A comparative study of the antimicrobial properties and antioxidant enzyme activities of field-grown and in vitro-propagated plants of endemic Digitalis trojana Ivanina

Basit Öğe Kaydı
dc.contributor.authorCorduk, Nursen
dc.contributor.authorDemirbas, Sefer
dc.contributor.authorDogru, Nurcihan Hacioglu
dc.date.accessioned2025-01-27T20:14:11Z
dc.date.available2025-01-27T20:14:11Z
dc.date.issued2017
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractThe antimicrobial properties and antioxidant enzyme activities of field-grown and in vitro-propagated plants of Digitalis trojana Ivanina (Helen of Troy foxglove), a perennial endemic plant species of Turkey, were compared. The field work was carried out in May and July 2014, and plant samples of D. trojana were collected from Kazdagi (Turkey). Propagation of D. trojana was achieved by culturing leaf explants on MS medium supplemented with 13.3 mu M 6-benzyl-aminopurine (BA) and 0.53 mu M alpha-naphthaleneacetic acid (NAA). The antimicrobial activity, plant lipid peroxidation levels and antioxidant enzyme (superoxide dismutase (SOD), catalase (CAT), peroxidase (POX), ascorbate peroxidase (APX), glutathione reductase (GR)) activities were analyzed in 12- and 17-week-old in vitro-grown Digitalis plants collected in May and July from two different localities at 430 and 1173 m above sea level. Although the in vitro-propagated plants had very low antagonistic activities compared to field-grown plants, they exhibited remarkably similar antibacterial activities against Escherichia coli ATCC 11230, Pseudomonas aeruginosa ATCC 27853 and Bacillus subtilis ATCC 6633. There were no important differences between plants collected from the two localities (430 and 1173 m a. s. l.). Biochemical analysis showed that the antioxidant enzyme (SOD, APX, GR) activities of field-grown plants were higher than in vitro-grown plants. Also, the difference in altitude at which the plants were grown was apparently linked to decreases in antioxidant enzyme activities, except for POX in field-grown plants collected in July.
dc.description.sponsorshipCanakkale Onsekiz Mart University Scientific Research Projects Coordination Unit, Turkey [FBA-2014-128]
dc.description.sponsorshipThis research was financially supported by the Canakkale Onsekiz Mart University Scientific Research Projects Coordination Unit, Turkey (FBA-2014-128).
dc.identifier.doi10.2298/ABS161216004C
dc.identifier.endpage610
dc.identifier.issn0354-4664
dc.identifier.issn1821-4339
dc.identifier.issue4
dc.identifier.scopus2-s2.0-85031702671
dc.identifier.scopusqualityQ3
dc.identifier.startpage603
dc.identifier.urihttps://doi.org/10.2298/ABS161216004C
dc.identifier.urihttps://hdl.handle.net/20.500.12428/20982
dc.identifier.volume69
dc.identifier.wosWOS:000413353300004
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherInst Bioloska Istrazivanja Sinisa Stankovic
dc.relation.ispartofArchives of Biological Sciences
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WoS_20250125
dc.subjectDigitalis trojana
dc.subjectin vitro propagation
dc.subjectfield-grown
dc.subjectantimicrobial activity
dc.subjectantioxidant enzyme activities
dc.titleA comparative study of the antimicrobial properties and antioxidant enzyme activities of field-grown and in vitro-propagated plants of endemic Digitalis trojana Ivanina
dc.typeArticle

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu