Genome-wide identification and in silico expression analysis of Na plus /H plus antiporter (NHX) gene family in common bean

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dc.contributor.authorSari, Ugur
dc.date.accessioned2026-02-03T12:03:02Z
dc.date.available2026-02-03T12:03:02Z
dc.date.issued2025
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractPlant Na+/H+antiporters (NHX) genes are essential Na+ transporters and play vital roles in plant growth, development, and abiotic stress responses, particularly salinity stress. However, available information on NHX genes in the common bean (Phaseolus vulgaris L.) genome is limited. In this study, a comprehensive analysis of the characteristics of the NHX genes in common bean was carried out at the whole genome level. A total of seven PvNHX genes were identified, distributed on chromosomes 2, 3, 6 and 7. Phylogenetic analysis revealed that the PvNHX genes were divided into three subgroups, namely PvNHX1, - 2, - 3, - 4 on vacuolar membranes, PvNHX5 and PvNHX6 on the endosomal region and PvNHX7 on the plasma membrane. Comparative syntenic analysis revealed that the PvNHX1, - 2, - 3, - 4, - 7 genes were colinear with 7 and 5 NHX genes in the Arabidopsis and tomato genomes, respectively. Ka/Ks analysis indicated that all duplicated PvNHX gene pairs were under the pressure of purifying selection during evolution. The amiloride binding site, a characteristic domain of NHX proteins, was absent in the PvNHX7 protein, whereas it is partially or fully conserved in all other PvNHX members. Promoter analysis showed that all NHX genes, except the PvNHX4 gene, possess ABRE (ABA-responsive) elements. The protein-protein interaction analysis indicated that the PvNHX7 protein physically interacts with CIPK and PARP proteins. Transcriptome analysis revealed that all NHX genes was up regulated in root tissues of susceptible cultivar under salt stress. In addition, the PvNHX2 gene was also considerably induced by salt stress in root and leaf tissues of the tolerant cultivar. In conclusion, this study is the first to report comprehensive and systematic genome-wide information on the NHX gene family in the common bean genome.
dc.description.sponsorshipCanakkale Onsekiz Mart University Scientific Research Projects Commission [FHD-2020-3456]
dc.description.sponsorshipThis study was supported by Canakkale Onsekiz Mart University Scientific Research Projects Commission with project number FHD-2020-3456.
dc.identifier.doi10.1007/s10722-025-02605-7
dc.identifier.endpage298
dc.identifier.issn0925-9864
dc.identifier.issn1573-5109
dc.identifier.issueSUPPL1
dc.identifier.scopus2-s2.0-105013805163
dc.identifier.scopusqualityQ2
dc.identifier.startpage281
dc.identifier.urihttps://doi.org/10.1007/s10722-025-02605-7
dc.identifier.urihttps://hdl.handle.net/20.500.12428/34944
dc.identifier.volume72
dc.identifier.wosWOS:001555767500001
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthorSari, Ugur
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofGenetic Resources and Crop Evolution
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WOS_20260130
dc.subjectPhaseolus vulgaris L.
dc.subjectNa(+)/H(+)Antiporter
dc.subjectNHX
dc.subjectSalt stress
dc.subjectGene expression
dc.titleGenome-wide identification and in silico expression analysis of Na plus /H plus antiporter (NHX) gene family in common bean
dc.typeArticle

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