Fungal Keratitis Treatment Using Drug-Loaded Hyaluronic Acid Microgels

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dc.authoridSağbaş Suner, Selin / 0000-0002-3524-0675
dc.authoridŞahiner, Nurettin / 0000-0003-0120-530X
dc.contributor.authorAyyala, Ramesh S.
dc.contributor.authorSağbaş Suner, Selin
dc.contributor.authorBhethanabotla, Venkat R.
dc.contributor.authorŞahiner, Nurettin
dc.date.accessioned2025-01-27T20:34:47Z
dc.date.available2025-01-27T20:34:47Z
dc.date.issued2022
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractmicrogels using conjugation and encapsulation drug-loading techniques were utilized in the treatment of fungal keratitis. Natamycin (NAT) and amphotericin B (AMB) drugs were chemically linked to HA microgels by employing a chemical coupling agent to obtain conjugated (C-) HA:NAT and HA:AMB microgels. Also, these drugs were loaded into the HA microgel network during HA microgel preparation to attain encapsulated (E-) HA:NAT and HA:AMB microgels. The conjugation of drug molecules was confirmed by FT-IR spectra of bare and drugloaded HA microgels. It was determined that the AMB loading amount was about 4-fold higher for E-HA:AMB in comparison to C-HA:AMB microgels. Furthermore, the antifungal activity of drug conjugated and encapsulated HA:NAT and HA:AMB microgels was tested on Fusarium sp. and compared with the effect of bare drug molecules as control for up to 15 days of incubation time by means of the disc diffusion technique. The antifungal activity of 200 mu L at 20 mg/mL concentration of C-HA:NAT and C-HA:AMB microgels was not found to effectively inhibit Fusarium sp. growth after 1 day of incubation, whereas the same concentration of E-HA:NAT and E-HA:AMB microgels totally killed Fusarium sp. for up to 15 days. These E-HA:NAT and E-HA:AMB microgels show no cytotoxicity on the L929 fibroblast cells up to 1000 mu g/ mL concentration, whereas the free drug molecules destroy the cells even at 100 mu g/mL concentration.
dc.description.sponsorshipDepartment of Ophthalmology, College of Medicine, University of South Florida
dc.description.sponsorshipThe startup fund from the Department of Ophthalmology, College of Medicine, University of South Florida, is greatly acknowledged.
dc.identifier.doi10.1021/acsabm.2c00362
dc.identifier.endpage3815
dc.identifier.issn2576-6422
dc.identifier.issue8
dc.identifier.pmid35862018
dc.identifier.scopus2-s2.0-85136342384
dc.identifier.scopusqualityQ1
dc.identifier.startpage3806
dc.identifier.urihttps://doi.org/10.1021/acsabm.2c00362
dc.identifier.urihttps://hdl.handle.net/20.500.12428/23467
dc.identifier.volume5
dc.identifier.wosWOS:000832460700001
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherAmer Chemical Soc
dc.relation.ispartofAcs Applied Bio Materials
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectFusarium
dc.subjectfungal keratitis
dc.subjectdrug delivery system
dc.subjecthyaluronic acid
dc.subjectmicrogel
dc.titleFungal Keratitis Treatment Using Drug-Loaded Hyaluronic Acid Microgels
dc.typeArticle

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