Biosensing of glucose in flow injection analysis system based on glucose oxidase-quantum dot modified pencil graphite electrode

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dc.authoridDilgin, Yusuf/0000-0002-2980-6871
dc.authoridUysal, Huseyin/0000-0003-0121-4599
dc.contributor.authorSaglam, Ozlem
dc.contributor.authorKizilkaya, Bayram
dc.contributor.authorUysal, Huseyin
dc.contributor.authorDilgin, Yusuf
dc.date.accessioned2025-01-27T20:20:15Z
dc.date.available2025-01-27T20:20:15Z
dc.date.issued2016
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractA novel amperometric glucose biosensor was proposed in flow injection analysis (FIA) system using glucose oxidase (GOD) and Quantum dot (ZnS-CdS) modified Pencil Graphite Electrode (PGE). After ZnS-CdS film was electrochemically deposited onto PGE surface, GOD was immobilized on the surface of ZnS-CdS/PGE through crosslinking with chitosan (CT). A pair of well-defined reversible redox peak of GOD was observed at GOD/CT/ZnS-CdS/PGE based on enzyme electrode by direct electron transfer between the protein and electrode. Further, obtained GOD/CT/ZnS-CdS/PGE offers a disposable, low cost, selective and sensitive electrochemical biosensing of glucose in FIA system based on the decrease of the electrocatalytic response of the reduced form of GOD to dissolved oxygen. Under optimum conditions (flow rate, 1.3 mL min(-1); transmission tubing length, 10 cm; injection volume, 100 mu L; and constant applied potential, -500 mV vs. Ag/AgCl), the proposed method displayed a linear response to glucose in the range of 0.01-1.0 mM with detection limit of 3.0 mu M. The results obtained from this study would provide the basis for further development of the biosensing using PGE based FIA systems. (C) 2015 Elsevier B.V. All rights reserved.
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK) [112T375]
dc.description.sponsorshipWe thank The Scientific and Technological Research Council of Turkey (TUBITAK) for financial support (Project number: 112T375).
dc.identifier.doi10.1016/j.talanta.2015.09.050
dc.identifier.endpage321
dc.identifier.issn0039-9140
dc.identifier.issn1873-3573
dc.identifier.pmid26592613
dc.identifier.scopus2-s2.0-84944096447
dc.identifier.scopusqualityQ1
dc.identifier.startpage315
dc.identifier.urihttps://doi.org/10.1016/j.talanta.2015.09.050
dc.identifier.urihttps://hdl.handle.net/20.500.12428/21640
dc.identifier.volume147
dc.identifier.wosWOS:000366078200045
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier Science Bv
dc.relation.ispartofTalanta
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectGlucose oxidase
dc.subjectQuantum dots
dc.subjectGlucose biosensor
dc.subjectFlow injection analysis
dc.subjectPencil graphite electrode
dc.titleBiosensing of glucose in flow injection analysis system based on glucose oxidase-quantum dot modified pencil graphite electrode
dc.typeArticle

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