Quercetin protects the retina by reducing apoptosis due to ischemia-reperfusion injury in a rat model

Basit Öğe Kaydı
dc.authoridKARACA, Turan/0000-0002-2500-7781
dc.contributor.authorArikan, Sedat
dc.contributor.authorErsan, Ismail
dc.contributor.authorKaraca, Turan
dc.contributor.authorKara, Selcuk
dc.contributor.authorGencer, Baran
dc.contributor.authorKaraboga, Ihsan
dc.contributor.authorTufan, Hasan Ali
dc.date.accessioned2025-01-27T20:49:44Z
dc.date.available2025-01-27T20:49:44Z
dc.date.issued2015
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractPurpose: This study aimed to investigate the effect of quercetin on apoptotic cell death induced by ischemia-reperfusion (I/R) injury in the rat retina. Methods: Twenty-four rats were divided into four equal groups: control, ischemic, solvent, and quercetin. I/R injury was achieved by elevating the intraocular pressure above the perfusion pressure. Intraperitoneal injections of 20 mg/kg of quercetin and dimethyl sulfoxide (DMSO) were performed in the quercetin and solvent groups, respectively, immediately prior to I/R injury, and the researchers allowed for the retinas to be reperfused. Forty-eight hours after injury, the thicknesses of the retinal ganglion cell layer (RGCL), inner nuclear layer (INL), inner plexiform layer (IPL), outer plexiform layer (OPL), and outer nuclear layer (ONL) were measured in all groups. Moreover, the numbers of terminal deoxynucleotidyl transferase dUTP nick-end-labeled [TUNEL (+)] cells and caspase-3 (+) cells in both INL and ONL were evaluated in all groups. Results: The administration of quercetin was found to reduce the thinning of all retinal layers. The mean thickness of INL in the quercetin and ischemic groups was 21 +/- 5.6 mu m and 16 +/- 6.4 mu m, respectively (P<0.05). Similarly, the mean thickness of ONL in the quercetin and ischemic groups was 50 +/- 12.8 mu m and 40 +/- 8.7 mu m, respectively (P<0.05). The antiapoptotic effect of quercetin in terms of reducing the numbers of both TUNEL (+) cells and caspase-3 (+) cells was significant in INL. The mean number of TUNEL (+) cells in INL in the ischemic and quercetin groups was 476.8 +/- 45.6/mm(2) and 238.72 +/- 251/mm(2), respectively (P<0.005). The mean number of caspase-3 (+) cells in INL of ischemic and quercetin groups was 633.6 +/- 38.7/mm(2) and 342.4 +/- 36.1/mm(2), respectively (P<0.001). Conclusion: The use of quercetin may be beneficial in the treatment of retinal I/R injury because of its antiapoptotic effect on the retinal layers, particularly in INL.
dc.identifier.doi10.5935/0004-2749.20150026
dc.identifier.endpage104
dc.identifier.issn0004-2749
dc.identifier.issn1678-2925
dc.identifier.issue2
dc.identifier.pmid25945531
dc.identifier.scopus2-s2.0-84942693943
dc.identifier.scopusqualityQ3
dc.identifier.startpage100
dc.identifier.urihttps://doi.org/10.5935/0004-2749.20150026
dc.identifier.urihttps://hdl.handle.net/20.500.12428/25293
dc.identifier.volume78
dc.identifier.wosWOS:000354050800009
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherConsel Brasil Oftalmologia
dc.relation.ispartofArquivos Brasileiros De Oftalmologia
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WoS_20250125
dc.subjectQuercetin
dc.subjectRetina/drug effects
dc.subjectReperfusion injury
dc.subjectApoptosis
dc.subjectIschemia
dc.subjectDisease models
dc.subjectRats
dc.titleQuercetin protects the retina by reducing apoptosis due to ischemia-reperfusion injury in a rat model
dc.typeArticle

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu