Fabrication of an ultrasensitive and single-use graphite paper based immunosensor for Neuropeptide Y detection: A promising biosensing system for early detection of childhood obesity

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dc.contributor.authorOzcan, Burcu
dc.contributor.authorSezgintürk, Mustafa Kemal
dc.date.accessioned2025-01-27T20:17:16Z
dc.date.available2025-01-27T20:17:16Z
dc.date.issued2022
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractThe purpose of this study was to develop a label-free impedimetric immunosensing system as an analysis technique for hypothalamic Neuropeptide Y hormone with the goal of determining childhood obesity. A novel process for producing a bioelectrode modified with reduced graphene oxide and its biocompatibility with neu-ropeptide Y was discussed. The immobilization process is based on the adsorption of reduced graphene oxide (dispersed in dimethylformamide solution) onto a novel graphite paper electrode. Anti-neuropeptide Y anti-bodies were covalently immobilized on the reduced graphene oxide modified electrode surface using ethyl (dimethylaminopropyl) carbodiimide / N-Hydroxysuccinimide coupling chemistry. Electrochemical impedance spectroscopy and cyclic voltammetry techniques were used to monitor and evaluate all immobilization stages, optimization studies, and analytical characterization of the biosensor. The scanning electron microscopy was used to observe the surface morphology during the immobilization steps of the biosensor. The applicability of the designed biosensor to real serum samples was evaluated for clinical use. The biosensor demonstrated high sensitivity for determining neuropeptide Y in real serum samples. The developed immunosensor had a large linear range (0.005-10 pg/mL) and a low limit of detection for neuropeptide Y antigen (0.11 fg mL-1). Addi-tionally, the designed immunosensor demonstrated high repeatability, reproducibility, long storage stability (after the fifth week, up to 87% of initial activity), and reusability (20 times).
dc.description.sponsorshipTUEBITAK (The Scientific and Technological Research Council of Turkey) [113 Z 678]
dc.description.sponsorshipTUEBITAK (The Scientific and Technological Research Council of Turkey, (Project Number: 113 Z 678), provided financial support for this study, which is gratefully acknowledged.
dc.identifier.doi10.1016/j.mtcomm.2022.104797
dc.identifier.issn2352-4928
dc.identifier.scopus2-s2.0-85141420750
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.1016/j.mtcomm.2022.104797
dc.identifier.urihttps://hdl.handle.net/20.500.12428/21532
dc.identifier.volume33
dc.identifier.wosWOS:000897599700003
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherElsevier
dc.relation.ispartofMaterials Today Communications
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectBiosensor
dc.subjectNeuropeptide Y
dc.subjectChildhood obesity
dc.subjectGraphite paper electrode
dc.titleFabrication of an ultrasensitive and single-use graphite paper based immunosensor for Neuropeptide Y detection: A promising biosensing system for early detection of childhood obesity
dc.typeArticle

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