Isolation, Identification, and Pathogenicity of Vibrio gigantis Retrieved from European Seabass (Dicentrarchus labrax) Farmed in Türkiye

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dc.authoridSteinum, Terje Marken/0000-0001-5377-9765
dc.authoridYılmaz, Sevdan/0000-0002-4809-5809
dc.contributor.authorYılmaz, Sevdan
dc.contributor.authorKaratas, Sueheyla
dc.contributor.authorSteinum, Terje Marken
dc.contributor.authorGuerkan, Mert
dc.contributor.authorYilmaz, Dilek Kahraman
dc.contributor.authorAbdel-Latif, Hany M. R.
dc.date.accessioned2025-01-27T20:59:54Z
dc.date.available2025-01-27T20:59:54Z
dc.date.issued2023
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractIn this study, V. gigantis strain C24 was isolated from cases of winter mortalities of hatchery-reared European seabass (Dicentrarchus labrax) broodstock in T & uuml;rkiye. The first mortalities were reported in September 2016 and occurred annually in early autumn/late winter until the end of February 2019, when 15% of accumulated mortality was recorded. Diseased moribund fish exhibited general septicemic signs, including dermal ulcerations with hemorrhagic margins, distended abdomens, and hemorrhages below the pectorals, pelvic fins, and at the operculum. Postmortem findings showed congestion in several internal organs, hemorrhagic ascitic fluid, and congested prolapsed anal openings. The representative bacterial isolate V. gigantis strain C24 was characterized as Gram-negative, motile, nitrite-producing, and as vibrio static agent O/129-sensitive. The full-length 16S rRNA sequence (Accession No. ON778781) and gyrB gene sequence (Accession No. ON792326) of the C24 strain showed high similarity to V. gigantis strains. Moreover, the whole-genome average nucleotide identity (ANI) values (ANI > 97.7%) against four V. gigantis strains above the species demarcation limit unambiguously identified the C24 isolate as a member of this species. A preliminary virulence-gene analysis showed that the V. gigantis isolate C24 encoded at least three exotoxins, including two aerolysins and a thermolabile hemolysin. The experimental infection showed that the C24 isolate exhibited low to moderate virulence in experimentally infected European seabass juveniles. Interestingly, antimicrobial susceptibility testing revealed that the C24 isolate was susceptible to nalidixic acid, ciprofloxacin, and several other antibiotics but resistant to tilmicosin, kanamycin, streptomycin, and ampicillin. To our knowledge, this study is the first to report that V. gigantis could be considered an emerging bacterial pathogen in T & uuml;rkiye, and it may threaten the international European seabass production.
dc.identifier.doi10.3390/ani13223580
dc.identifier.issn2076-2615
dc.identifier.issue22
dc.identifier.pmid38003197
dc.identifier.scopus2-s2.0-85178326143
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.3390/ani13223580
dc.identifier.urihttps://hdl.handle.net/20.500.12428/26876
dc.identifier.volume13
dc.identifier.wosWOS:001120145700001
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherMdpi
dc.relation.ispartofAnimals
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WoS_20250125
dc.subjectDicentrarchus labrax
dc.subjecthistopathology
dc.subjectmortalities
dc.subjectvibriosis
dc.subjectVibrio gigantis
dc.titleIsolation, Identification, and Pathogenicity of Vibrio gigantis Retrieved from European Seabass (Dicentrarchus labrax) Farmed in Türkiye
dc.typeArticle

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