A novel ultrasensitive immunosensor based on disposable graphite paper electrodes for troponin T detection in cardiovascular disease

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dc.contributor.authorDemirbakan, Burcak
dc.contributor.authorSezgintürk, Mustafa Kemal
dc.date.accessioned2025-01-27T20:27:23Z
dc.date.available2025-01-27T20:27:23Z
dc.date.issued2020
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractTroponin T, which is a biomarker of cardiovascular disease, is vital for acute myocardial infarction (AMI). AMI is the most well-known cause of death in the world. In the present work, we designed a novel immunosensor using disposable, cheap, and very conductive graphite paper electrodes (GP). The proposed immunosensor was modified with hydrochloric acid (HCl) for immobilization of anti-TnT antibody. After carboxyl group formation on the surface, the GP electrodes were incubated with EDC/NHS pair as a cross-linker. Electrochemical impedance spectroscopy (EIS), single frequency impedance (SFI), cyclic voltammetry (CV) and square wave voltammetry (SWV) techniques were used for electrochemical characterization of the label-free immunosensor. Furthermore, the fabrication process of the proposed immunosensor was examined by using scanning electron microscopy (SEM). The proposed electrochemical immunosensor had a wide detection range (0.5 fg - 1000 fg/mL), and low limit of detection (LOD) and low limit of quantification (LOQ) at 1.28 fg/mL and 4.29 fg/mL, respectively. The designed immunosensor exhibited very good sensitivity, reproducibility, repeatability, reusability and long storage life. Additionally, the immunosensor was successfully applied to detection of TnT in human serum.
dc.identifier.doi10.1016/j.talanta.2020.120779
dc.identifier.issn0039-9140
dc.identifier.issn1873-3573
dc.identifier.pmid32200916
dc.identifier.scopus2-s2.0-85079331980
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1016/j.talanta.2020.120779
dc.identifier.urihttps://hdl.handle.net/20.500.12428/22673
dc.identifier.volume213
dc.identifier.wosWOS:000525711800001
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier
dc.relation.ispartofTalanta
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectGraphite paper (GP)
dc.subjectTroponin T (TnT)
dc.subjectBiosensor
dc.subjectSingle frequency impedance (SFI) technique
dc.titleA novel ultrasensitive immunosensor based on disposable graphite paper electrodes for troponin T detection in cardiovascular disease
dc.typeArticle

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