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A Schiff base colorimetric probe for real-time naked-eye detection of biologically important fluoride and cyanide ions: Single crystal, experimental, theoretical, biological and antioxidant studies
(Elsevier, 2020) Unver, Huseyin; Boyacioglu, Bahadir; Demir, Neslihan; Zeyrek, Celal Tugrul; Yıldız, Mustafa
The synthesis and characterization of a Schiff base (E)-2-((3,5-bis(trifluoromethyl)phenylimino)methyl)4-methylphenol from the reaction of 2-hydroxy-5-methylbenzaldehyde with 3,5-bis(trifluoromethyl) aniline has been studied using X-ray single-crystal techniques and compared to the results from theoretical calculations using density functional theory (DFT). We have investigated the antimicrobial activities of the title compound for their minimum inhibitory concentration (MIC). We have also examined the interactions with DNA by using the UV-Visible and agarose gel electrophoresis methods. It is shown that the title compound interacts with CT-DNA via electrostatic binding. The antioxidant activity of the title compound was measured by using the DPPH method. It is furthermore shown that it indicates lower antioxidant activity than butylated hydroxytoluene (BHT). It is also found that activity remained constant with increasing compound concentration. We have also investigated the colorimetric response of the title compound in dimethyl sulfoxide (DMSO) to the addition of an equivalent amount of anions (F-, Br-, I-, CN-, SCN-, ClO4-, HSO4-, AcO-, H2PO4 and OH-). In this context, while the addition of Fand CN anions into the solution containing the title compound resulted in a significant colour change, the addition of Br-, I-, SCN-, ClO4-, HSO4-, AcO-, H2PO4 and OH anions resulted in no colour change. In the presence of these anion solutions, only Fand CN solutions underwent impressive colour change detectable with the naked eye from colourless to orange-red and purple, and orange and blue-violet in both daylight and UV light, respectively. The most discernible colour change in the Schiff base was caused by Fand CN-, which demonstrated that the Schiff base could be used to selectively detect Fand CN-. Also, various sensor applications of Schiff base were investigated. For this, the alumina oxide sheet and filter paper impregnated with the title compound were used. (C) 2020 Elsevier B.V. All rights reserved.
Anti-bacterial, anti-oxidant activities of polymer-based metal complexes and their catalyst effects in presence of H2O2
(Springer Science and Business Media Deutschland GmbH, 2024) Şenol Bahçeci, Dilek; Aydın Kocaeren, Aysel; Demir, Neslihan; Dalgıç, Büşra
4-{[(3-Hydroxynaphthalen-2-yl)imino]methyl}benzene-1,2,3-triol Schiff-base, abbreviated as ligand (L), was synthesized and additionally, its polymer was obtained through oxidative polycondensation and named as poly-4-{[(3-hydroxynaphthalen-2-yl)imino]methyl}benzene-1,2,3-triolpoly-ligand (PL). The ammonium persulfate (NH4)2S(2)O(8) was used as an oxidant in this reaction. The metal complexes of PL were synthesized in the presence of various metal ions such as Cu2+, Hg2+, Ni2+, Pb2+,and Zn2+. The synthesized structures of PL and metal complexes were confirmed by FTIR, UV-vis, H-1 and C-13 NMR, and elemental analysis. Moreover, SEM and TGA analyses were performed for characterization. In this study, the anti-oxidant, anti-bacterial and catalytic properties of PL and its metal complexes were investigated. For anti-oxidant properties, 2,2-diphenyl-1-picryl-hydrazyl (DPPH) detection methods were studied. Also, for anti-bacterial activities they were tested against various bacteria using the minimum inhibitory concentration (MIC) method. It was observed that [Ni(PL)(2)] and [Pb(PL)(2)] metal complexes showed quite good anti-oxidant activities when they were compared with PL at its highest concentration of 100 ppm. The anti-bacterial activity results showed that [Hg(PL)(2)] had the highest MIC value of all the mentioned materials. The obtained catalytic activity results revealed that the synthesized poly-ligand metal [M(PL)(2)] complexes could be considered as an alternative catalyst to remove various organic pollutants from the aqueous environment. From this point of view, it is possible to say that [M(PL)(2)] complexes synthesized within the scope of the study could be used as polymeric agents to reduce environmental pollution.
