Micronuclei, Nucleoplasmic Bridges, and Nuclear Buds Induced in Human Lymphocytes by the Fungicide Signum and Its Active Ingredients (Boscalid and Pyraclostrobin)

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dc.authoridCAYIR, AKIN/0000-0002-2014-6635
dc.contributor.authorCayir, Akin
dc.contributor.authorCoskun, Munevver
dc.contributor.authorCoskun, Mahmut
dc.date.accessioned2025-01-27T20:14:35Z
dc.date.available2025-01-27T20:14:35Z
dc.date.issued2014
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractThe aim of this study was to investigate the genotoxic and cytotoxic potential of the Signum fungicide and its active ingredients (boscalid and pyraclostrobin) on human peripheral blood lymphocytes using the cytokinesis-block micronucleus (CBMN) assay. Micronuclei (MNi), nucleoplasmic bridges (NPBs), nuclear bud (NBUDs) formations, and the cytokinesis-block proliferation index (CBPI) were evaluated in treated lymphocytes in Go (cells were treated and then kept in culture without stimulation for 24 h) and proliferation phases (cells were treated after 44 h culture in medium containing phytohemagglutinin). MN formation in lymphocytes treated in G(0) statistically increased at doses of 2, 6, and 25 mu g/mL signum; 0.5 and 2 mu g/mL boscalid; and 0.5, 1.5, and 2 mu g/mL pyraclostrobin; while NPB formation increased at a dose of 0.25 mu g/mL pyraclostrobin. All concentrations of each fungicide did not statistically increase NBUD formation, while the cytotoxicity increased the dependent on concentration in lymphocytes treated in G0. Doses of 0.5, 1, 1.5, and 3 mu g/mL signum; 0.5, 1, and 1.5 mu g/mL boscalid; and 0.75 mu g/mL pyraclostrobin statistically increased the MN formation in proliferating lymphocytes. NPB formation increased in proliferating lymphocytes at doses of 1, 1.5, 2, and 3 mu g/mL signum and at a dose of 0.75 mu g/mL pyraclostrobin. In addition, a dose of 0.75 mu g/mL pyraclostrobin increased NBUD frequencies. Cytotoxicity increased with increasing concentrations of each fungicide. It is concluded that signum, boscalid, and pyraclostrobin may be genotoxic and cytotoxic in vitro human peripheral blood lymphocytes in consideration of each of the two protocols. (C) 2012 Wiley Periodicals, Inc.
dc.description.sponsorshipCanakkale Onsekiz Mart University (Scientific Research Project Commission) [2009/45]
dc.description.sponsorshipContract grant sponsor: Canakkale Onsekiz Mart University (Scientific Research Project Commission) Contract grant number: 2009/45
dc.identifier.doi10.1002/tox.21789
dc.identifier.endpage732
dc.identifier.issn1520-4081
dc.identifier.issn1522-7278
dc.identifier.issue7
dc.identifier.pmid22730168
dc.identifier.scopus2-s2.0-84901842679
dc.identifier.scopusqualityQ1
dc.identifier.startpage723
dc.identifier.urihttps://doi.org/10.1002/tox.21789
dc.identifier.urihttps://hdl.handle.net/20.500.12428/21143
dc.identifier.volume29
dc.identifier.wosWOS:000337515100001
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherWiley-Blackwell
dc.relation.ispartofEnvironmental Toxicology
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectmicronucleus
dc.subjectnucleoplasmic bridge
dc.subjectnuclear bud
dc.subjectsignum
dc.subjectboscalid
dc.titleMicronuclei, Nucleoplasmic Bridges, and Nuclear Buds Induced in Human Lymphocytes by the Fungicide Signum and Its Active Ingredients (Boscalid and Pyraclostrobin)
dc.typeArticle

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