Minimization of Interferences in Flow Injection Amperometric Glucose Biosensor Based on Oxidation of Enzymatically-produced H2O2

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dc.authoridDilgin, Yusuf/0000-0002-2980-6871
dc.authoridKarakaya, Serkan/0000-0002-6401-3295
dc.contributor.authorKarakaya, Serkan
dc.contributor.authorDilgin, Yusuf
dc.date.accessioned2025-01-27T20:59:40Z
dc.date.available2025-01-27T20:59:40Z
dc.date.issued2019
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractOne of the major problems in amperometric biosensors based on detection of H2O2 produced by enzymatic reaction between oxidase enzymes and substrate is the interference of redox active compounds such as ascorbic acid (AA), dopamine (DA) and uric acid (UA). To minimize these interferences, sodium bismuthate was used for the first time as an insoluble pre-oxidant in the flow injection (FI) amperometric glucose biosensor at a Glucose oxidase (GOx) immobilized Pt/Pd bimetallic modified pre-anodized pencil graphite electrode (p.PGE). In this context, these interfering compounds were injected into a flow injection analysis (FIA) system using an injector which was filled with NaBiO3. Thus, these interferents were converted into their redox inactive oxidized forms before reaching the electrode in the flow cell. While glucose was not influenced by the pre-oxidant in the injector, the huge oxidation peak currents of the interferents decreased significantly in the biosensor. FI amperometric current time curves showed that the AA, DA and UA were minimized by 96 %, 86 %, and 98 % respectively, in the presence of an equivalent concentration of interferences in a 1.0 mM glucose solution. The proposed FI amperometric glucose biosensor exhibits a wide linear range (0.01-10 mM, R-2=0.9994) with a detection limit of 2.4x10(-3) mM. Glucose levels in the artificial serum and two real samples were successfully determined using the fabricated FI amperometric biosensor.
dc.description.sponsorshipAcademic Training Program (OYP); Scientific and Technological Research Council of Turkey (TUBITAK)
dc.description.sponsorshipSerkan Karakaya thanks the Academic Training Program (OYP) for financial support and the Scientific and Technological Research Council of Turkey (TUBITAK) for their grant. This study was produced from part of the PhD thesis of Serkan Karakaya. Mr G. H. Lee is thanked for proof-reading the final manuscript.
dc.identifier.doi10.1002/elan.201800887
dc.identifier.endpage1380
dc.identifier.issn1040-0397
dc.identifier.issn1521-4109
dc.identifier.issue7
dc.identifier.scopus2-s2.0-85064504530
dc.identifier.scopusqualityQ2
dc.identifier.startpage1373
dc.identifier.urihttps://doi.org/10.1002/elan.201800887
dc.identifier.urihttps://hdl.handle.net/20.500.12428/26774
dc.identifier.volume31
dc.identifier.wosWOS:000476550300022
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherWiley-V C H Verlag Gmbh
dc.relation.ispartofElectroanalysis
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectGlucose biosensor
dc.subjectPencil graphite electrode
dc.subjectGlucose oxidase
dc.subjectInterference minimization
dc.subjectFlow injection analysis
dc.subjectPre-oxidant
dc.titleMinimization of Interferences in Flow Injection Amperometric Glucose Biosensor Based on Oxidation of Enzymatically-produced H2O2
dc.typeArticle

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