Assessment of pollution biomarker and stable isotope data in Mytilus galloprovincialis tissues
(Springer, 2019) Yalçın Özdilek, Şükran; Demir, Neslihan; Gurkan, Selin Erturk
Superoxide dismutase (SOD) is one of the antioxidant defense enzymes in mussels and converts the superoxide anion into hydrogen peroxide and this enzyme is used as biomarkers of oxidative damage. As well as many topics in ecology, stable isotopes are also signature for organic and heavy metal pollution in aquatic ecosystems. This study aims to compare the stable carbon and nitrogen values of different mussel tissues and the changes on the SOD values of the same tissues in order to understand the relationship between two mechanisms of bioindicator processes of physiological response of mussel to pollution. The changes in SOD activity in the gill, hepatopancreas, and mantle tissues of Mytilus galloprovincialis with C-13 and N-15 isotopes were assessed in two locations (Kepez and Guzelyal) in Canakkale. The SOD values of mussel samples were found as the gill > hepatopancreas > mantle collected from Kepez and the gill > hepatopancreas collected from Guzelyal. There were no significant differences among the mean SOD values of different tissues. There was enrichment both in nitrogen and carbon isotope values of hepatopancreas tissues both in Kepez and Guzelyal samples. There was a negative correlation between both isotope values and SOD values of samples. As well as SOD values, the isotopic composition of particularly hepatopancreas tissue is a good indicator for evaluation of pollution.
Biological Activity and Optical Sensor Properties of Green Synthesis Polymer
(Wiley-V C H Verlag Gmbh, 2022) Bahceci, Dilek Senol; Demir, Neslihan; Kocaeren, Aysel Aydin
In this study, 4-amino-3-hydroxy-1-naphthalene sulfonic acid (Monomer) was converted to its enzyme-catalyzed oxidative polymerization (Poly-enz) in dioxane solvent by horseradish peroxidase enzyme (HRP) in the presence of hydrogen peroxide as oxidant. For the analysis of the monomer and its synthesized Poly-enz, H-1-NMR, C-13-NMR, FT-IR, TGA, and SEM measurements were determined. In addition, the optical ion sensor properties of Poly-enz against metals such as Ag+, Cd2+, Co2+, Hg2+, Ni2+, Pb2+, Zn2+, Cu2+ were examined with UV-Vis spectrophotometer. It was observed that Cu metal showed sensory properties. DNA cleavage and DNA binding activities of monomer and Poly-enz were done using by agarose gel electrophoresis and UV-Vis methods, respectively. It was seen that the Poly-enz interacted with DNA electrostatically. This enzymatic polymer was found to cleave pBR322 plasmid DNA both hydrolytically and oxidatively as well. The antioxidant activities of the compounds were examined by three different methods (DPPH, ABTS, FRAP) and it was found that the Poly-enz showed high antioxidant activity compared to its monomer. Antimicrobial activities were studied against various bacterial and yeast cultures by the minimum inhibition concentration (MIC) method. It was observed that the antimicrobial activities of the enzymatic polymer in different microorganisms increased depending on the concentration.
CAN GANODERMA LUCIDUM BE AN ALTERNATIVE NUTRITIONAL SUPPLEMENT FOR ENHANCING SPERM MOTILITY RATE?
(İzmir Demokrasi Üniversitesi, 2021) Büyük, Başak; Demirci, Tuba; Demir, Neslihan; Türkön, Hakan
Ganoderma lucidum (GL) is a widely used medicinal mushroom. The therapeutic effect of this fungus on many diseases has been proven by studies. The aim of this study is to assess the effects of low, moderate and high dose GL extract administration on the testis tissue, spermatogenic series cells and sperm motility in rats. 40 Wistar albino rats were randomly divided into 4 groups. Rats in group 1, 2, 3 and 4 were administered 2 ml physiologic serum, 500 mg/kg, 2500 mg/kg, 5000 mg/kg Ganoderma lucidum extract 1 time per day via gavage for 9 days, respectively. For evaluation of sperm motility and histopathological changes, epididymal sperm collection and testis harvesting were done. Blood samples were collected for biochemical analysis. When the Group 1 (control group) is compared with Groups 2, 3 and 4, the Johnsen score and sperm motility in these groups increased and this increase was statistically significant. In conclusion, low, moderate, and high doses of GL extract administered to rats were revealed to increase spermatogenesis, epididymal total sperm count and progressive motile sperm counts. However, it is detected that high doses cause minimal damage to the testis and as the increase in sperm parameters wasn’t significant, it’s concluded that doses for oral use above 2500 mg/kg should be avoided
Çanakkale Boğazı (Kepez) Midye (Mytilus galloprovincialis L., 1819) Örneklerinde Ağır Metal ve Antioksidan Enzim Düzeylerinin Mevsimsel Değişimi
(2018) Demir, Neslihan; Akkuş, Gülsüm
