Saccharomyces boulardii ameliorates clarithromycin- and methotrexate-induced intestinal and hepatic injury in rats

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dc.authoridCan, Guray/0000-0002-6054-9244
dc.authoridOzdemir Kumral, Zarife Nigar/0000-0002-9485-0174
dc.authoridYegen, Berrak/0000-0003-0791-0165
dc.contributor.authorDuman, Deniz Guney
dc.contributor.authorKumral, Zarife Nigar Ozdemir
dc.contributor.authorErcan, Feriha
dc.contributor.authorDeniz, Mustafa
dc.contributor.authorCan, Guray
dc.contributor.authorYegen, Berrak Caglayan
dc.date.accessioned2025-01-27T20:14:13Z
dc.date.available2025-01-27T20:14:13Z
dc.date.issued2013
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractSaccharomyces boulardii is a probiotic used for the prevention of antibiotic-associated diarrhoea. We aimed to investigate whether S. boulardii could alter the effects of clarithromycin (CLA) and methotrexate (MTX) on oro-caecal intestinal transit and oxidative damage in rats. Rats were divided into two groups receiving a single dose of MTX (20 mg/kg) or CLA (20 mg/kg per d) for 1 week. Groups were treated with either saline or S. boulardii (500 mg/kg) twice per d throughout the experiment. The control group was administered only saline. Following decapitation, intestinal transit and inflammation markers of glutathione (GSH), malondialdehyde and myeloperoxidase were measured in intestinal and hepatic tissues. CLA and MTX increased intestinal transit, while S. boulardii treatment slowed down CLA-facilitated transit back to control level. Both MTX and CLA increased lipid peroxidation while depleting the antioxidant GSH content in the hepatic and ileal tissues. Conversely, lipid peroxidation was depressed and GSH levels were increased in the ileal and hepatic tissues of S. boulardii-treated rats. Increased ileal neutrophil infiltration due to MTX and CLA treatments was also reduced by S. boulardii treatment. Histological analysis supported that S. boulardii protected intestinal tissues against the inflammatory effects of both agents. These findings suggest that S. boulardii ameliorates intestinal injury and the accompanying hepatic inflammation by supporting the antioxidant state of the tissues and by inhibiting the recruitment of neutrophils. Moreover, a preventive effect on MTX-induced toxicity is a novel finding of S. boulardii, proposing it as an adjunct to chemotherapy regimens.
dc.identifier.doi10.1017/S000711451200517X
dc.identifier.endpage499
dc.identifier.issn0007-1145
dc.identifier.issn1475-2662
dc.identifier.issue3
dc.identifier.pmid23279717
dc.identifier.scopus2-s2.0-84880830807
dc.identifier.scopusqualityQ1
dc.identifier.startpage493
dc.identifier.urihttps://doi.org/10.1017/S000711451200517X
dc.identifier.urihttps://hdl.handle.net/20.500.12428/20996
dc.identifier.volume110
dc.identifier.wosWOS:000322276800012
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherCambridge Univ Press
dc.relation.ispartofBritish Journal of Nutrition
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WoS_20250125
dc.subjectSaccharomyces boulardii
dc.subjectClarithromycin
dc.subjectMethotrexate
dc.subjectIntestinal toxicity
dc.subjectHepatic toxicity
dc.titleSaccharomyces boulardii ameliorates clarithromycin- and methotrexate-induced intestinal and hepatic injury in rats
dc.typeArticle

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