Comparison of dichloran rose bengal chloramphenicol and Sabouraud dextrose agar with cycloheximide and chloramphenicol for airborne mold sampling

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dc.authoridMentese, Sibel/0000-0002-0395-3603
dc.contributor.authorMentese, Sibel
dc.contributor.authorOtkun, Muserref Tatman
dc.contributor.authorPalaz, Elif
dc.date.accessioned2025-01-27T20:38:54Z
dc.date.available2025-01-27T20:38:54Z
dc.date.issued2017
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractThe more the mold species isolated on a culture medium, the more the sampling environment is represented accurately. According to the sampling purpose, it is crucial to use the best culture medium for mold. However, no study is available regarding the comparison of dichloran rose bengal chloramphenicol (DRBC) and Sabouraud dextrose agar with cycloheximide and chloramphenicol (SDA-CHX-CHL) culture media in terms of their application for airborne sampling, isolation, and identification of fungi. Airborne mold samples were impacted onto both DRBC and SDA-CHX-CHL, simultaneously using single-stage Andersen sampler. The limit of detection (LOD) value for airborne mold count was 7 CFU m(-3) (1 colony growth on the Petri dish). The total mold counts (TMC) ranged between < 7 and 504 CFU m(-3) (med 56 CFU m(-3)) and < 7 and 1218 CFU m(-3) (med 259 CFU m(-3)), collected on SDA-CHX-CHL and DRBC, respectively. Significantly higher TMC were observed on DRBC than on SDA regardless of the sampling environment (i.e, indoor or outdoor) (p < 0.05). Among the most predominant mold genera, observation frequencies of Penicillium spp. and Aspergillus spp. on both culture media were found to be more than 70%. Observation frequencies of Cladosporium spp., Alternaria spp., and yeast were found to be higher in samples collected on DRBC than those on SDA-CHX-CHL. Finally, DRBC was found to be superior to SDA in terms of both number of colonies and number of genera isolated from the air.
dc.description.sponsorshipScientific and Technological Council of Turkey (TUBITAK) [112Y059]
dc.description.sponsorshipThis study was supported financially by The Scientific and Technological Council of Turkey (TUBITAK). Project No: 112Y059. Authors also thank to Osman Cotuker and Deniz Tasdibi for field studies and to Catherine Yigit for English proofreading.
dc.identifier.doi10.1007/s10453-016-9462-2
dc.identifier.endpage219
dc.identifier.issn0393-5965
dc.identifier.issn1573-3025
dc.identifier.issue2
dc.identifier.scopus2-s2.0-84994338589
dc.identifier.scopusqualityQ2
dc.identifier.startpage211
dc.identifier.urihttps://doi.org/10.1007/s10453-016-9462-2
dc.identifier.urihttps://hdl.handle.net/20.500.12428/23795
dc.identifier.volume33
dc.identifier.wosWOS:000401449000004
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofAerobiologia
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectAirborne mold sampling
dc.subjectChloramphenicol (CHL)
dc.subjectCulture medium
dc.subjectDichloran rose bengal chloramphenicol (DRBC) agar
dc.subjectIndoor air
dc.subjectOutdoor air
dc.subjectSabouraud dextrose agar (SDA)
dc.titleComparison of dichloran rose bengal chloramphenicol and Sabouraud dextrose agar with cycloheximide and chloramphenicol for airborne mold sampling
dc.typeArticle

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