Bu çalışmada, yoğun bir deniz trafiğine sahip olan Çanakkale Boğazı Kepez kıyılarındaki ağır metal kirliliğinin midyeler üzerindeki etkileri araştırıldı. Bu amaçla, Akdeniz Midyesi (Mytilus galloprovincialis) solungaç, hepatopankreas ve kas dokularında toplam protein (TP), süperoksit dismutaz (SOD) ve katalaz (CAT) düzeylerindeki değişimler incelendi. Mevsimsel farklılıkları değerlendirebilmek için dokulardaki kadmiyum (Cd), bakır (Cu), demir (Fe), kurşun (Pb) ve çinko (Zn) ağır metallerinin birikim düzeyleri de tayin edildi. M. galloprovincialis örneklerinin solungaç, hepatopankreas ve kas dokularındaki ağır metal miktarlarının (sonbaharda hepatopankreasta ölçülen Pb değeri hariç) kabul edilebilir değerlerin altında olduğu belirlendi. TP miktarı, en yüksek hepatopankreas dokusunda sonbahar mevsiminde görüldü. SOD aktivitesi, ilkbahar mevsiminde daha yüksek görülmekle birlikte solungaç ve kas dokularında mevsimsel olarak anlamlı farklılık gözlendi (p<0.05). CAT aktivitesinde solungaç ve hepatopankreas dokularında sonbaharda ilkbahara oranla belirgin ve anlamlı bir düşüş görüldü. Belirlenen farklılıklar, antioksidatif savunma mekanizmalarının ağır metal konsantrasyonlarıyla güçlü bir korelasyon gösterdiğini ve sucul kirliliğin belirlenmesi ve engellenmesinde enzimatik aktivitenin en genel belirteç olduğunu ortaya koydu.
Characterization, biological activity, and anticancer effect of green-synthesized gold nanoparticles using Nasturtium officinale L.
(BMC, 2024) Yayintas, Ozlem Tonguc; Demir, Neslihan; Canbolat, Fadime; Ayna, Tuelay Kilicaslan; Pehlivan, Melek
Background Nanostructured materials used have unique properties and many uses in nanotechnology. The most striking of these is using herbal compounds for the green synthesis of nanoparticles. Among the nanoparticle types used for green synthesis, gold nanoparticles (AuNPs) are used for cancer therapy due to their stable structure and non-cytotoxic. Lung cancer is the most common and most dangerous cancer worldwide in terms of survival and prognosis. In this study, Nasturtium officinale (L.) extract (NO), which contains biomolecules with antioxidant and anticancer effects, was used to biosynthesize AuNPs, and after their characterization, the effect of the green-synthesized AuNPs against lung cancer was evaluated in vitro. Methods Ultraviolet-visible (UV-Vis) spectrophotometry, scanning electron microscopy (SEM), transmission electron microscopy (TEM), energy dispersive X-ray spectroscopy (EDS), multiple analysis platform (MAP), and Fourier transform infrared (FT-IR) spectroscopy analyses were performed to characterize the AuNPs prepared from the N. officinale plant extract. Moreover, the antioxidant activity, total phenolic and flavonoid contents and DNA interactions were examined. Additionally, A549 lung cancer cells were treated with 2-48 mu g/mL Nasturtium officinale gold nanoparticles (NOAuNPs) for 24 and 48 h to determine the effects on cell viability. The toxicity of the synthesized NOAuNPs to lung cancer cells was determined by the 3-(4,5-dimethylthiazol-2-il)-2,5-diphenyltetrazolium bromide (MTT) assay, and the anticancer effect of the NOAuNPs was evaluated by apoptosis and cell cycle analyses using flow cytometry. Results The average size of the NPs was 56.4 nm. The intensities of the Au peaks from EDS analysis indicated that the AuNPs were synthesized successfully. Moreover, the in vitro antioxidant activities of the NO and NOAuNPs were evaluated; these materials gave values of 31.78 +/- 1.71% and 31.62 +/- 0.46%, respectively, in the 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay at 200 g/mL and values of 25.89 +/- 1.90% and 33.81 +/- 0.62%, respectively, in the 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assay. The NO and NOAuNPs gave values of 0.389 +/- 0.027 and 0.308 +/- 0.005, respectively, in the ferrous ion reducing antioxidant capacity (FRAP) assay and values of 0.078 +/- 0.009 and 0.172 +/- 0.027, respectively, in the copper ion reducing antioxidant capacity (CUPRAC) assay. When the DNA cleavage activities of NO and the NOAuNPs were evaluated via hydrolysis, both samples cleaved DNA starting at a concentration of 25 g/mL in the cell culture analysis, while the nanoformulation of the NO components gave greater therapeutic and anticancer effects. We determined that the Au nanoparticles were not toxic to A549 cells. Moreover, after treatment with the half-maximal inhibitory concentration (IC50), determined by the MTT assay with A549 cells, we found that at 24 and 48 h, while the necrosis rates were high in cells treated with NO, the rates of apoptosis were greater in cells treated with NOAuNPs. Notably, for anticancer treatment, activating apoptotic pathways that do not cause inflammation is preferred. We believe that these results will pave the way for the use of NOAuNPs in in vitro studies of other types of cancer. Conclusion In this study, AuNPs were successfully synthesized from N. officinale extract. The biosynthesized AuNPs exhibited toxicity to and apoptotic effects on A549 lung cancer cells. Based on these findings, we suggest that green-synthesized AuNPs are promising new therapeutic agents for lung cancer treatment. However, since this was an in vitro study, further research should be performed in in vivo lung cancer models to support our findings and to explain the mechanism of action at the molecular level.
Chitosan Nanoparticles Loaded with Quercetin and Valproic Acid: A Novel Approach for Enhancing Antioxidant Activity against Oxidative Stress in the SH-SY5Y Human Neuroblastoma Cell Line
(Mdpi, 2024) Canbolat, Fadime; Demir, Neslihan; Yayintas, Ozlem Tonguc; Pehlivan, Melek; Eldem, Asli; Ayna, Tulay Kilicaslan; Senel, Mehmet
Background: Multiple drug-delivery systems obtained by loading nanoparticles (NPs) with different drugs that have different physicochemical properties present a promising strategy to achieve synergistic effects between drugs or overcome undesired effects. This study aims to develop a new NP by loading quercetin (Que) and valproic acid (VPA) into chitosan. In this context, our study investigated the antioxidant activities of chitosan NPs loaded with single and dual drugs containing Que against oxidative stress. Method: The synthesis of chitosan NPs loaded with a single (Que or VPA) and dual drug (Que and VPA), the characterization of the NPs, the conducting of in vitro antioxidant activity studies, and the analysis of the cytotoxicity and antioxidant activity of the NPs in human neuroblastoma SH-SY5Y cell lines were performed. Result: The NP applications that protected cell viability to the greatest extent against H2O2-induced cell damage were, in order, 96 mu g/mL of Que-loaded chitosan NP (77.30%, 48 h), 2 mu g/mL of VPA-loaded chitosan NP (70.06%, 24 h), 96 mu g/mL of blank chitosan NP (68.31%, 48 h), and 2 mu g/mL of Que- and VPA-loaded chitosan NP (66.03%, 24 h). Conclusion: Our study establishes a successful paradigm for developing drug-loaded NPs with a uniform and homogeneous distribution of drugs into NPs. Chitosan NPs loaded with both single and dual drugs possessing antioxidant activity were successfully developed. The capability of chitosan NPs developed at the nanometer scale to sustain cell viability in SH-SY5Y cell lines implies the potential of intranasal administration of chitosan NPs for future studies, offering protective effects in central nervous system diseases.
Chromogenic sensing, biological, and optical properties of Schiff bases of 2-amino-6-methoxybenzothiazole
(Elsevier B.V., 2024) Akhüseyin Yıldız, Elif; Pepe, Yasemin; Erdener, Diğdem; Karatay, Ahmet; Boyacıoğlu, Bahadır; Ünver, Hüseyin; Yapar, Gönül; Demir, Neslihan; Yıldız, Mustafa
In this study, a new series of Schiff base benzothiazole derivatives were synthesized and their structures were characterized by elemental analysis, FTIR, UV–VIS and NMR spectroscopy. The biological, sensory and photophysical properties of the benzothiazole derivatives were investigated. The sensing properties of the compounds were investigated UV–VIS spectroscopically, calorimetrically and theoretically. All compounds showed colorimetric, fluorometric and spectroscopic sensor properties for F−, CN−, AcO−, H2PO4− and OH− anions. Furthermore, DNA binding, DNA cleavage and antioxidant activities of compounds 1-4 were investigated. All compounds affected pBR322 DNA both hydrolytically and oxidatively, interacted with DNA in electrostatic mode, and also compounds 1 and 2 exhibited antioxidant activity close to standard BHT. In addition, the effects of electron donating and accepting substituents on the emission properties and charge transfer dynamics were investigated by fluorescence and femtosecond transient absorption spectroscopy measurements, respectively. According to fluorescence measurements, compounds 3 and 4 showed emission signal, while compounds 1 and 2 showed fluorescence quenching and weaker emission signal.
Determination of antioxidant activity and DNA damage protection of marchantia polymorpha L.
(Parlar Scientific Publications, 2021) Yayıntaş, Özlem Tonguç; Demir, Neslihan
In this study, the potential biological effects of Maarchantia polymorpha (Bryophyte) from Mount Ida (Crinakkale/Turkey) were investigated. M. polymorpha was extracted using the Soxhlet apparatus with several organic solvents (hexane, chloroform, acetone, ethanol, methanol, and aqueous) at different concentrations. Phytochemical screening tests are done to detect certain compounds. The total phenolic content was determined with the Folin-Ciocalteau method. Total flavonoid content was determined with AlCl3 method. Ethanolic extract was found to have higher total phenolic and flavonoid contents (18.24 GAE/g extract and 23.06 mgQE/g extract, respectively) than the other extracts. Antioxidant activities were examined by DPPH and ABTS radical scavenging assays. If polymorpha extracts showed that dose-dependent percent inhibition. The aqueous extract had the highest DPPH percentage of scavenging activity (70.87% at a concentration of 80 mu g/mL) followed by methanolic extract (68.59%). For ABTS radical scavenging activity, methanol extract showed the highest inhibition (73.12%) than other extracts. Various extracts were able to protect plasmid DNA by oxidative damage. The present study showed that polvmorpha could be a potential source of antioxidants and protective effects against plasmid DNA.
DFT calculation, biological activity, anion sensing studies and crystal structure of (E)-4-chloro-2-[(pyridin-2-ylimino)-methyl]phenol
(Serbian Chemical Soc, 2018) Yildirim, Nuray; Demir, Neslihan; Alpaslan, Gokhan; Boyacioglu, Bahadir; Yıldız, Mustafa; Unver, Huseyin
(E)-4-Chloro-2-[(pyridin-2-ylimino)methyl]phenol was synthesized in the reaction of 2-aminopyridine with 5-chlorosalicylaldehyde. The structure of compound was investigated by FTIR, UV-Vis, 1H-NMR, C-13-NMR and X-ray data. In addition, characterization of the compound was realized using theoretical quantum mechanical calculations and experimental spectroscopic methods. The molecular structure of the compound was confirmed using X-ray single-crystal data, NMR, FTIR and UV-Vis, which were in good agreement with the structure predicted by the theoretical calculations using the density functional theory (DFT). Moreover, the antimicrobial activity of the compound was investigated against some bacteria and yeast cultures by the broth microdilution test. UV-Vis spectroscopy studies of the interactions between the Schiff base and calf thymus DNA (CT-DNA) showed that the compound interacts with CT-DNA via electrostatic binding. The colorimetric response of the compound receptors was investigated before and after the addition of an equivalent amount of each anion to evaluate anion recognition properties.
DNA Interactions, Mutagenic, Anti-Mutagenic And Antimicrobial Activities of (E)-2-((3,5-Bis(Trifluoromethyl)Phenylimino)Methyl)-4,6- Dimethoxyphenol
(2021) Yıldırım, Nuray; Demir, Neslihan
Small molecules that interact with DNA are known to be effective as anticancer andantimicrobial agents. Therefore it is significant to search for new molecules interacting withDNA as potential new therapeutic agents. In this study, we aimed to investigate interactionsof novel fluorine substituted imine compound with DNA, (E)-2-((3,5-bis(trifluoromethyl)phenylimino)methyl)-4,6-dimethoxyphenol, and investigate its biological activities. DNAinteractions of the compound were investigated by UV-Vis absorption spectroscopy and gelelectrophoresis. The results demonstrated that the compound binds to DNA via intercalation.Agarose gel electrophoresis experiments showed that the compound does not cleave pBR322plasmid DNA hydrolytically or oxidatively. Furthermore, mutagenic, anti-mutagenic, andantimicrobial activities of the compound were studied by Ames and broth microdilution test,respectively. The compound showed mutagenic activity on both TA98 and TA100 strains. Also,the antimutagenic activity was observed in TA100 strain of S. typhimurium. It demonstratedantimicrobial activity against the microorganisms tested in the concentration range of 16-64µg/µL. The results show that the compound intercalates with DNA and has promising biologicalactivities.
Effect of group electronegativity on spectroscopic, biological, chromogenic sensing and optical properties of 2-formyl-benzene sulfonic acid sodium salt-based Schiff bases
(Elsevier B.V., 2023) Akhüseyin Yıldız, Elif; Pepe, Yasemin; Erdener, Diğdem; Karatay, Ahmet; Boyacıoğlu, Bahadır; Ünver, Hüseyin; Yapar, Gönül; Demir, Neslihan; Yıldız, Mustafa; Elmalı, Ayhan
In this study, 2-formylbenzenesulfonic acid sodium salt-based Schiff bases were synthesized from the reactions of 2-formylbenzenesulfonic acid sodium salt with 2-amino-4-chlorophenol and 2-amino-4-methylphenol. The structure of the compounds was determined by FTIR, UV–Vis and NMR spectroscopy methods using both experimental and DFT methods. The anion sensor properties of the compounds were investigated both UV–Vis spectroscopically and calorimetrically. In addition, the antimicrobial activities, interactions with DNA, DNA cleavage and antioxidant activities of Schiff bases were investigated. Additionally, the effects of group electronegativity on the spectroscopic, biological, chromogenic sensing and optical properties of chlorine and methyl side groups and sodium 2-sulfobenzalidene-aminophenol-based Schiff bases were investigated.
EFFECTS OF DIFFERENT DOSES OF SHIITAKE MUSHROOM (LENTINUS EDODES) ON SPERM PARAMETERS AND TESTICULAR TISSUE IN RATS: AN EXPERIMENTAL STUDY
(Pakistan Agricultural Scientists Forum, 2023) Büyük, Başak; Demir, Neslihan; Eroğlu, Hüseyin Avni; Öztopuz, Rahime Özlem
Lentinus edodes has been cultivated and used as food and medicine in far eastern countries.The present study aimed to reveal whether L.edodes that is used for different purposes has a toxic effect on the male genital system and to investigate its relationship with male infertility by showing its effects on sperm parameters. Twenty-four male Wistar rats were randomly divided into three groups as Control (C), Low-dose (LD) and High-dose (HD). Rats in Control were administered saline physiological once a day for 7 days. The LD and HD groups received 100 and 400mg/kg of L.edodes extract, respectively, once daily for 7 days. At the end of 7 days of administration of treatments, semen analysis, micronucleus analysis, gene expression levels and testicular Johnsen scoring was performed. Comparisons between groups without normal distribution were analyzed using Kruskal-Wallis variance analysis and those between groups with normal distribution were analyzed using one-way analysis of variance, followed by Tukey's test. P values of <0.05 were considered statistically significant. It is demonstrated that both low and high doses of extract significantly reduced spermatogenesis in seminiferous tubules found in the testicles of rats. In addition, high-dose administration resulted in decreased total sperm counts, whereas the progressive and non-progressive sperm motility counts were significantly decreased in both experimental groups. The gene expression levels of Casp-3 and TNF-alpha were increased in both the LD and HD groups compared with that in the C. Oral administration of low and high doses of L.edodes led to reduced spermatogenesis in the testicles, tissue damage to the testicle and decreased motile and total sperm counts.
Experimental, DFT Calculation, Biological Activity, Anion Sensing Application Studies and Crystal Structure of (E)-4-[(pyridin-3-ylimino)methyl]benzene-1,3-diol
(Springer/Plenum Publishers, 2018) Unver, Huseyin; Boyacioglu, Bahadir; Zeyrek, Celal Tugrul; Yolal, Devrim; Yıldız, Mustafa; Yildirim, Nuray; Demir, Neslihan
Crystallographic and spectroscopic studies of (E)-4-[(pyridin-3-ylimino)methyl]benzene-1,3-diol have been performed. Optimized molecular structure, conformational analysis, theoretical vibrational band assignments, nonlinear optical effects, UV-Vis, properties have also been investigated by using density functional theory with the functional B3LYP using the 6-311++G(d,p) basis set. The antimicrobial activities of the compound were investigated for its minimum inhibitory concentration. The colorimetric response of the Schiff base receptors was investigated before and after the addition of an equivalent amount of each anion to evaluate anion recognition properties. A new Schiff base was prepared and characterized. Experimental and theoretical studies on a new Schiff base have been reported. The tautomeric stability of the compound was investigated by experimental and theoretical (DFT). The nonlinear optical (NLO) effects of the compound was predicted using DFT. The antimicrobial activity and anion-sensor properties of the compound were examined. [GRAPHICS] .
Fabrication and Characterization of Saffron Stamen Aqueous Extract Controlled Release System as Potential Topical Treatment of Thermal Burn Wounds
(John Wiley and Sons Inc, 2021) Özdemir, Burak; Pişkin, Mehmet Burçin; Gök, Bahar; Demir, Neslihan; Budama-Kılınç, Yasemin
In our study, we aimed the preparation of Saffron stamen aqueous extract (SSAE) loaded chitosan nanoparticles (CNPs) to be used topically for thermal burns. First, biological activity tests such as DNA binding, DNA cleavage and antibacterial activity were performed to determine the effectiveness of SSAE to be encapsulated. Additionally, a genotoxicity test was performed for the reliability of SSAE. Ionic gelation method was used for encapsulation, and SSAE-loaded CNPs were obtained. Average particle size, zeta potential, polydispersity index (PdI), morphology, and releasing kinetics of the SSAE-loaded CNPs and blank CNPs were determined. DNA binding showed that SSAE interacted with DNA electrostatically. It was determined that all doses of SSAE used in hydrolytic and oxidative DNA cleavage experiments cleave DNA. As a result of the Ames / Salmonella test, it was found that 1,25 mg SSAE was not genotoxic. Then, this concentration was used in nanoparticle synthesis. As a result of DLS analysis, it was determined that SSAE-loaded CNPs have an average size of 57.61±1.234 nm, 0.175±0.008 PdI, and +14±0.755 mV zeta potential. Encapsulation efficiency and loading capacity of SSAE-loaded CNPs calculated as 71.76 % and 2,64 % respectively. As a result of releasing kinetics, it was determined that 79.54 % of SSAE was released after 168 hours. Antibacterial results revealed that SSAE loaded CNPs were more effective against bacteria than blank SSAE.
Fomes fomentarius (L.) Fr. extracts as sources of an antioxidant, antimicrobial and antibiofilm agents
(University of Nis Faculty of Science and Mathematics, 2021) İrez, Eylül İrem; Doğru, Nurcihan Hacioğlu; Demir, Neslihan
This paper evaluated antioxidant, antimicrobial and antibiofilm activities of ethanol, methanol, acetone and chloroform extracts of lignicolous fungal species Fomes fomentarius (L.) Fr. (Polypodiaceae). Antiradical activity was evaluated by using DPPH (2,2-diphenyl-1-picrylhydrazyl) assay. The antimicrobial screening was carried out via disc diffusion and microdilution methods in order to estimate minimum inhibitory (MIC) and minimum bactericidal concentration (MBC) of analyzed extracts against seven standard bacteria and one yeast: Escherichia coli NRRL B-3704, Pseudomonas aeruginosa ATCC 27853, Proteus vulgaris ATCC 13315, Acinetobacter baumanii ATCC 19606, Bacillus subtilis ATCC 6633, Staphylococcus aureus ATCC 25923, S. haemolyticus ATCC 43252 and Candida albicans ATCC 10231. In vitro antibiofilm activities were investigated based on crystal violet binding assay. All the extracts showed higher antioxidant activity compared to BHT (butylated hydroxytoluene) which was used as a standard. Ethanol, methanol and acetone extracts of the tested macrofungus also showed higher antimicrobial effect against P. aeruginosa ATCC 27853 in comparison to the antibiotic penicillin (P10). The lowest MIC was recorded by ethanol extract against S. haemolyticus ATCC 43252 (0.625 µg/mL). The highest antibiofilm activity was also noticed against biofilm formed by S. haemolyticus ATCC 43252. The results indicated that the F. fomentarius tested represent potential source of natural bioactive compounds with respect to antimicrobial, antibiofilm and antioxidant activities. © 2021 İrez et al.
Graphene quantum dots: Synthesis, characterization, cell viability, genotoxicity for biomedical applications
(Elsevier, 2019) Senel, Behiye; Demir, Neslihan; Buyukkoroglu, Gulay; Yıldız, Mustafa
We report the synthesis and applications of a novel N-doped graphene quantum dots (GQDs) using hydrothermal reaction between citric acid and p-aminophenol. The synthesized N-doped GQDs have been characterized physico-chemically and evaluated its antioxidant, antimicrobial, DNA binding and cleavage activities. siRNA loading studies were performed and their effects on cells were evaluated. Obtained results indicate that monodisperse solution of N-doped GQDs has been obtained with particles size ca. similar to 10.9 +/- 1.3 nm. UV-Vis spectroscopy studies of the interactions between the N-doped GQDs and calf thymus DNA (CT-DNA) showed that the compound interact with CT-DNA via both intercalative and electrostatic binding. The DNA cleavage study showed that the N-doped GQDs cleaved DNA without any external agents. The antioxidant activity of N-doped GQDS was very active when compared to BHT. As the concentration of the compound increased, the antioxidant activity also increased. Cell viability assay demonstrated that the Ndoped GQDs showed cell viability (70%) when the concentration reached 200 mu g/mL for A549 and also MDA-MB-231, 150 mu g/mL for NIH-3T3 cell lines at 24 h incubation. N-doped GQDs were coated with Eudragit RS 100 and EphA2-siRNA was loaded. As a result of the studies on these formulations, it was concluded that there may be significant effects on A549 cells. The microscopy results revealed that N-doped GQDs was quickly internalized into the cell. Our novel N-doped-GQDs with siRNA are candidate for in situ tumor suppression via DNA and mRNA breakage. (C) 2019 The Authors. Production and hosting by Elsevier B.V. on behalf of King Saud University.
Hematological, serum biochemical, and immunological responses in common carp (Cyprinus carpio) exposed to phosalone
(Springer-Verlag London Ltd, 2015) Kaya, Hasan; Çelik, Ekrem Şanver; Yılmaz, Sevdan; Tulgar, Arınç; Akbulut, Mehmet; Demir, Neslihan
This study was conducted to investigate the effects of sublethal phosalone concentrations on the hematological, serum biochemical, and immunological responses of carp (Cyprinus carpio). The fish were semi-statically exposed to low (0.15 mg/L), medium (0.3 mg/L), and high (0.6 mg/L) phosalone concentrations for 14 days. Results showed that the hematological variables red blood cell count (RBC, ×106/mm3), white blood cell count (×103/mm3), hematocrit (Hct, %), hemoglobin (Hb) concentration (g/dL), corpuscular volume mean (MCV, ?m3), corpuscular Hb mean (MCH, pg), and mean corpuscular Hb concentration (MCHC, %) were significantly affected by phosalone concentrations. In addition, white blood cell types (neutrophil, lymphocyte, and monocyte) were also affected. Furthermore, serum proteins, metabolites, lipids, enzymes, and electrolytes were significantly altered by different phosalone concentrations. Nitroblue tetrazolium (NBT), lysozyme, and myeloperoxidase activities significantly altered treatment with phosalone concentrations. As a result, phosalone resulted in significant physiological and behavioral changes in C. carpio. These changes may potentially impair C. carpio’s survival capability in natural habitats or aquaculture. © 2014, Springer-Verlag London.
Investigation of Antimicrobial, Antibiofilm and Antioxidant Activities Dichodontium pellucidum (Hedw.) Schimp
(Mevlüt AKÇURA, 2023) Uyar, Güray; Demir, Neslihan; Hacıoğlu, Nurcihan
Dichodontium pellucidum (Hedw.) Schimp., Dicranaceae familyası üyesi; Avrupa, Asya, Kuzey Amerika ve Afrika gibi çeşitli bölgelerde yayılış gösteren bir karayosunudur. Bu çalışmada, D. pellucidum karayosununun dört farklı ekstraktının (etanol, metanol, aseton ve etil asetat) antimikrobiyal, antibiyofilm ve antioksidan potansiyelleri araştırılmıştır. Antimikrobiyal aktiviteyi değerlendirmek için disk difüzyon, minimum inhibitör konsantrasyon, ve minimum bakterisidal konsantrasyon testleri yapılmıştır. Karayosunu ekstraktlarının biyofilm oluşumu (antibiyofilm) üzerine etkileri, mikroplaka biyofilm metoduna göre değerlendirilmiştir. Ekstraktların antioksidan aktiviteleri serbest radikal temizleme yöntemi olarak 2,2-diphenyl-1-picrylhydrazyl (DPPH) ve CUPRAC kullanılarak ölçülmüştür. Karayosunu ekstraktları bakteriler için 8.0-16.0 mm, maya kültürü için 8.0-13.0 mm arasında değişen inhibisyon zonlarıyla tüm test mikroorganizmalarına karşı antimikrobiyal aktivite göstermiştir. En yüksek antimikrobiyal ve antibiyofilm aktivite Bacillus subtilis ATCC 6633 bakterisine karşı etil asetat ekstraktında saptanmıştır. Metanol çözgeninin diğer çözücülerden daha etkili antioksidan aktivite sergilediği gözlenmiştir. Elde edilen bulgular, D. pellucidum yüksek potansiyelinden dolayı doğal antimikrobiyal ve antioksidan ajan araştırmalarında ve biyofilm kontrolünde tercih edilebileceğini kanıtlamıştır. Bu çalışma, D. pellucidum biyoaktivitesi için ilk rapordur. Bu yaklaşım aynı zamanda medikal kullanım veya ilaç araştırma geliştirmeleri için yeni araştırmalara izin verebilir.

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