DNA damage and circulating cell free DNA in greenhouse workers exposed to pesticides

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dc.authoridCAYIR, AKIN/0000-0002-2014-6635
dc.contributor.authorCayir, Akin
dc.contributor.authorCoskun, Mahmut
dc.contributor.authorCoskun, Munevver
dc.contributor.authorCobanoglu, Hayal
dc.date.accessioned2025-01-27T20:16:44Z
dc.date.available2025-01-27T20:16:44Z
dc.date.issued2018
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractPesticides widely used in agriculture and other applications have been linked to cancer and other diseases through several potential mechanisms. The goals of this study were to assess DNA damage in lymphocytes by the cytokinesis-block micronucleus assay (CBMN), and to measure circulating cell free DNA (ccf-DNA) in the blood of pesticide-exposed greenhouse workers and matched controls living in the same area. CBMN was applied to peripheral blood lymphocyte samples taken at different times (spring and autumn) for each individual. We measured plasma ccf-DNA levels using a Qubit((R)) fluorometer. The results indicated that the MNL, BNMN, and NBUDs frequencies of pesticide-exposed individuals were significantly higher than non-exposed individuals. Apart from MNL, BNMN and CBPI, a season-related effect was found for the NPB and NBUD frequencies. With MNL and BNMN as the dependent variables, multiple regression analysis showed that age and gender affected MN formation. The ccf-DNA level in the pesticide-exposed group was significantly higher than the control group. There was no seasonal variation regarding the free DNA amount. Ccf-DNA in males was found to be higher than females. The MNL, BNMN, NPB, and CBPI did not correlate with the ccf-DNA amount. It can be concluded that pesticide exposure can modulate DNA integrity via different mechanisms. Also, elevated levels of ccf-DNA could be recommended as a biomarker of pesticide exposure. Environ. Mol. Mutagen. 59:161-169, 2018. (c) 2017 Wiley Periodicals, Inc.
dc.description.sponsorshipCanakkale Onsekiz Mart Universitesi [2010/128]
dc.description.sponsorshipGrant sponsor: Canakkale Onsekiz Mart Universitesi; Grant number: 2010/128.
dc.identifier.doi10.1002/em.22148
dc.identifier.endpage169
dc.identifier.issn0893-6692
dc.identifier.issn1098-2280
dc.identifier.issue2
dc.identifier.pmid29130594
dc.identifier.scopus2-s2.0-85033604508
dc.identifier.scopusqualityQ2
dc.identifier.startpage161
dc.identifier.urihttps://doi.org/10.1002/em.22148
dc.identifier.urihttps://hdl.handle.net/20.500.12428/21368
dc.identifier.volume59
dc.identifier.wosWOS:000424892000008
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherWiley
dc.relation.ispartofEnvironmental and Molecular Mutagenesis
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectcirculating cell free DNA
dc.subjectmicronucleus
dc.subjectnucleoplasmic bridge
dc.subjectpesticides
dc.subjectgreenhouse workers
dc.titleDNA damage and circulating cell free DNA in greenhouse workers exposed to pesticides
dc.typeArticle

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