A High Sensitive, Reproducible and Disposable Immunosensor for Analysis of SOX2

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dc.authoridTarimeri, Nur/0000-0002-7464-9107
dc.contributor.authorTarimeri, Nur
dc.contributor.authorSezgintürk, Mustafa Kemal
dc.date.accessioned2025-01-27T21:07:49Z
dc.date.available2025-01-27T21:07:49Z
dc.date.issued2020
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractIn this study, an ITO (indium tin oxide) based biosensor was constructed to detect SOX2. SOX2 helps the regulation of cell pluripotency and is closely related to early embryonic development, neural and sexual differentiation. SOX2 is amplified and overexpressed in some malignant tumors such as squamous cell, lung, prostate, breast, esophageal cell, colon, ovarian, glioblastoma, pancreatic cancer, gastric cancer, head and neck squamous cell carcinoma. To generate a hydroxylated clean electrode surface, ITO electrodes were treated with NH4OH/H2O2/H2O. Later, ITO-PET electrode surfaces were modified with 3-glycidoxypropyl trimethoxysilane (3-GOPS). Then, Anti-SOX2 was covalently immobilized onto the electrode surfaces. 3-GOPS concentration, Anti-SOX2 concentration and incubation time, SOX2 incubation time were optimized. Electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) were utilized in order to follow up the immobilization processes and the optimization steps of the biosensor. To characterize the analytical properties of constructed immunosensor; linear range, repeatability, reproducibility and regeneration studies were investigated. The linear range of the immunosensor was detected as 0.625 pg/mL-62.5 pg/mL. Square wave voltammetry technique was also applied to the biosensor. Storage life of the biosensor was determined for identifying the possible usability of the biosensor in clinical field. Finally, the designed biosensor was applied to the real human serum samples. The results obtained with the presented biosensor were also compared with ELISA results.
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK)
dc.description.sponsorshipAcknowledgments that help the COBILTUM for SEM and EDX experiments. This work was supported by the Scientific and Technological Research Council of Turkey (TUBITAK).
dc.identifier.doi10.1002/elan.201900557
dc.identifier.endpage1074
dc.identifier.issn1040-0397
dc.identifier.issn1521-4109
dc.identifier.issue5
dc.identifier.scopus2-s2.0-85078677320
dc.identifier.scopusqualityQ2
dc.identifier.startpage1065
dc.identifier.urihttps://doi.org/10.1002/elan.201900557
dc.identifier.urihttps://hdl.handle.net/20.500.12428/28188
dc.identifier.volume32
dc.identifier.wosWOS:000508461600001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherWiley-V C H Verlag Gmbh
dc.relation.ispartofElectroanalysis
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectSOX2
dc.subjectBiosensor
dc.subjectElectrochemical Impedance Spectroscopy
dc.subjectITO-PET
dc.titleA High Sensitive, Reproducible and Disposable Immunosensor for Analysis of SOX2
dc.typeArticle

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