Single-stranded DNA (ssDNA) Aptamer targeting SipA protein inhibits Salmonella Enteritidis invasion of intestinal epithelial cells

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dc.authoridEvran, Serap/0000-0001-6676-4888
dc.authoridShatila, Fatima/0000-0001-9246-8847
dc.authoridCAKIR, BANU/0000-0001-6645-6527
dc.contributor.authorShatila, Fatima
dc.contributor.authorYalcin, H. Tansel
dc.contributor.authorOzyurt, Canan
dc.contributor.authorEvran, Serap
dc.contributor.authorCakir, Busra
dc.contributor.authorYasa, Ihsan
dc.contributor.authorNalbantsoy, Ayse
dc.date.accessioned2025-01-27T20:39:26Z
dc.date.available2025-01-27T20:39:26Z
dc.date.issued2020
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractSalmonella Enteritidis is an important pathogen that can invade the intestinal cells of its host causing salmonellosis. SipA protein, an effector protein secreted by T3SS, maintains invasion of host cells more efficient. Thus, inhibitory aptamers against SipA protein were developed using magnetic bead-based Systematic Evolution of Ligands by Exponential Enrichment (SELEX) method. The enriched sequences were obtained after 9 SELEX rounds. Among which, an aptamer namely Apt17 displayed Kd values equivalent to 114.9 and 63.4 nM at 27 degrees C and 37 degrees C, respectively. The effect of Apt17 on adhesion and invasion of Caco-2 cells by the tested strains was determined. While the adhesion and invasion of Salmonella Enteritidis TM 6 were inhibited by 70% and 37.7%, those of Salmonella Enteritidis TM 68 were inhibited by 45.71% and 39.5% respectively. These results represent a corner stone for future studies that could aim to develop putative inhibitors against Salmonellosis. (C) 2020 Elsevier B.V. All rights reserved.
dc.description.sponsorshipScientific Research Unit of Ege University [2017-FEN-038]
dc.description.sponsorshipThis work was financially supported by the Scientific Research Unit of Ege University (Project number: 2017-FEN-038).
dc.identifier.doi10.1016/j.ijbiomac.2020.01.132
dc.identifier.endpage524
dc.identifier.issn0141-8130
dc.identifier.issn1879-0003
dc.identifier.pmid31953175
dc.identifier.scopus2-s2.0-85078132832
dc.identifier.scopusqualityQ1
dc.identifier.startpage518
dc.identifier.urihttps://doi.org/10.1016/j.ijbiomac.2020.01.132
dc.identifier.urihttps://hdl.handle.net/20.500.12428/23948
dc.identifier.volume148
dc.identifier.wosWOS:000522094600052
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier
dc.relation.ispartofInternational Journal of Biological Macromolecules
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectAptamer
dc.subjectSalmonella invasion
dc.subjectSalmonella invasion protein A
dc.titleSingle-stranded DNA (ssDNA) Aptamer targeting SipA protein inhibits Salmonella Enteritidis invasion of intestinal epithelial cells
dc.typeArticle

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