Cultured corneas show dendritic spread and restrict herpes simplex virus infection that is not observed with cultured corneal cells

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dc.authoridShukla, Deepak/0000-0002-3039-6953
dc.authoridAgelidis, Alex/0000-0002-6582-1630
dc.contributor.authorThakkar, Neel
dc.contributor.authorJaishankar, Dinesh
dc.contributor.authorAgelidis, Alex
dc.contributor.authorYadavalli, Tejabhiram
dc.contributor.authorMangano, Kyle
dc.contributor.authorPatel, Shrey
dc.contributor.authorTekin, Sati Zeynep
dc.date.accessioned2025-01-27T20:11:42Z
dc.date.available2025-01-27T20:11:42Z
dc.date.issued2017
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractHerpes simplex virus-1 (HSV-1) causes life-long morbidities in humans. While fever blisters are more common, occasionally the cornea is infected resulting in vision loss. A very intriguing aspect of HSV-1 corneal infection is that the virus spread is normally restricted to only a small fraction of cells on the corneal surface that connect with each other in a dendritic fashion. Here, to develop a comprehensive understanding of the susceptibility of human corneal epithelial (HCE) cells to HSV-1 infection, we infected HCE cells at three different dosages of HSV-1 and measured the outcomes in terms of viral entry, gene and protein expression, viral replication and cytokine induction. In cultured cells, infectivity and cytokine induction were observed even at the minimum viral dosage tested, while a more pronounced dose-restricted infectivity was seen in ex vivo cultures of porcine corneas. Use of fluorescent HSV-1 virions demonstrated a pattern of viral spread ex vivo that mimics clinical findings. We conclude that HCE cell cultures are highly susceptible to infection whereas the cultured corneas demonstrate a higher ability to restrict the infection even in the absence of systemic immune system. The restriction is helped in part by local interferon response and the unique cellular architecture of the cornea.
dc.description.sponsorshipNational Eye Institute [R01 EY024710]
dc.description.sponsorshipThis work was supported by a grant from the National Eye Institute (R01 EY024710) to D.S.
dc.identifier.doi10.1038/srep42559
dc.identifier.issn2045-2322
dc.identifier.pmid28198435
dc.identifier.scopus2-s2.0-85012969654
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1038/srep42559
dc.identifier.urihttps://hdl.handle.net/20.500.12428/20680
dc.identifier.volume7
dc.identifier.wosWOS:000394005000001
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherNature Portfolio
dc.relation.ispartofScientific Reports
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WoS_20250125
dc.subject3-O-Sulfated Heparan-Sulfate
dc.subjectEx-Vivo
dc.subjectStromal Keratitis
dc.subjectHsv-1 Infection
dc.subjectEntry
dc.subjectMechanisms
dc.subjectAcyclovir
dc.subjectResistance
dc.subjectMediators
dc.subjectViability
dc.titleCultured corneas show dendritic spread and restrict herpes simplex virus infection that is not observed with cultured corneal cells
dc.typeArticle

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