The Effectiveness of Temperature Versus Aprotinin in Maintaining the Preanalytical Stability of Adrenocorticotrophin

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dc.contributor.authorTurkon, Hakan
dc.contributor.authorToprak, Burak
dc.contributor.authorYalcin, Hulya
dc.contributor.authorColak, Ayfer
dc.contributor.authorOzturk, Nurinnisa
dc.date.accessioned2025-01-27T20:38:36Z
dc.date.available2025-01-27T20:38:36Z
dc.date.issued2016
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractObjective: To investigate the effect of temperature and protease inhibitor on adrenocorticotrophin (ACTH) stability. Methods: We collected blood specimens from 10 donors into K3 EDTA (ethylenediaminetetraacetic acid) and K3 EDTA + aprotinin tubes. Plasma specimens were held at room temperature and at 2 degrees C to 8 degrees C for 0, 2, 4, 8, 24, and 72 hours. ACTH concentrations in 4 storage conditions were compared with repeated-measures analysis of variance (ANOVA) at each time point. Results: ACTH concentrations substantially decreased after a 72-hour time period. At the 72nd hour, standard EDTA tubes and EDTA + aprotinin tubes stored at 4 degrees C showed the optimal stability. EDTA + aprotinin tubes stored at room temperature performed more strongly than standard EDTA tubes stored at room temperature. Conclusion: Our results show that refrigerated storage is more effective than protease inhibitor addition to maintain ACTH stability. We recommend using aprotinin tubes, especially for specimens standing at room temperature.
dc.identifier.doi10.1093/labmed/lmw027
dc.identifier.endpage282
dc.identifier.issn0007-5027
dc.identifier.issn1943-7730
dc.identifier.issue4
dc.identifier.pmid27593170
dc.identifier.scopus2-s2.0-85040939448
dc.identifier.scopusqualityQ3
dc.identifier.startpage279
dc.identifier.urihttps://doi.org/10.1093/labmed/lmw027
dc.identifier.urihttps://hdl.handle.net/20.500.12428/23664
dc.identifier.volume47
dc.identifier.wosWOS:000388610900005
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherOxford Univ Press
dc.relation.ispartofLaboratory Medicine
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WoS_20250125
dc.subjectACTH
dc.subjectaprotinin
dc.subjectstability
dc.subjectproteases
dc.subjectpreanalytical phase
dc.subjecttemperature
dc.titleThe Effectiveness of Temperature Versus Aprotinin in Maintaining the Preanalytical Stability of Adrenocorticotrophin
dc.typeArticle

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