A novel high-resolution melting (HRM)-based approach to differentiate ancient wheat species from common bread wheat via custom primer design

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dc.authorid0000-0001-5330-7442
dc.contributor.authorHurkan, Yasemin Kemec
dc.contributor.authorGocer, Emine Uygur
dc.contributor.authorHurkan, Kaan
dc.date.accessioned2026-02-03T12:03:01Z
dc.date.available2026-02-03T12:03:01Z
dc.date.issued2025
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractAncient wheat species such as Triticum monococcum L. (einkorn), Triticum dicoccum Schrank. (emmer), and Triticum spelta L. (spelt) are attracting increasing attention for their nutritional qualities, environmental adaptability, and cultural importance. However, ensuring the authenticity of these heritage grains is critical to prevent adulteration with modern bread wheat (Triticum aestivum L.), which may compromise both market value and genetic conservation. In this study, we developed and validated a high-resolution melting (HRM) assay targeting polymorphic regions of the internal transcribed spacer (ITS) locus to differentiate ancient wheat species from T. aestivum. Newly designed HRM-compatible primers generated distinct species-specific melt curve profiles, enabling reliable discrimination among the studied wheat species. The assay successfully detected admixtures containing as little as 0.2% (1/500 w/w) T. aestivum in ancient wheat flours. Principal component analysis (PCA) of HRM difference curve data enhanced resolution and revealed clear clustering patterns separating pure ancient wheat samples from adulterated ones. Additionally, ITS-based phylogenetic analysis confirmed the correct taxonomic placement of all tested species, reflecting their evolutionary relationships from diploid T. monococcum to tetraploid T. dicoccum and hexaploid T. spelta and T. aestivum. These findings demonstrate that ITS-HRM analysis offers a rapid, sensitive, and cost-effective molecular tool for authenticating ancient wheat products, supporting regulatory traceability, food labeling accuracy, and the conservation of agrobiodiversity.
dc.identifier.doi10.1007/s10722-025-02634-2
dc.identifier.issn0925-9864
dc.identifier.issn1573-5109
dc.identifier.issue1
dc.identifier.scopus2-s2.0-105024657716
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.1007/s10722-025-02634-2
dc.identifier.urihttps://hdl.handle.net/20.500.12428/34943
dc.identifier.volume73
dc.identifier.wosWOS:001637720000007
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofGenetic Resources and Crop Evolution
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WOS_20260130
dc.subjectAncient wheat authentication
dc.subjectHigh-resolution melting (HRM) analysis
dc.subjectITS barcode region
dc.subjectFood adulteration detection
dc.subjectGenetic diversity conservation
dc.subjectPrincipal component analysis (PCA)
dc.titleA novel high-resolution melting (HRM)-based approach to differentiate ancient wheat species from common bread wheat via custom primer design
dc.typeArticle

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