11-(Triethoxysilyl) Undecanal Agent-Based Biosensor System Using Disposable ITO- PET Electrode for Tumour Necrosis Factor-Alpha Detection

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dc.contributor.authorDemirbakan, Burçak
dc.date.accessioned2025-01-27T19:15:22Z
dc.date.available2025-01-27T19:15:22Z
dc.date.issued2023
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractIn this study, a label-free electrochemical biosensor system based on a disposable indium tin oxide polyethylene terephthalate (ITO-PET) electrode modified with the 11-(triethoxysilyl) undecanal (11-TESU) agent was developed for the detection of tumour necrosis factor-alpha (TNF-?) in serum. The developed biosensor was observed with electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV) techniques, square wave voltammetry (SWV) and single frequency impedance (SFI) technique which is utilized for the specific interaction between anti-TNF-? and TNF-? antigen. In addition, scanning electron microscopy was used to look at how the morphology of each ITO-PET surface changed (SEM). All parameters such as 11-TESU concentration, anti-TNF-? concentration and anti-TNF-? incubation time, were optimized. The biosensor system was characterized by measuring its linear determination range, repeatability, reproducibility, reusability, storage stability, and surface coverage. The TNF-?electrochemical biosensor showed high levels of repeatability and reproducibility as well as a large dynamic range of detection (from 0.03 pg mL-1 to 3 pg mL-1). The LOD and LOQ for the biosensor were extremely low at 1x10-4 pg mL-1 and 5x10-4 pg mL-1, respectively. It was applied to real samples to determine whether the proposed biosensor would be useful in clinical settings.
dc.identifier.doi10.15671/hjbc.1240299
dc.identifier.endpage281
dc.identifier.issn1303-5002
dc.identifier.issn2687-475X
dc.identifier.issue3
dc.identifier.startpage271
dc.identifier.trdizinid1200446
dc.identifier.urihttps://doi.org/10.15671/hjbc.1240299
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1200446
dc.identifier.urihttps://hdl.handle.net/20.500.12428/14803
dc.identifier.volume51
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.relation.ispartofHacettepe Journal of Biology and Chemistry
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_TRD_20250125
dc.subjectMikroskopi
dc.subjectBiyoloji
dc.subjectTıbbi İnformatik
dc.subjectGörüntüleme Bilimi ve Fotoğraf Teknolojisi
dc.subjectRobotik
dc.subjectNörolojik Bilimler
dc.subjectKimya
dc.subjectTıbbi
dc.subjectOnkoloji
dc.title11-(Triethoxysilyl) Undecanal Agent-Based Biosensor System Using Disposable ITO- PET Electrode for Tumour Necrosis Factor-Alpha Detection
dc.typeArticle

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