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Öğe Alpha-synuclein levels in multiple sclerosis patients with restless leg syndrome(Cukurova Univ, Fac Medicine, 2020) Cakina, Suat; Yucel, Selma; Polat, Cemre Cagan; Ozturk, SamilPurpose: The restless legs syndrome is more frequent and critical in Multiple Sclerosis patients, and it harms their general well-being and life quality. Alpha-synuclein is a synuclein protein that can have an impact on the pathway of signaling, affecting the Dopamin 2 receptor and its receptor trafficking. Studies have shown that the decrease in dopamine 2 receptor and Restless Legs Syndrome disease are correlated. This study is aimed to ascertain the alpha-synuclein level in multiple sclerosis patients with restless legs syndrome. Materials and Methods: We took blood samples from 40 multiple sclerosis patients and 20 healthy individuals. Half of the patient group had Multiple Sclerosis with restless leg syndrome. In the study groups, the alpha-synuclein level was determined by quantitative real-time polymerase chain reaction (qRT-PCR) and enzyme-dependent immunosorbent assay (ELISA). Results: Alpha-synuclein gene expression level was found or be significantly lower in restless leg syndrome patients with multiple sclerosis than the Alpha-synuclein gene expression level in the control group. Conclusion: Alpha-synuclein may have an impact on the pathogenesis of the restless leg syndrome of multiple sclerosis disease. Further investigations are required to determine the impact of alpha-synuclein in the pathogenesis of restless leg syndrome in multiple sclerosis disease.Öğe Does Gender Difference Effect Radiation-Induced Lung Toxicity? An Experimental Study by Genetic and Histopathological Predictors(Radiation Research Soc, 2022) Cosar, Rusen; Ozen, Alaattin; Tastekin, Ebru; Sut, Necdet; Cakina, Suat; Demir, Selma; Parlar, SuleSeveral studies have reported differences in radiation toxicity between the sexes, but these differences have not been tested with respect to histopathology and genes. This animal study aimed to show an association between histopathological findings of radiation-induced lung toxicity and the genes ATM, SOD2, TGF-beta 1, XRCC1, XRCC3 and HHR2. In all, 120 animals were randomly divided into 2 control groups (male and female) and experimental groups comprising fifteen rats stratified by sex, radiotherapy (0 Gy vs. 10 Gy), and time to sacrifice (6, 12, and 24 weeks postirradiation). Histopathological evaluations for lung injury, namely, intra-alveolar edema, alveolar neutrophils, intra-alveolar erythrocytes, activated macrophages, intra-alveolar fibrosis, hyaline arteriosclerosis, and collapse were performed under a light microscope using a grid system; the evaluations were semi quantitatively scored. Then, the alveolar wall thickness was measured. Real-time quantitative reverse transcription PCR (RT-qPCR) was used to determine gene expression differences in ATM, TGF-beta 1, XRCC1, XRCC3, SOD2 and HHR2L among the groups. Histopathological data showed that radiation-induced acute, subacute, and chronic lung toxicity were worse in male rats. The expression levels of the evaluated genes were significantly higher in females than males in the control group, but this difference was lost over time after radiotherapy. Less toxicity in females may be attributable to the fact that the expression of the evaluated genes was higher in normal lung tissue in females than in males and the changes in gene expression patterns in the postradiotherapy period played a protective role in females. Additional data related to pulmonary function, lung weights, imaging, or outcomes are needed to support this data that is based on histopathology alone. (C) 2022 by Radiation Research SocietyÖğe Evaluation of thiol/disulfide homeostasis in patients with gestational diabetes mellitus(Taylor & Francis Ltd, 2020) Cakina, Suat; Aydin, Buket; Beyazit, FatmaThis study aimed to study the dynamic thiol/disulfide homeostasis in pregnant women with gestational diabetes mellitus (GDM). Forty-five pregnant women with GDM and 45 age-matched healthy pregnancies were included in this study. Thiol/disulfide homeostasis was measured using a commercial kit (Rel Assay Diagnostics). The patients with GDM had significantly higher disulfide concentrations than healthy pregnant patients (p = .001). Besides, the GDM group had significantly higher disulfide/total thiol, disuplhide/native thiol, and native thiol/total thiol ratio than healthy pregnant patients (p = .001, p = .001 and p = .001, respectively). The significantly higher concentrations of disulfide, disulfide/total thiol, disuplhide/native thiol, and native thiol/total thiol ratio in women with GDM could be considered as the increased oxidative stress.Öğe Oxidative stress in preeclampsia: A comparative biomarker analysis across body mass index categories(Sciendo, 2025) Cakina, Suat; Postaci, Emine Sevinc; Beyazit, FatmaBackground Preeclampsia is a complex form of prenatal hypertension associated with endothelial dysfunction, systemic inflammation, and oxidative stress. This study aimed to determine the amounts of malondialdehyde, total oxidant status, and oxidative stress index in the blood of pregnant women with preeclampsia and controls, as well as how these parameters varied with body mass index. Methods The present study was a case-control study carried out in the Department of Obstetrics and Gynecology, Faculty of Medicine & Ccedil;anakkale Onsekiz Mart University, Turkiye, from July 2023 to May 2025. The study included 50 pregnant women with preeclampsia and 50 with normotension (control group). They were divided into three groups: normal weight, overweight, and obese, using body mass index. Serum malondialdehyde, total oxidant capacity, total antioxidant capacity, and oxidative stress index levels were measured using validated spectrophotometric techniques. Statistical analyses were performed using SPSS version 25.0, with a significance level of p<0.05. Results Malondialdehyde, total oxidant capacity, and oxidative stress index levels were significantly higher in the preeclampsia group than in the control group. Compared with the control group, total antioxidant capacity levels were significantly lower, particularly in the obese preeclampsia subgroup (p<0.05). Conclusions According to this study, preeclamptic women's oxidative stress rose as their body mass index rose. Our study's observed values suggest that they may be applied as prognostic or diagnostic biomarkers for preeclampsia. Our research might help create tailored risk evaluations and focused antioxidant treatments for high-risk pregnancies.Öğe Oxidative stress markers in liver in streptozocin-induced diabetic rats: effects of metformin and sitagliptin(Cukurova Univ, Fac Medicine, 2020) Cakina, Suat; Ozturk, SamilPurpose: This study aims at investigating the oxidative stress effect of antidiabetic drugs of Metformin (MET) and sitagliptin (STG) in the liver tissue of diabetic rats from streptozotocin (STZ). Materials and Methods: Thirty-five female Wistar rats (3-4 months old, weighing 200 +/- 25 g) were divided into five groups (with seven rats each) and treated as follows: control (Cont), streptozotocin alone (STZ), streptozotocin + metformin (STZ+MET), streptozotocin + sitagliptin (STZ+STG), streptozotocin + metformin + sitagliptin (STZ+MET+STG). Sitagliptin, Metformin, and combined metformin sitagliptin treated subgroups for four weeks. Malondialdehyde (MDA), total antioxidant status (TAS), total oxidant status (TOS) levels, and oxidative stress index (OSI) ratio were measured in rat liver tissue. Besides, cells undergoing apoptotic cell death were determined using the TUNEL technique through histopathological evaluation. Results: MDA, TAS, and OSI of STZ+MET+STG administered group decreased compared to STZ. TOS of STZ+MET+STG administered group decreased compared to STZ. Conclusion: In the experimental T2D model in rats, it shows protective effect when sitagliptin is used with metformin against oxidative damage in liver tissue caused by STZ.Öğe OXIDATIVE STRESS PARAMETERS IN PATIENTS WITH MIGRAINE WITHOUT(2020) Cakina, Suat; Yücel, Selma; Polat, Cemre Cagan; Oztürk, ŞamilAim: Migraine is a multifaceted neurological disease whose molecular mechanisms are not yet clearly defined. Oxidative stress is also believed to play a role in the pathogenesis of migraine. The purpose of this study was to evaluate serum paraoxonase (PON) and arylesterase (ARE) activities, thiols levels, ischemia-modified albumin (IMA), the total oxidant status (TOS), total antioxidant status (TAS), and oxidative stress index (OSI) in patients with migraine without aura. Materials and Methods: 30 patients (5 males and 25 females) and 30 matched healthy controls (8 males and 22 females) were enrolled. Serum PON and ARE activities, thiols levels, IMA, TAS, TOS and OSI were determined by using the spectrophotometric method in the study groups. Statistical analysis was conducted using Mann–Whitney U test and independent samples t-test, and p<0.05 was considered as statistically significant. Results: PON and ARE levels were significantly lower in the migraine without aura group compared with the control group p<0.05. Conclusion: Our results suggested that the PON and ARE levels might be associated with migraine without aura. In migraine patients, it is recommended that oxidative stress markers be investigated with a larger population and these parameters are taken into consideration.Öğe Relationship between genetic polymorphisms MTHFR (C677T, A1298C), MTR (A2756G) and MTRR (A66G) genes and multiple sclerosis: a case-control study(Termedia Publishing House Ltd, 2019) Cakina, Suat; Ocak, Ozgul; Ozkan, Adile; Yucel, Selma; Karaman, Handan Isin OzisikRecent studies have reported elevated plasma homocysteine and reduced folate and vitamin B levels in patients with multiple sclerosis (MS). In this study, we aimed to investigate the association between MS and the following four DNA polymorphisms: MTR A[2756]G, MTHFR C[677]T, MTHFR A[1298]C and MTRR A[66]G. The DNA polymorphisms were genotyped in 80 patients with confirmed MS and 80 healthy control age- and gender-matched subjects using PCR-RFLP approach. Our results show that the frequency of the T/T genotype homozygotes for the MTHFR C[677]T polymorphism was significantly higher in patients than in controls (p = 0.04, OR: 3.16, 95% CI: 1.23-8.17). In turn, the A/A genotype of the MTHFR A[1298]C polymorphism was more frequent in controls than in patients (41.3% vs. 32.5%, p = 0.04). There were no differences in distribution of genotypes for the MTR A[66]G and MTR A[2756]C polymorphisms between patients with MS and controls (p > 0.05). Our findings suggested that the MTHFR C[677]T and MTHFR A[1298] C gene polymorphisms might be associated with MS as genetic factors influencing the risk of the disease.Öğe The role of paraoxonase and myeloperoxidase as oxidative stress markers in pregnant women with hypothyroidism(Taylor & Francis Ltd, 2022) Cakina, Suat; Pek, Eren; Ozkavak, Onur; Kocyigit, Deniz; Beyazit, FatmaObjective: This study aims to measure paraoxonase 1 (PON 1) and myeloperoxidase (MPO) levels in patients diagnosed with hypothyroidism during pregnancy by spectrophotometric method and to compare the results with healthy controls. Methods: For the study, the blood samples of 45 hypothyroid pregnant patients and 45 healthy control pregnant individuals who were compatible with the patient group in terms of age were taken. PON 1 and MPO levels in both groups were measured using spectrophotometric methods and statistical analysis was performed. Results: Serum PON 1 activity levels were significantly lower in hypothyroid pregnant patients than in the control group (p=.020). The patients group had significantly higher serum MPO concentrations than in control group (p=.020). MPO/PON-1 ratio was higher in pregnant patients with hypothyroidism than in the control group (p=.030). Conclusion: Decreased levels of PON1 with increased levels of MPO in hypothyroid pregnant patients suggest the critical role of oxidative stress in hypothyroidism during pregnancy.Öğe Vitamin D receptor gene polymorphisms in multiple sclerosis disease: A case-control study(Univ Press, 2018) Cakina, Suat; Ocak, Ozgul; Ozkan, Adile; Yucel, Selma; Karaman, Handan Isin OzisikMultiple sclerosis (MS) is a common neurologic disorder that is a chronic inflammatory, demyelinating, and neurodegenerative disease of the central nervous system (CNS). Its etiology remains unknown. Several recent studies have found that decreased susceptibility to vitamin D deficiency is also associated with a decreased risk of MS. The role of vitamin D receptor (VDR) gene and its polymorphisms are highlighted as susceptible components. In this study, we aimed to identify the relationship between Apal (rs7975232), BsmI (rs 1544410), and TaqI (rs731236) gene polymorphisms with MS. Apal, Bsml, and TaqI genotypes were determined in 70 patients with MS and in 70 control subjects. DNA was isolated from blood samples, and then Apal, BsmI and TaqI gene polymorphisms were identified using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay. The distribution of BsmI and TaqI polymorphisms did not show any significant differences in MS patients and controls; however, increased A allele of Apal polymorphism was found in MS patients. Our findings suggest that the Apal gene polymorphism might be associated with MS. Investigation of a larger population and functional work on these gene structures and function in MS patients are recommended.
