Yazar "Ozyurt, Canan" seçeneğine göre listele

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    Fluid-based wearable sensors: a turning point in personalized healthcare
    (Tubitak Scientific & Technological Research Council Turkey, 2023) Vural, Berin; Uludag, Inci; Ince, Bahar; Ozyurt, Canan; Ozturk, Funda; Sezgintürk, Mustafa Kemal
    Nowadays, it has become very popular to develop wearable devices that can monitor biomarkers to analyze the health status of the human body more comprehensively and accurately. Wearable sensors, specially designed for home care services, show great promise with their ease of use, especially during pandemic periods. Scientists have conducted many innovative studies on new wearable sensors that can noninvasively and simultaneously monitor biochemical indicators in body fluids for disease prediction, diagnosis, and management. Using noninvasive electrochemical sensors, biomarkers can be detected in tears, saliva, perspiration, and skin interstitial fluid (ISF). In this review, biofluids used for noninvasive wearable sensor detection under four main headings, saliva, sweat, tears, and ISF-based wearable sensors, were examined in detail. This report analyzes nearly 50 recent articles from 2017 to 2023. Based on current research, this review also discusses the evolution of wearable sensors, potential implementation challenges, and future prospects.
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    Lateral flow assays for food analyses: Food contaminants, allergens, toxins, and beyond
    (Elsevier Sci Ltd, 2023) Ince, Bahar; Uludag, Inci; Demirbakan, Burcak; Ozyurt, Canan; Ozcan, Burcu; Sezgintürk, Mustafa Kemal
    Food safety is a major issue because of the prevalence of pathogens, chemicals, and other potentially harmful substances in food products. Because of this, the creation of a reliable and accessible tool for checking on food quality is essential. In comparison to traditional techniques, lateral flow tests for the identification of food-borne pathogens are more modern, straightforward, and quick. This review examines the use of lateral flow biosensors for the detection of various food contaminants and the labels used to improve the effectiveness of the system. Since LFAs may be carried out by non-specialists, they are well-suited for on-site testing in a variety of food production and distribution environments.
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    MerR-fluorescent protein chimera biosensor for fast and sensitive detection of Hg2+ in drinking water
    (Wiley, 2019) Ozyurt, Canan; Ustukarci, Handan; Evran, Serap; Telefoncu, Azmi
    Mercury ion (Hg2+) is a universal pollutant and its detection is crucial for public healthcare. In this study, we developed a novel fluorescent biosensor by construction of a protein fusion between the mercury-sensing transcription factor MerR and enhanced yellow fluorescent protein (EYFP). Hg2+-induced conformational change of MerR was transduced into fluorescence signal. Fluorescence intensity of the biosensor protein decreased with increasing concentrations of Hg2+ and a linear response was obtained in the range of 0.5-40 nM. The limit of detection was 0.5 nM, which was much lower than the maximum allowed level in water. The biosensor specificity was highly dependent on type and concentration of metal ion. The biosensor exhibited high specificity in a mixture of metal ions at 0.5 nM concentration. However, the interference effect was more pronounced at 40 nM concentration of metal ions. The measurement was completed in less than 1 Min with no need for sample preparation or preincubation steps. The biosensor achieved accurate and reliable detection in the spiked drinking water sample, as validated by the inductively coupled plasma optical emission spectrometry.
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    Single-stranded DNA (ssDNA) Aptamer targeting SipA protein inhibits Salmonella Enteritidis invasion of intestinal epithelial cells
    (Elsevier, 2020) Shatila, Fatima; Yalcin, H. Tansel; Ozyurt, Canan; Evran, Serap; Cakir, Busra; Yasa, Ihsan; Nalbantsoy, Ayse
    Salmonella Enteritidis is an important pathogen that can invade the intestinal cells of its host causing salmonellosis. SipA protein, an effector protein secreted by T3SS, maintains invasion of host cells more efficient. Thus, inhibitory aptamers against SipA protein were developed using magnetic bead-based Systematic Evolution of Ligands by Exponential Enrichment (SELEX) method. The enriched sequences were obtained after 9 SELEX rounds. Among which, an aptamer namely Apt17 displayed Kd values equivalent to 114.9 and 63.4 nM at 27 degrees C and 37 degrees C, respectively. The effect of Apt17 on adhesion and invasion of Caco-2 cells by the tested strains was determined. While the adhesion and invasion of Salmonella Enteritidis TM 6 were inhibited by 70% and 37.7%, those of Salmonella Enteritidis TM 68 were inhibited by 45.71% and 39.5% respectively. These results represent a corner stone for future studies that could aim to develop putative inhibitors against Salmonellosis. (C) 2020 Elsevier B.V. All rights reserved.
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    The single-step synthesis of thiol-functionalized phosphazene-based polymeric microspheres as drug carrier
    (Taylor & Francis Inc, 2020) Ozay, Hava; Ilgin, Pinar; Ozyurt, Canan; Özay, Özgür
    Herein, new microspheres were successfully fabricated from pentaerythritol tetrakis(3-mercaptopropionate) as model monomer and hexachlorocyclotriphosphazene as crosslinker using a single-step via precipitation polymerization method. Trimethoprim was chosen as the model antibiotic drug for drug release and antimicrobial activity studies. The percent of drug loading and drug entrapment efficiency of the microspheres was calculated to be 0.85% and 42.2%. According to correlation coefficients, the release kinetic was found to be Higuchi kinetic model and the release mechanism was non-Fickian model for microspheres. The drug-loaded microspheres exhibited excellent bacterial inhibition against gram-positive (Staphylococcus aureusandBacillus subtilis) and gram-negative (Escherichia coli) bacteria.