Characterization of an unusual cytoplasmic chimera detected in bolting garlic clones

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dc.authoridSimon, Philipp/0000-0001-6978-6062
dc.authoridSenalik, Douglas/0000-0001-8526-0554
dc.authoridIPEK, MERYEM/0000-0002-0609-3442
dc.contributor.authorIpek, Meryem
dc.contributor.authorIpek, Ahmet
dc.contributor.authorSenalik, Douglas
dc.contributor.authorSimon, Philipp W.
dc.date.accessioned2025-01-27T20:45:37Z
dc.date.available2025-01-27T20:45:37Z
dc.date.issued2007
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractProduction of a visible flower stalk, or bolting, has been used as a major trait to categorize garlic (Allium sativum L.) clones. Analysis of mitochondrial genome variation with polymerase chain reaction (PCR) revealed differences between bolting and nonbolting clones of garlic. Screening 333 garlic accessions from diverse geographic origins revealed a 1403-bp mitochondrial DNA marker associated with bolting that the authors call Bolt Marker (BltM). Bolt Marker did not amplify in any of the 131 nonbolting clones, whereas amplification of this marker was observed in 127 of 130 (97.7%) garlic clones that bolted completely in Wisconsin. Seventy-two garlic clones bolted incompletely (clones in which some but not all of the plants bolted), and this marker was not amplified in 69 (95.8%) of these clones. Because of the significant association of BltM with bolting, this PCR-based marker can be used to discriminate complete-bolting garlic clones reliably from nonbolting and incomplete-bolting ones. Sequence characterization of this marker revealed that BltM is a chimera involving both mitochondrial and chloroplast DNA. The DNA sequences including and flanking both the 5' and 3' ends of this marker are consistent with an approximate to 4.8-kbp chloroplast DNA fragment having been inserted into the mitochondrial genome downstream from the mitochondrial cox3 gene. Sequence alignment of the chloroplast genes in this chimeric region with the homologous sequences in GenBank indicate the presence of deletions, insertions, and single nucleotide polymorphisms in the coding sequences, resulting in putative, incomplete open reading frames or frame shift mutations. Hence, the authors speculate that this insertion may have occurred long ago in the evolution of garlic.
dc.identifier.doi10.21273/JASHS.132.5.664
dc.identifier.endpage669
dc.identifier.issn0003-1062
dc.identifier.issn2327-9788
dc.identifier.issue5
dc.identifier.scopus2-s2.0-36649016238
dc.identifier.scopusqualityQ1
dc.identifier.startpage664
dc.identifier.urihttps://doi.org/10.21273/JASHS.132.5.664
dc.identifier.urihttps://hdl.handle.net/20.500.12428/24656
dc.identifier.volume132
dc.identifier.wosWOS:000249178800012
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherAmer Soc Horticultural Science
dc.relation.ispartofJournal of The American Society For Horticultural Science
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WoS_20250125
dc.subjectAllium sativum
dc.subjectmarker
dc.subjectflowering
dc.subjectmitochondria
dc.subjectplastid
dc.titleCharacterization of an unusual cytoplasmic chimera detected in bolting garlic clones
dc.typeArticle

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