Evaluation of hepatic and remote organ injury in an experimental liver ischemia-reperfusion model in rats and the effects of quercetin on this damage

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dc.contributor.authorAri, Neziha Senem
dc.contributor.authorYulug, Esin
dc.contributor.authorAri, Bunyamin
dc.contributor.authorKaya, Cansu
dc.contributor.authorKose, Goksen Derya Reis
dc.contributor.authorZengin, Tugba
dc.contributor.authorKeskin, Oguzhan
dc.date.accessioned2026-02-03T12:00:23Z
dc.date.available2026-02-03T12:00:23Z
dc.date.issued2025
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractBACKGROUND: This study aims to show the changes in the liver, lung, kidney, and heart in the liver ischemia-reper fusion model rats and the effect of quercetin on these changes histopathologically and immunohistochemically. METHODS: Eighteen Sprague Dawley rats were classified into three groups: Group 1 sham, Group 2 ischemia-reper fusion Group 3 ischemia-reper fusion + quercetin (IR+Q). For three days, distilled water was given to Group 1, and quercetin was given Group 3 via gavage. At the end of the third day, abdominal opening-closing was applied to Group 1, and 4 hours of reperfusion applied to Groups 2 and 3 after 1 hour of ischemia by clamping the hepatoduodenal ligament, and all rats were euthanized. Liver, kidney, and heart tissue samples were stained with Hematoxylin Eosin (HE), Masson Trichrome, Periodic Acid-Schiff (PAS), and TUNEL (Terminal deoxynucleotidyl transferase (TdT) deoxyuridine triphosphate nick end labeling assay) to assess apoptosis and examined histopathologically and immunohistochemically under a light microscope. RESULTS: In the liver, the damage score was significantly higher in the IR group than in the sham group, while it was significantly in the IR+Q group than in the IR group. While there was no significant difference between the groups in semi-quantitative scoring parameters, the Apoptotic Index was significantly higher in the IR group than in the sham group and significantly lower in the group than in the IR group. In the lung, no significant difference in lung damage scores between the groups was observed. While Apoptotic Index was significantly higher in the IR group than in the sham group, it was significantly lower in the IR+Q group than IR group. In the kidneys, tubular cell degeneration and intertubular vascular congestion were significantly higher in the IR group than the sham group. While the Apoptotic Index was higher in the IR group than in the sham and IR+Q groups, it was higher in the group than in the sham group. In the heart, there was no difference between the groups in terms of myocardial cell degeneration vascular damage. The apoptotic index was significantly higher in the IR group than in the sham and IR+Q groups. CONCLUSION: Our results indicate that histopathological damage occurs in the liver, lung, kidney, and heart in the experimentally created IR model, and quercetin application decreases IR-related damage and apoptosis in these organs.
dc.identifier.doi10.14744/tjtes.2025.61423
dc.identifier.endpage830
dc.identifier.issn1306-696X
dc.identifier.issn1307-7945
dc.identifier.issue9
dc.identifier.pmid40910710
dc.identifier.scopus2-s2.0-105015452270
dc.identifier.scopusqualityQ2
dc.identifier.startpage823
dc.identifier.urihttps://doi.org/10.14744/tjtes.2025.61423
dc.identifier.urihttps://hdl.handle.net/20.500.12428/34594
dc.identifier.volume31
dc.identifier.wosWOS:001653315500001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherTurkish Assoc Trauma Emergency Surgery
dc.relation.ispartofUlusal Travma Ve Acil Cerrahi Dergisi-Turkish Journal of Trauma & Emergency Surgery
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WOS_20260130
dc.subjectIschemia-reperfusion injury
dc.subjectliver
dc.subjectquercetin
dc.subjectremote organ
dc.titleEvaluation of hepatic and remote organ injury in an experimental liver ischemia-reperfusion model in rats and the effects of quercetin on this damage
dc.typeArticle

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