A Simultaneous Analytical Method for Duplex Identification of Porcine and Horse in the Meat Products by EvaGreen based Real-time PCR

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dc.authoridAltinkaya, Emine/0000-0002-5652-3156
dc.contributor.authorSakalar, Ergun
dc.contributor.authorErgun, Seyma Ozcirak
dc.contributor.authorAkar, Emine
dc.date.accessioned2025-01-27T21:01:34Z
dc.date.available2025-01-27T21:01:34Z
dc.date.issued2015
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractA duplex real-time polymerase chain reaction (PCR) based assay for the detection of porcine and horse meat in sausages was designed by using EvaGreen fluorescent.dye. Primers were selected from mitochondrial 12S rRNA and 16S rRNA genes which are powerful regions for identification of horse and porcine meat. DNA from reference samples and industrial products was successfully extracted using the GI-DAGEN Multi-Fast DNA Isolation Kit. Genomes were identified based on their specific melting peaks (Mp) which are 82.5 degrees C and 78 degrees C for horse and porcine, respectively. The assay used in this study allowed the detection of as little as 0.0001% level of horse meat and 0.001% level of porcine meat in the experimental admixtures. These findings indicate that EvaGreen based duplex realtime PCR is a potentially sensitive, reliable, rapid and accurate assay for the detection of meat species adulterated with porcine and horse meats.
dc.identifier.doi10.5851/kosfa.2015.35.3.382
dc.identifier.endpage388
dc.identifier.issn1225-8563
dc.identifier.issue3
dc.identifier.pmid26761852
dc.identifier.scopus2-s2.0-84941644774
dc.identifier.scopusqualityN/A
dc.identifier.startpage382
dc.identifier.urihttps://doi.org/10.5851/kosfa.2015.35.3.382
dc.identifier.urihttps://hdl.handle.net/20.500.12428/27098
dc.identifier.volume35
dc.identifier.wosWOS:000357358000013
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherKorean Soc Food Science Animal Resources
dc.relation.ispartofKorean Journal For Food Science of Animal Resources
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WoS_20250125
dc.subjectduplex real-time PCR
dc.subjectEvaGreen
dc.subjecthorse
dc.subjectporcine
dc.subjectunspecific amplicon
dc.titleA Simultaneous Analytical Method for Duplex Identification of Porcine and Horse in the Meat Products by EvaGreen based Real-time PCR
dc.typeArticle

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