Molecular phylogenetic species in Alternaria pathogens infecting pistachio and wild relatives

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dc.authoridOzkilinc, Hilal/0000-0003-0791-975X
dc.contributor.authorOzkilinc, Hilal
dc.contributor.authorSevinc, Unal
dc.date.accessioned2025-01-27T20:14:58Z
dc.date.available2025-01-27T20:14:58Z
dc.date.issued2018
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractMany important pathogens of crops worldwide are members of section Alternaria within the genus Alternaria. Representative species in this section such as Alternaria alternata, Alternaria tenuissima, and Alternaria arborescens show high variability, intermediate characters and plasticity in morphological features, which makes species identification difficult. The aim of this study was to characterize Alternaria species associated with pistachio and wild relatives in Turkey using molecular phylogenetics. One hundred isolates of Alternaria spp. from pistachio and wild relatives from Turkey were investigated. In addition, standard morphological reference isolates and Alternaria blight pathogens of pistachio from USA were included. Sequence data from major allergen a1, ATPase, endopolygalacturanase, and anonymous regions OPA1.3 and SCAR2 were obtained. Gene trees were estimated based on maximum parsimony, maximum-likelihood, and bayesian inference methods. Species tree estimation was performed based on Yule speciation and strict molecular clock assumption. Among the collection of Alternaria spp. from Turkey, only one A. arborescens isolate and three isolates which were morphologically A. alternata/A. arborescens intermediate types, but, phylogenetically close to A. arborescens were discovered. While A. alternata and A. tenuissima formed one phylogenetic species, A. alternata/tenuissima were phylogenetically distinct from A. arborescens. Furthermore, a TaqI restriction site in the endopolygalacturanase gene was explored as a novel diagnosis for identification of A. alternata/tenuissima and A. arborescens. All these molecular phylogenetic approaches allow to distinguish morphologically similar Alternaria pathogens and molecular phylogenies of Alternaria pathogens from pistachio and wild relatives in Turkey are described for the first time.
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK) [TUBITAK 112O554]
dc.description.sponsorshipThis research was supported by The Scientific and Technological Research Council of Turkey (TUBITAK), Grant of TUBITAK 112O554 project. The authors would like to thank the researchers of the project for their work in sampling, isolations, pathogenicity which was presented in another publication. The authors would like to thank Professor Tobin Peever (Dept. of Plant Pathology, Washington State University, Pullman, USA) for his review and valuable comments, and Professor Barry M. Pryor (University of Arizona, Dept. of Plant Pathology, Tucson, Arizona/USA) for providing reference isolates as well as Alternaria spp. isolates from pistachio.
dc.identifier.doi10.1007/s13205-018-1277-0
dc.identifier.issn2190-572X
dc.identifier.issn2190-5738
dc.identifier.issue5
dc.identifier.pmid29755919
dc.identifier.scopus2-s2.0-85046748416
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1007/s13205-018-1277-0
dc.identifier.urihttps://hdl.handle.net/20.500.12428/21249
dc.identifier.volume8
dc.identifier.wosWOS:000432064300001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSpringer Heidelberg
dc.relation.ispartof3 Biotech
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WoS_20250125
dc.subjectAlternaria
dc.subjectAlternaria blight
dc.subjectPhylogenetics
dc.titleMolecular phylogenetic species in Alternaria pathogens infecting pistachio and wild relatives
dc.typeArticle

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