A novel electrochemical immunosensor based on ITO modified by carboxyl-ended silane agent for ultrasensitive detection of MAGE-1 in human serum

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dc.authoridAYDIN, Elif Burcu/0000-0002-1982-4128
dc.contributor.authorGundogdu, Ash
dc.contributor.authorAydin, Elif Burcu
dc.contributor.authorSezgintürk, Mustafa Kemal
dc.date.accessioned2025-01-27T20:29:01Z
dc.date.available2025-01-27T20:29:01Z
dc.date.issued2017
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractA new, low-cost electrochemical immunosensor was developed for rapid detection of Melanoma associated antigen 1 (MAGE-1), a cancer biomarker. The fabrication procedure of immunosensor was based on the covalent immobilization of anti-MAGE-1, biorecognition molecule, on ITO electrode by carboxyethylsilanetriol (CTES) monolayer. The biosensing MAGE-1 antigen was monitored by using electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) technique. Apart from these techniques, single frequency impedance (SFI) was used for investigation of antibody-antigen interactions. Scanning electron microscopy (SEM), fourier transform infrared spectroscopy (FTIR), atomic force microscopy (AFM) were utilized for characterization of the proposed biosensor. To fabricate highly sensitive, good stability immunosensor, some parameters were optimized. Under optimal conditions, the developed electrochemical immunosensor for MAGE-1 exhibited a dynamic range of 4 fg/mL and 200 fg/mL with a low detection limit of 130 fg/mL. It had acceptable repeatability (5.05%, n = 20) and good storage stability (3.58% loss after 10 weeks). Moreover, this electrochemical immunosensor has been successfully applied to the determination of MAGE-1 in human serum samples. (C) 2017 Elsevier Inc. All rights reserved.
dc.description.sponsorshipTUBITAK (The Scientific and Technological Research Council of Turkey) [113 Z 678]
dc.description.sponsorshipThe financial support from TUBITAK (The Scientific and Technological Research Council of Turkey, Project number: 113 Z 678) is gratefully acknowledged.
dc.identifier.doi10.1016/j.ab.2017.08.018
dc.identifier.endpage92
dc.identifier.issn0003-2697
dc.identifier.issn1096-0309
dc.identifier.pmid28916435
dc.identifier.scopus2-s2.0-85029372134
dc.identifier.scopusqualityQ2
dc.identifier.startpage84
dc.identifier.urihttps://doi.org/10.1016/j.ab.2017.08.018
dc.identifier.urihttps://hdl.handle.net/20.500.12428/22778
dc.identifier.volume537
dc.identifier.wosWOS:000417116700016
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherAcademic Press Inc Elsevier Science
dc.relation.ispartofAnalytical Biochemistry
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectMelanoma-associated antigen 1
dc.subjectElectrochemical immunosensor
dc.subjectIndium tin oxide
dc.subjectCarboxyethylsilanetriol
dc.titleA novel electrochemical immunosensor based on ITO modified by carboxyl-ended silane agent for ultrasensitive detection of MAGE-1 in human serum
dc.typeArticle

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