Purification of NAD+ glycohydrolase from human serum

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dc.contributor.authorCoskun, Ozlem
dc.contributor.authorNurten, Rustem
dc.date.accessioned2025-01-27T20:53:49Z
dc.date.available2025-01-27T20:53:49Z
dc.date.issued2013
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractIn the present study, NAD(+) glycohydrolase was purified from serum samples collected from healthy individuals using ammonium sulfate fractionation, Affi-Gel blue (Cibacron Blue F3GA) affinity chromatography, Sephadex G-100 column chromatography and isoelectric focusing. The final step was followed by a second Sephadex G-100 column chromatography assay in order to remove the ampholytes from the isoelectric focusing step. In terms of enhancement of specific activity, the NAD(+) glycohydrolase protein was purified similar to 480-fold, with a yield of 1% compared with the initial serum fraction. The purified fraction appeared to be homogeneous, with a molecular weight of 39 kDa, as revealed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis, and also corresponded to the soluble (monomeric) form of surface antigen CD38.
dc.description.sponsorshipResearch Fund of the University, Fatih, Istanbul [T-1157/18062001]
dc.description.sponsorshipThe present study was supported by the Research Fund of the University, Fatih, Istanbul (project T-1157/18062001).
dc.identifier.doi10.3892/ol.2013.1335
dc.identifier.endpage231
dc.identifier.issn1792-1074
dc.identifier.issn1792-1082
dc.identifier.issue1
dc.identifier.pmid23946809
dc.identifier.scopus2-s2.0-84878276248
dc.identifier.scopusqualityQ2
dc.identifier.startpage227
dc.identifier.urihttps://doi.org/10.3892/ol.2013.1335
dc.identifier.urihttps://hdl.handle.net/20.500.12428/25851
dc.identifier.volume6
dc.identifier.wosWOS:000321079100042
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSpandidos Publ Ltd
dc.relation.ispartofOncology Letters
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WoS_20250125
dc.subjectADP-ribose
dc.subjectaffinity chromatography
dc.subjectisoelectric focusing
dc.subjectNAD glycohydrolase
dc.subjectsoluble CD38
dc.titlePurification of NAD+ glycohydrolase from human serum
dc.typeArticle

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