A novel enzyme-free FI-amperometric glucose biosensor at Cu nanoparticles modified graphite pencil electrode

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dc.authoridKarakaya, Serkan/0000-0002-6401-3295
dc.contributor.authorAyaz, Selen
dc.contributor.authorKarakaya, Serkan
dc.contributor.authorEmir, Gamze
dc.contributor.authorDilgin, Didem Giray
dc.contributor.authorDilgin, Yusuf
dc.date.accessioned2025-01-27T20:31:21Z
dc.date.available2025-01-27T20:31:21Z
dc.date.issued2020
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractIn this work, graphite pencil electrode (GPE) was modified with Cu nanoparticles (CuNPs) for enzyme-free flow injection (FI) amperometric detection of glucose. To modify electrode, CuNPs were electrodeposited onto GPE surface by recording 10 successive cyclic voltammograms (CVs) of 2.0 mM Cu(NO3)(2) in 100 mM KNO3. The electrochemical characterizations of the electrodes were realized by electrochemical impedance spectroscopy (EIS) and cyclic voltammetry techniques. The SEM images and EDX spectra of the pencil leads were recorded to investigate the surface morphologies of the electrodes. Then, the electrochemical response of both unmodified GPE and CuNPs/GPE towards electrochemical oxidation of glucose was performed by recording CVs in the supporting electrolyte consisted of 100 mM NaOH and 100 mM KCl. CVs recorded at GPE showed that the electron transfer rate of glucose is relatively slow and oxidation of glucose irreversibly observed with very low current at a high positive potential (+ 0.70 V). On the other hand, glucose oxidized at more negative potential value ( + 0.45 V) than that of unmodified GPE with a view of sharp peak at CuNPs/GPE. These results show that CuNPs/GPE exhibits excellent electrocatalytic activity and high electrochemical response towards oxidation of glucose because electron transfer rate of glucose was remarkably enhanced by modification of GPE with CuNPs. In the flow injection (FI) amperometric experiments, + 0.45 V and 2.5 mL/min were used as optimal values for applied potential (E-ap) and flow rate (f(r)), respectively. A linear calibration curve was obtained in the range from 0.10 to 400 mu M glucose with a detection limit and sensitivity of 0.04 mu M and 0.830 mu A mu M-1 cm(-2), respectively. The selectivity of glucose sensor was tested in the presence of various interferences. At last step, constructed glucose biosensor was successfully tested on some real glucose samples.
dc.description.sponsorshipCanakkale Onsekiz Mart University the Scientific Research Coordination Unit [FBA-20192743]
dc.description.sponsorshipThe authors thank the COBILTUM of Canakkale Onsekiz Mart University for surface morphological analysis of the electrodes. This work was supported by Canakkale Onsekiz Mart University the Scientific Research Coordination Unit, Project number: FBA-20192743.
dc.identifier.doi10.1016/j.microc.2019.104586
dc.identifier.issn0026-265X
dc.identifier.issn1095-9149
dc.identifier.scopus2-s2.0-85077312774
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1016/j.microc.2019.104586
dc.identifier.urihttps://hdl.handle.net/20.500.12428/23111
dc.identifier.volume154
dc.identifier.wosWOS:000527366200057
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherElsevier
dc.relation.ispartofMicrochemical Journal
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectGlucose
dc.subjectFlow injection analysis
dc.subjectCopper nanoparticles
dc.subjectGraphite pencil electrode
dc.subjectAmperometric biosensor
dc.titleA novel enzyme-free FI-amperometric glucose biosensor at Cu nanoparticles modified graphite pencil electrode
dc.typeArticle

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