A novel method for constructing an acellular 3D biomatrix from bovine spinal cord for neural tissue engineering applications

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dc.authoridARSLAN, Yavuz Emre/0000-0003-3445-1814
dc.authoridEfe Daskan, Burcu/0000-0001-7536-8115
dc.contributor.authorArslan, Yavuz Emre
dc.contributor.authorEfe, Burcu
dc.contributor.authorArslan, Tugba Sezgin
dc.date.accessioned2025-01-27T20:29:32Z
dc.date.available2025-01-27T20:29:32Z
dc.date.issued2019
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractIn this study, we aimed at generating 3-dimensional (3D) decellularized bovine spinal cord extracellular matrix-based scaffolds (3D-dCBS) for neural tissue engineering applications. Within this scope, bovine spinal cord tissue pieces were homogenized in 0.1 M NaOH and this viscous mixture was molded to attain 3D bioscaffolds. After resultant bioscaffolds were chemically crosslinked, the decellularization process was conducted with detergent, buffer, and enzyme solutions. Nuclear remnants in the native tissue and 3D-dCBS were determined with DNA content analysis and agarose gel electrophoresis. Afterward, 3D-dCBS were biochemically characterized in depth via glycosaminoglycan (GAG) content, hydroxyproline (HYP) assay, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Cellular survival of human adipose-derived mesenchymal stem cells (hAMSCs) on the 3D-dCBS for 3rd, 7th, and 10th days was assessed via MTT assay. Scaffold and cell/scaffold constructs were also evaluated with scanning electron microscopy and histochemical studies. DNA contents for native and 3D-dCBS were respectively found to be 520.76 +/- 18.11 and 28.80 +/- 0.20 ng/mg dry weight (n = 3), indicating a successful decellularization process. GAG content, HYP assay, and SDS-PAGE results proved that the extracellular matrix was substantially preserved during the decellularization process. In conclusion, it is believed that the novel decellularization method may allow fabricating 3D bioscaffolds with desired geometry from soft nervous system tissues.
dc.description.sponsorshipMinistry of Science, Industry and Technology, Republic of Turkey [0089]
dc.description.sponsorshipMinistry of Science, Industry and Technology, Republic of Turkey, Grant/Award Number: 0089. TGSD. 2013
dc.identifier.doi10.1002/btpr.2814
dc.identifier.issn8756-7938
dc.identifier.issn1520-6033
dc.identifier.issue4
dc.identifier.pmid30963718
dc.identifier.scopus2-s2.0-85064593078
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.1002/btpr.2814
dc.identifier.urihttps://hdl.handle.net/20.500.12428/22972
dc.identifier.volume35
dc.identifier.wosWOS:000481421900020
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherWiley
dc.relation.ispartofBiotechnology Progress
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectbioscaffolds
dc.subjectbovine spinal cord
dc.subjectdecellularization
dc.subjecthuman adipose-derived mesenchymal stem cells
dc.subjectneural tissue engineering
dc.titleA novel method for constructing an acellular 3D biomatrix from bovine spinal cord for neural tissue engineering applications
dc.typeArticle

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