Effects of dietary malic acid supplementation on growth performance, antioxidant and immunological parameters, and intestinal gene expressions in rainbow trout, Oncorhynchus mykiss

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dc.authoridGhafarifarsani, Hamed/0000-0003-1199-6109
dc.authoridyousefi, morteza/0000-0001-5352-8106
dc.contributor.authorYousefi, Morteza
dc.contributor.authorGhafarifarsani, Hamed
dc.contributor.authorRaissy, Mehdi
dc.contributor.authorYılmaz, Sevdan
dc.contributor.authorVatnikov, Yury Anatolyevich
dc.contributor.authorKulikov, Evgeny Vladimirovich
dc.date.accessioned2025-01-27T20:29:49Z
dc.date.available2025-01-27T20:29:49Z
dc.date.issued2023
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractMalic acid (MA) is one of the organic acids that has been less studied for its effects on fish growth performance and gut health. The present study aimed to assess the effects of dietary MA supplementation on growth performance, antioxidant and immunological parameters, and intestinal gene expressions in rainbow trout, Oncorhynchus mykiss. Fish with similar to 25 g body weight were randomly distributed into 12 tanks at a density of 15 fish per tank and were fed with diets containing 0 (control), 0.25, 0.5, and 1% MA over 8 weeks. The results indicated that dietary MA persuaded no significant changes in growth performance, feed intake and survival rate. There were elevations in serum alanine aminotransferase (ALT; 0.5 and 1% MA), aspartate aminotransferase (AST; 0.5 and 1% MA), lysozyme (0.25, 0.5 and 1% MA), catalase (CAT; 0.5 and 1% MA), superoxide dismutase (SOD; 1% MA), glutathione pemxidase (GPx; 0.25 and 0.5% MA) activities, and total immunoglobulin (Ig) level (0.5% MA) in the fish MA-supplemented diets, compared to the control. Serum alkaline phosphatase (ALP) activity showed a decrease in 0.5% MA treatment compared to the control. Skin mucus lysozyme (0.25% MA), alternative complement activity (ACH50; 0.5% MA), and total Ig (0.25 and 0.5% MA) showed significant elevations, whereas skin mucus ALP (0.5% MA) exhibited a significant decrease in MA-treated fish, compared to the control fish. Intestinal il-1 beta (0.5 and 1% MA), hsp70 (0.5% MA), tnf-alpha (0.5% MA), tgf-beta (1% MA), caspase 3 (1% MA), and caspase 9 (1% MA) expressions significantly increased in M-treated fish, compared to the control. In conclusion, this study showed that dietary inclusion of malic acid at the rate of 0.25% in O. mykiss diets improved the antioxidant status and immune responses of fish without any negative effect on growth performance.
dc.description.sponsorshipRUDN University Scientific Projects Grant System; [202196-2-174]
dc.description.sponsorshipThis publication has been supported by the RUDN University Scientific Projects Grant System, project No 202196-2-174.
dc.identifier.doi10.1016/j.aquaculture.2022.738864
dc.identifier.issn0044-8486
dc.identifier.issn1873-5622
dc.identifier.scopus2-s2.0-85139722936
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1016/j.aquaculture.2022.738864
dc.identifier.urihttps://hdl.handle.net/20.500.12428/23045
dc.identifier.volume563
dc.identifier.wosWOS:000877656500002
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherElsevier
dc.relation.ispartofAquaculture
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectOrganic acid
dc.subjectNutrition
dc.subjectTranscriptomic
dc.subjectOxidative stress
dc.subjectImmunity
dc.titleEffects of dietary malic acid supplementation on growth performance, antioxidant and immunological parameters, and intestinal gene expressions in rainbow trout, Oncorhynchus mykiss
dc.typeArticle

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