First characterization of the complete mitochondrial genome of fungal plant-pathogen Monilinia laxa which represents the mobile intron rich structure

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dc.contributor.authorYildiz, Gozde
dc.contributor.authorOzkilinc, Hilal
dc.date.accessioned2025-01-27T20:34:43Z
dc.date.available2025-01-27T20:34:43Z
dc.date.issued2020
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractMonilinia laxa is an important fungal plant pathogen causing brown rot on many stone and pome fruits worldwide. Mitochondrial genome (mitogenome) plays a critical role in evolutionary biology of the organisms. This study aimed to characterize the complete mitogenome of M. laxa by using next-generation sequencing and approaches of de novo assembly and annotation. The total length of the mitogenome of M. laxa was 178,357 bp, and its structure was circular. GC content of the mitogenome was 30.1%. Annotation of the mitogenome presented 2 ribosomal RNA (rRNA) genes, 32 transfer RNA genes (tRNA), 1 gene encoding mitochondrial ribosomal protein S3, 14 protein-coding genes and 15 open reading frame encoding hypothetical proteins. Moreover, the group I mobile introns encoding homing endonucleases including LAGLIDADG and GIY-YIG families were found both within coding regions (genic) and intergenic regions of the mitogenome, indicating an enlarged size and a dynamic structure of the mitogenome. Furthermore, a comparative mitogenomic analysis was performed between M. laxa and the three closely related fungal phytopathogen species (Botryotinia fuckeliana, Sclerotinia sclerotiorum and, S. borealis). Due to the number and distribution of introns, the large extent of structural rearrangements and diverse mitogenome sizes were detected among the species investigated. Monilinia laxa presented the highest number of homing endonucleases among the fungal species considered in the analyses. This study is the first to report a detailed annotation of the mitogenome of an isolate of M. laxa, providing a solid basis for further investigations of mitogenome variations for the other Monilinia pathogens causing brown rot disease.
dc.description.sponsorshipTUBITAK (Scientific and Technological Research Council of Turkey) [217Z134]
dc.description.sponsorshipThis study was supported by TUBITAK (Scientific and Technological Research Council of Turkey) Project No. 217Z134 granted to Dr. H. OZKILINC.
dc.identifier.doi10.1038/s41598-020-70611-z
dc.identifier.issn2045-2322
dc.identifier.issue1
dc.identifier.pmid32788650
dc.identifier.scopus2-s2.0-85089372864
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1038/s41598-020-70611-z
dc.identifier.urihttps://hdl.handle.net/20.500.12428/23436
dc.identifier.volume10
dc.identifier.wosWOS:000563536300004
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherNature Portfolio
dc.relation.ispartofScientific Reports
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WoS_20250125
dc.subjectGroup-I Intron
dc.subjectGene
dc.subjectSequence
dc.subjectFructicola
dc.subjectOphiostoma
dc.subjectEvolution
dc.subjectInvasion
dc.subjectElements
dc.subjectProgram
dc.titleFirst characterization of the complete mitochondrial genome of fungal plant-pathogen Monilinia laxa which represents the mobile intron rich structure
dc.typeArticle

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