeted mass spectrometry profiles were compared to microbiome profiles to assess how different gut microbiomes affect colonic mucin degradation. Samples obtained from nine infants colonized by Bifidobacteriuminfantis EVC001 and from 10 infants colonized by higher levels of mucolytic taxa (controls), including Bacteroides, were compared. Previously performed untargeted nano-high-performance liquid chromatography-chip/time-of-flight mass spectrometry was used to detect and quantify glycans originating from colonic mucin. Colonic mucin-derived O-glycans from control infants composed 37.68% (+/- 3.14% SD) of the total glycan structure pool, whereas colonic mucin-derived O-glycans made up of only 1.78% (+/- 0.038% SD) of the total in B.infantis EVC001 samples. The relative abundance of these colonic mucin-derived O-glycans in the total glycan pool was higher among control, 26.98% (+/- 8.48% SD), relative to B.infantis-colonized infants, 1.68% (+/- 1.12% SD). Key taxa, such as Bacteroidaceae, were significantly and positively correlated with the abundance of these structures, while Bifidobacteriaceae were significantly and negatively associated with these structures. These results suggest that colonization of infants by B.infantis may diminish colonic glycan degradation and help maintain barrier function in the gastrointestinal tract of infants.
dc.identifier.doi10.1002/2211-5463.12516
dc.identifier.endpage1657
dc.identifier.issn2211-5463
dc.identifier.issue10
dc.identifier.pmid30338216
dc.identifier.scopus2-s2.0-85053480025
dc.identifier.scopusqualityQ2
dc.identifier.startpage1649
dc.identifier.urihttps://doi.org/10.1002/2211-5463.12516
dc.identifier.urihttps://hdl.handle.net/20.500.12428/23921
dc.identifier.volume8
dc.identifier.wosWOS:000446166100008
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherWiley
dc.relation.ispartofFebs Open Bio
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WoS_20250125
dc.subjectBacteroides
dc.subjectBifidobacteriuminfantis
dc.subjectglycome
dc.subjectmucin
dc.subjectmucinlike glycans
dc.titleReduced colonic mucin degradation in breastfed infants colonized by Bifidobacteriumlongum subsp. infantis EVC001
dc.typeArticle

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