In vitro propagation of Silene bolanthoides Quezel, Contandr. & Pamukc. and assessment of genetic stability by flow cytometry

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dc.authoridEsen, Onur/0000-0001-8754-0293
dc.contributor.authorCorduk, Nursen
dc.contributor.authorYucel, Gulru
dc.contributor.authorAkinci, Nihan
dc.contributor.authorTuna, Metin
dc.contributor.authorEsen, Onur
dc.date.accessioned2025-01-27T21:02:06Z
dc.date.available2025-01-27T21:02:06Z
dc.date.issued2018
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractSilene bolanthoides Quezel, Contandr. & Pamukc. is an endemic species from Kazdagi (Mt. Ida), Canakkale-Balikesir, Turkey. In order to develop an efficient shoot regeneration protocol, the leaf, nodal and internodal explants of S. bolanthoides were cultured on Murashige and Skoog (MS) medium containing benzyladenine (BA) alone or in combination with alpha-naphthaleneacetic acid (NAA). The highest number of regenerated shoots (5.75 +/- 0.1) was obtained from nodal explants that were cultured on MS medium with 8.8 mu M BA+0.54 mu M NAA. Regenerated shoots were rooted on MS medium without plant growth regulators (PGRs). Rooted plants (2-3 cm) were separately transferred to pots containing a mixture of peat and perlite (3:1 v/v) and acclimatized successfully in a growth chamber. Genetic stability of the propagated plants was assessed by flow cytometry and cytological analysis. Flow cytometry analysis demonstrated that regenerated plants had 2.61 +/- 0.01 pg nuclear DNA (2C) and seed-derived plants had on average 2.58 +/- 0.02 pg/2C. Cytological analysis showed that the regenerated plants had the same chromosome number as seed-derived plants of S. bolanthoides (2n=24). It was determined that regenerated plants were uniform in chromosome number and had a similar DNA content to the seed-derived ones, indicating that the described efficient shoot regeneration protocol can be applied for ex situ conservation of this species.
dc.identifier.doi10.2298/ABS170410033C
dc.identifier.endpage148
dc.identifier.issn0354-4664
dc.identifier.issn1821-4339
dc.identifier.issue1
dc.identifier.scopus2-s2.0-85043684712
dc.identifier.scopusqualityQ3
dc.identifier.startpage141
dc.identifier.urihttps://doi.org/10.2298/ABS170410033C
dc.identifier.urihttps://hdl.handle.net/20.500.12428/27281
dc.identifier.volume70
dc.identifier.wosWOS:000428370100014
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherInst Bioloska Istrazivanja Sinisa Stankovic
dc.relation.ispartofArchives of Biological Sciences
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WoS_20250125
dc.subjectcatchfly
dc.subjectchromosome
dc.subjectendemic
dc.subjectgenetic stability
dc.subjectregeneration
dc.titleIn vitro propagation of Silene bolanthoides Quezel, Contandr. & Pamukc. and assessment of genetic stability by flow cytometry
dc.typeArticle

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