Antiproliferative activity of Humulus lupulus extracts on human hepatoma (Hep3B), colon (HT-29) cancer cells and proteases, tyrosinase, ?-lactamase enzyme inhibition studies

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dc.authoridComert Onder, Ferah/0000-0002-4037-1979
dc.contributor.authorOnder, Ferah Comert
dc.contributor.authorAy, Mehmet
dc.contributor.authorTurkoglu, Sumeyye Aydogan
dc.contributor.authorKockar, Feray Tura
dc.contributor.authorCelik, Ayhan
dc.date.accessioned2025-01-27T20:52:29Z
dc.date.available2025-01-27T20:52:29Z
dc.date.issued2016
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractThe aims of this study were to examine the antiproliferation of Humulus lupulus extracts on human hepatoma carcinoma (Hep3B) and human colon carcinoma (HT-29) cell lines along with enzyme inhibitory effects of the crude extracts. Potential cell cytotoxicity of six different H. lupulus extracts were assayed on various cancer cells using MTT assay at 24, 48 and 72h intervals. Methanol-1 extract has inhibited the cell proliferation with doses of 0.6-1mg/mL in a time dependent (48 and 72 hours) manner in Hep3B cells with 70% inhibition, while inhibitory effect was not seen in colon cancer cells. Acetone extract has increased the cell proliferation at low doses of 0.1mg/mL for 72h in Hep3B cells and 0.1-0.2mg/mL for 48 and 72h in HT29 cells. The inhibitory effects of the extracts were compared by relative maximum activity values (V-max) using proteases such as -chymotrypsin, trypsin and papain, tyrosinase and -lactamase (penicillinase).
dc.description.sponsorshipCanakkale Onsekiz Mart University [BAP 2010/224]
dc.description.sponsorshipWe acknowledge the Canakkale Onsekiz Mart University for financial support (Research University Grant BAP 2010/224).
dc.identifier.doi10.3109/14756366.2015.1004060
dc.identifier.endpage98
dc.identifier.issn1475-6366
dc.identifier.issn1475-6374
dc.identifier.issue1
dc.identifier.scopus2-s2.0-84953209715
dc.identifier.scopusqualityQ1
dc.identifier.startpage90
dc.identifier.urihttps://doi.org/10.3109/14756366.2015.1004060
dc.identifier.urihttps://hdl.handle.net/20.500.12428/25775
dc.identifier.volume31
dc.identifier.wosWOS:000367555300010
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherTaylor & Francis Ltd
dc.relation.ispartofJournal of Enzyme Inhibition and Medicinal Chemistry
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectAntiproliferation
dc.subjectenzyme inhibition
dc.subjectHep3B
dc.subjecthop female cones
dc.subjectHT-29
dc.subjectHumulus lupulus
dc.subjectproteases
dc.subjecttyrosinase
dc.subjectbeta-lactamase (penicillinase)
dc.titleAntiproliferative activity of Humulus lupulus extracts on human hepatoma (Hep3B), colon (HT-29) cancer cells and proteases, tyrosinase, ?-lactamase enzyme inhibition studies
dc.typeArticle

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