A Highly Selective Poly(thiophene)-graft-Poly(methacrylamide) Polymer Modified ITO Electrode for Neuron Specific Enolase Detection in Human Serum

Basit Öğe Kaydı
dc.authoridAYDIN, MUHAMMET/0000-0002-8716-9970
dc.contributor.authorAydin, Muhammet
dc.contributor.authorAydin, Elif Burcu
dc.contributor.authorSezgintürk, Mustafa Kemal
dc.date.accessioned2025-01-27T20:31:32Z
dc.date.available2025-01-27T20:31:32Z
dc.date.issued2019
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractIn this study, an impedimetric immunosensor based on polymer poly(thiophene)-graft-poly(methacrylamide) polymer (P(Thi-g-MAm)) modified indium tin oxide (ITO) electrode is developed for the detection of the Neuron Specific Enolase (NSE) cancer biomarker. First, the P(Thi-g-MAm) polymer is synthesized and coated on the ITO electrode by using a spin-coating technique. P(Thi-g-MAm) polymer acts as an immobilization platform for immobilization of NSE-specific monoclonal antibodies. Anti-NSE antibodies are utilized as biosensing molecules and they bind to the amino groups of P(Thi-g-Mam) polymer via glutaraldehyde cross-linking. Spin-coating technique is employed for bioelectrode fabrication and this technique provides a thin and uniform film on the ITO electrode surface. This bioelectrode fabrication technique is simple and it generates a suitable platform for large-scale loadings of anti-NSE antibodies. This immunosensor exhibits a wide linear detection range from 0.02 to 4 pg mL(-1) and with an ultralow detection limit of 6.1 fg mL(-1). It reveals a good long-term stability (after 8 weeks, 78% of its initial activity), an excellent reproducibility (1.29% of relative standard deviation (RSD)), a good repeatability (5.55% of RSD), and a high selectivity. In addition, the developed immunosensor is proposed as a robust diagnostic tool for the clinical detection of NSE and other cancer biomarkers.
dc.identifier.doi10.1002/mabi.201900109
dc.identifier.issn1616-5187
dc.identifier.issn1616-5195
dc.identifier.issue8
dc.identifier.pmid31222894
dc.identifier.scopus2-s2.0-85067867205
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1002/mabi.201900109
dc.identifier.urihttps://hdl.handle.net/20.500.12428/23188
dc.identifier.volume19
dc.identifier.wosWOS:000479103500001
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherWiley-V C H Verlag Gmbh
dc.relation.ispartofMacromolecular Bioscience
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectdisposable electrodes
dc.subjectelectrochemical impedance spectroscopy
dc.subjectgraft polymer
dc.subjectlung cancer
dc.subjectneuron-specific enolase
dc.titleA Highly Selective Poly(thiophene)-graft-Poly(methacrylamide) Polymer Modified ITO Electrode for Neuron Specific Enolase Detection in Human Serum
dc.typeArticle

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu