Construction of succinimide group substituted polythiophene polymer functionalized sensing platform for ultrasensitive detection of KLK 4 cancer biomarker

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dc.authoridAYDIN, MUHAMMET/0000-0002-8716-9970
dc.authoridAYDIN, Elif Burcu/0000-0002-1982-4128
dc.contributor.authorAydin, Elif Burcu
dc.contributor.authorAydin, Muhammet
dc.contributor.authorSezgintürk, Mustafa Kemal
dc.date.accessioned2025-01-27T20:27:24Z
dc.date.available2025-01-27T20:27:24Z
dc.date.issued2020
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractA novel impedimetric immunosensor based on conjugated succinimide group substituted polythiophene polymer (SucS-PThi) modified indium tin oxide (ITO) electrode was fabricated for ultra-sensitive and ultra-selective determination of kallikrein 4 (KLK 4) antigen in diluted human serum. The basic reason for the usage of SucS-PThi polymer was that its rich effective functional groups and biocompatibility. KLK 4, an important prostate biomarker was the target analyte and anti-KLK 4 antibodies immobilized electrode was the diagnostic tool for KLK 4 antigen detection. The fabricated biosensor was characterized by employing electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV), scanning electron microscopy (SEM) and energy dispersive X-ray (EDX), fourier transform-infrared (FTIR) and Raman spectroscopy. Under optimized conditions, a linear concentration range from 0.04 pg/mL to 30 pg/mL and a low detection limit (LOD) of 12.2 fg/mL were obtained and the LOD was lower than of most of the existing techniques, illustrating its feasibility for practical application. Moreover, the developed sensing system combined the outstanding properties in terms of selectivity, sensitivity, repeatability, reproducibility and reusability, without requiring for labor - intensive labeling stages. In addition, it was successfully utilized for accurate quantification of KLK 4 antigen in human serum samples.
dc.description.sponsorshipScientific and Technological Research Council of by Tekirda.g Namik Kemal University, NKU-BAP [NKUBAP.00. ONAP.19.209]
dc.description.sponsorshipThis research was supported The Scientific and Technological Research Council of by Tekirda.g Namik Kemal University, NKU-BAP (Grant number: NKUBAP.00. ONAP.19.209).
dc.identifier.doi10.1016/j.snb.2020.128788
dc.identifier.issn0925-4005
dc.identifier.scopus2-s2.0-85090007896
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1016/j.snb.2020.128788
dc.identifier.urihttps://hdl.handle.net/20.500.12428/22679
dc.identifier.volume325
dc.identifier.wosWOS:000581627000027
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherElsevier Science Sa
dc.relation.ispartofSensors and Actuators B-Chemical
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectkallikrein 4
dc.subjectsuccinimide groups substituted polythiophene
dc.subjectpolymer
dc.subjectdisposable biosensor
dc.titleConstruction of succinimide group substituted polythiophene polymer functionalized sensing platform for ultrasensitive detection of KLK 4 cancer biomarker
dc.typeArticle

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