A new donor-acceptor conjugated polymer-gold nanoparticles biocomposite materials for enzymatic determination of glucose

Basit Öğe Kaydı
dc.authoridTAN, Burcu/0000-0003-4661-9661
dc.contributor.authorTan, Burcu
dc.contributor.authorBaycan, Fatma
dc.date.accessioned2025-01-27T20:22:35Z
dc.date.available2025-01-27T20:22:35Z
dc.date.issued2020
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractIn this study, the monomer 3-(5,8-bis (2,3-dihydrothieno[3,4-b][1,4]dioxin-5-yl) -3-(9hexyl-9H-carbazole-3-yl) quinoxalin-2-yl)-9-hexyl9H-carbazole (HCQE) was electropolymerized on the graphite pencil electrode for the first time and used as a glucose biosensor. After electropolymerization, enrichment with Au nanoparticles was performed to amplify the signal (PHCQE/AuNPs). Following the electropolymerization step, glucose oxidase (GOx) immobilization was performed on the conjugated polymer coated graphite pencil electrode (GPE) surface using glutaraldehyde (GA) as crosslinking reagent due to strong pi-pi* conjugation on the polymer backbone (PHCQE/AuNPs/GOx). Voltammetric determination was also performed by monitoring the oxygen consumption against Ag/AgCl reference electrode at-0.7 V in phosphate buffer solution (0.10 mM; pH 7.4). The new PHCQE/ AuNPs/GOx biosensor showed a linear response for glucose in the concentration range between 0.75 and 3.125 mM (LOD: 0.017 mM) along with a good sensitivity (0.13 mu A/mM(-1)). To examine the selectivity of the PHCQE/ AuNPs/GOx biosensor, citric acid, fructose, sucrose, galactose, L-ascorbic acid, mannose, dopamine and salicylic acid were studied in the same buffer solution. The conjugated polymer PHCQE enriched with Au nano particles provides enzyme immobilization effectively. The applicability of the biosensor in the commercial samples has been also investigated and we have found that the conjugated polymer PHCQE enriched with Au nano particles is very effective for the determination of glucose in commercial samples.
dc.identifier.doi10.1016/j.polymer.2020.123066
dc.identifier.issn0032-3861
dc.identifier.issn1873-2291
dc.identifier.scopus2-s2.0-85091646992
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1016/j.polymer.2020.123066
dc.identifier.urihttps://hdl.handle.net/20.500.12428/21961
dc.identifier.volume210
dc.identifier.wosWOS:000592402800006
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherElsevier Sci Ltd
dc.relation.ispartofPolymer
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectConjugated polymers
dc.subjectGlucose biosensor
dc.subjectElectropolymerization
dc.titleA new donor-acceptor conjugated polymer-gold nanoparticles biocomposite materials for enzymatic determination of glucose
dc.typeArticle

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu