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Öğe 3D HYDROGELS FROM DETERGENT-FREE DECELLULARIZED SPINAL CORD MENINGES REINFORCED WITH HYDROPHILIC SILK FIBROIN FOR REGENERATIVE MEDICINE APPLICATIONS(Mary Ann Liebert, Inc, 2024) Kurt, Tuğçe; Arslan, Yavuz Emre[No abstract available]Öğe 3D Printing of Extracellular Matrix-Based Multicomponent, All-Natural, Highly Elastic, and Functional Materials toward Vascular Tissue Engineering(John Wiley and Sons Inc, 2023) Işık, Melis; Karakaya, Ece; Arslan, Tugba Sezgin; Atila, Deniz; Erdoğan, Yaşar Kemal; Arslan, Yavuz Emre3D printing offers an exciting opportunity to fabricate biological constructs with specific geometries, clinically relevant sizes, and functions for biomedical applications. However, successful application of 3D printing is limited by the narrow range of printable and bio-instructive materials. Multicomponent hydrogel bioinks present unique opportunities to create bio-instructive materials able to display high structural fidelity and fulfill the mechanical and functional requirements for in situ tissue engineering. Herein, 3D printable and perfusable multicomponent hydrogel constructs with high elasticity, self-recovery properties, excellent hydrodynamic performance, and improved bioactivity are reported. The materials' design strategy integrates fast gelation kinetics of sodium alginate (Alg), in situ crosslinking of tyramine-modified hyaluronic acid (HAT), and temperature-dependent self-assembly and biological functions of decellularized aorta (dAECM). Using extrusion-based printing approach, the capability to print the multicomponent hydrogel bioinks with high precision into a well-defined vascular constructs able to withstand flow and repetitive cyclic compressive loading, is demonstrated. Both in vitro and pre-clinical models are used to show the pro-angiogenic and anti-inflammatory properties of the multicomponent vascular constructs. This study presents a strategy to create new bioink whose functional properties are greater than the sum of their components and with potential applications in vascular tissue engineering and regenerative medicineÖğe 3D-Printable, Self-Stiffening (4D) and Shape Morphing Hydrogel through Single-Step Orthogonal Crosslinking of Phenolic Biopolymers for Dynamic Tissue Engineering(Wiley, 2025) Gungor, Nuriye Nazet; Kurt, Tugce; Sari, Buse; Isik, Melis; Okesola, Babatunde O.; Arslan, Yavuz Emre; Derkus, BurakParticularly for dynamic, shape-changing, or fibrillar tissues such as muscles and blood vessels, the development of innovative biomaterials is crucial for advancing tissue engineering and regenerative medicine. This study introduces a novel multicomponent hydrogel created from silk fibroin (SF), tyramine-modified hyaluronic acid (HA_Tyr), and tyramine-modified gelatin (G_Tyr). Using an enzymatic orthogonal covalent bonding between phenolic groups, i.e., tyrosine and tyramine moieties of SF, HA_Tyr, and G_Tyr, a dynamically stiffening SF/HA_Tyr/G_Tyr (SHG) multicomponent hydrogel is achieved with enhanced mechanical properties. Utilizing an extrusion-based 3D printing approach, the precise fabrication of constructs with tailored geometries and functionalities is demonstrated. The emerging 3D-printed hydrogels undergo morphologic changes (4D) under 37 degrees C/phosphate buffer saline (PBS) conditions. The observed morphological change results from the conformational change and folding of SF leading to fibrillation. These multicomponent hydrogels also show significant promise in creating bio-instructive materials that meet the mechanical and functional requirements necessary for in situ tissue engineering. The study highlights the potential of these self-stiffening biomaterials to recover dynamic and fibrillar tissues, supported by both in vitro and pre-clinical chorioallantoic membrane (CAM) model evaluations that underscore their biocompatibility and pro-angiogenic properties.Öğe A Facile Strategy for Preparing Flexible and Porous Hydrogel-Based Scaffolds from Silk Sericin/Wool Keratin by In Situ Bubble-Forming for Muscle Tissue Engineering Applications(Wiley-V C H Verlag Gmbh, 2024) Demiray, Elif Beyza; Sezgin Arslan, Tuğba; Derkuş, Burak; Arslan, Yavuz EmreIn the present study, it is aimed to fabricate a novel silk sericin (SS)/wool keratin (WK) hydrogel-based scaffolds using an in situ bubble-forming strategy containing an N-(3-dimethylaminopropyl)-N '-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) coupling reaction. During the rapid gelation process, CO2 bubbles are released by activating the carboxyl groups in sericin with EDC and NHS, entrapped within the gel, creating a porous cross-linked structure. With this approach, five different hydrogels (S2K1, S4K2, S2K4, S6K3, and S3K6) are constructed to investigate the impact of varying sericin and keratin ratios. Analyses reveal that more sericin in the proteinaceous mixture reinforced the hydrogel network. Additionally, the hydrogels' pore size distribution, swelling ratio, wettability, and in vitro biodegradation rate, which are crucial for the applications of biomaterials, are evaluated. Moreover, biocompatibility and proangiogenic properties are analyzed using an in-ovo chorioallantoic membrane assay. The findings suggest that the S4K2 hydrogel exhibited the most promising characteristics, featuring an adequately flexible and highly porous structure. The results obtained by in vitro assessments demonstrate the potential of S4K2 hydrogel in muscle tissue engineering. However, further work is necessary to improve hydrogels with an aligned structure to meet the features that can fully replace muscle tissue for volumetric muscle loss regeneration. A novel hydrogel-based bioengineered scaffold with a porous and flexible ultrastructure is fabricated via in situ crosslinking of sericin and keratin. In chorioallantoic membrane analysis, the bioengineered scaffold not only shows angiogenic potential but also promotes the biological behavior of C2C12 muscle cells. These results highlight the potential of the sericin/keratin scaffold for future applications in repairing volumetric muscle tissue loss. imageÖğe A novel method for constructing an acellular 3D biomatrix from bovine spinal cord for neural tissue engineering applications(Wiley, 2019) Arslan, Yavuz Emre; Efe, Burcu; Arslan, Tugba SezginIn this study, we aimed at generating 3-dimensional (3D) decellularized bovine spinal cord extracellular matrix-based scaffolds (3D-dCBS) for neural tissue engineering applications. Within this scope, bovine spinal cord tissue pieces were homogenized in 0.1 M NaOH and this viscous mixture was molded to attain 3D bioscaffolds. After resultant bioscaffolds were chemically crosslinked, the decellularization process was conducted with detergent, buffer, and enzyme solutions. Nuclear remnants in the native tissue and 3D-dCBS were determined with DNA content analysis and agarose gel electrophoresis. Afterward, 3D-dCBS were biochemically characterized in depth via glycosaminoglycan (GAG) content, hydroxyproline (HYP) assay, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Cellular survival of human adipose-derived mesenchymal stem cells (hAMSCs) on the 3D-dCBS for 3rd, 7th, and 10th days was assessed via MTT assay. Scaffold and cell/scaffold constructs were also evaluated with scanning electron microscopy and histochemical studies. DNA contents for native and 3D-dCBS were respectively found to be 520.76 +/- 18.11 and 28.80 +/- 0.20 ng/mg dry weight (n = 3), indicating a successful decellularization process. GAG content, HYP assay, and SDS-PAGE results proved that the extracellular matrix was substantially preserved during the decellularization process. In conclusion, it is believed that the novel decellularization method may allow fabricating 3D bioscaffolds with desired geometry from soft nervous system tissues.Öğe A preliminary study on the development of a novel biomatrix by decellularization of bovine spinal meninges for tissue engineering applications(Springer, 2021) Özüdoğru, Eren; Arslan, Yavuz EmreHere, we aim at developing a novel biomatrix from decellularized bovine spinal meninges for tissue engineering and regenerative medicine applications. Within this concept, the bovine spinal meninges were decellularized using 1% Triton X-100 for 48 h, and residual nuclear content was determined with double-strand DNA content analysis and agarose gel electrophoresis. The major matrix components such as sulfated GAGs and collagen before and after the decellularization process were analyzed with DMMB, hydroxyproline assay and SDS-PAGE. Subsequently, the native bovine spinal meninges (nBSM) and decellularized BSM (dBSM) were physiochemically characterized via ATR-FTIR spectroscopy, TGA, DMA and tensile strength test. The dsDNA content in the nBSM was 153.39 +/- 53.93 ng/mg dry weight, versus in the dBSM was 39.47 +/- 4.93 ng/mg (n = 3) dry weight and DNA fragments of more than 200 bp in length were not detected in the dBSM by agarose gel electrophoresis. The sulfated GAGs contents for nBSM and dBSM were observed to be 10.87 +/- 1.2 and 11.42 +/- 2.01 mu g/mg dry weight, respectively. The maximum strength of dBSM in dry and wet conditions was found to be 19.67 +/- 0.21 MPa and 13.97 +/- 0.17 MPa, while nBSM (dry) was found to be 26.26 +/- 0.28 MPa. MTT, SEM, and histology results exhibited that the cells attached to the surface of dBSM, and proliferated on the dBSM. In conclusion, the in vitro preliminary study has demonstrated that the dBSM might be a proper and new bioscaffold for tissue engineering and regenerative medicine applications.Öğe Assessment of the Cytotoxicity of Melia azedarach L. Extracts on Human Adipose-derived Mesenchymal Stem Cells(2018) Efe, Burcu; Galata, Yusuf Furkan; Arslan, Yavuz EmreIn this study, aqueous extracts of Melia azedarach L. green fruit and leaves were obtained using two different extraction methods. The extraction yields of the green fruit and leaves were found as 24.11% and 37.98% for the infusion method; 17.76% and 27.00% for the rotating method, respectively. The total phenolic content, related to the infusion method, was ascertained for green fruit extract 173.67±10.84 mg Gallic Acid Equivalent (GAE)/g dry weight and leaf extract 312.33±9.81 mg GAE/g dry weight. In other respects, antioxidant activity related to the infusion method was determined for green fruit extract 172.51±13.23 mg Trolox/L and leaf extract 569.16±10.41 mg Trolox/L. Gas chromatography-mass spectrometry (GC-MS) analysis was performed to identify the chemical composition of the extracts. The cytotoxicity levels of the extracts were assessed on human adipose-derived mesenchymal stem cells (hAMSCs) using commercially available XTT assay. Consequently, it has been found that the green fruit extract has more cytotoxic activity than the leaf extract on hAMSCs.Öğe Avant-Garde Hydrogels as Stem Cell Niche for Cardiovascular Regenerative Medicine(Springer Nature, 2023) Yilmaz, Hilal Deniz; Arslan, Yavuz EmreCardiovascular diseases remain the primary cause of death in the modern world. According to the World Health Organization (WHO), almost 18 million people lose their lives each year due to cardiac dysfunction. Nearly 75% of the cases are related to heart attack, stroke, and heart failure. Furthermore, the limited restoration capacity of the adult cardiac tissue leads to irreversible changes in myocardial injury and ischemia, which seriously increases the mortality rates. Currently, the available therapeutic approaches for cardiovascular dysfunctions mainly depend on pharmaceutical drugs, vascular assist devices, or organ transplantations in severe cases. However, these strategies cannot prevent ischemia-related damages or restore the dysfunction of heart tissue. Besides, there are certain limitations, including donor shortage, thrombosis of the device, immune rejection reactions, and operative morbidity of the patient. Therefore, combining stem cells with outstanding hydrogels has become a critically important phenomenon in treating cardiovascular disease. Today, stem cell-based applications form the pivot point of regenerative treatments due to their differentiation and immunomodulatory capacity. However, due to low cellular retention and the inhomogeneous therapeutic activities, the effectiveness of stem cell regeneration in clinical and preclinical studies is reduced. Hence, the synergetic approaches of the injectable hydrogels with stem cells and subcellular bioactive tools may hold the promises of the next-generation therapies. Over the last decade, various functional hydrogels from natural, synthetic, or decellularized tissue precursors have been developed and investigated for cardiovascular applications. In this chapter, advances in stem cell therapy have been discussed with the latest research on functional hydrogels for cardiovascular regeneration. © The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Nature Singapore Pte Ltd. 2023.Öğe Beyond traditional dentistry: How organoids and next-gen hydrogels are redesigning dental tissue regeneration(Elsevier, 2026) Yilmaz-Dagdeviren, Hilal Deniz; Arslan, Yavuz EmreDental tissue regeneration has advanced rapidly with the development of bioengineered hydrogels and organoid technologies. In this review, multifunctional hydrogels are examined as biomimetic platforms with osteoinductive, adhesive, angiogenic, antimicrobial, and immunomodulatory properties tailored to enamel, dentin-pulp complex, periodontal ligament, and alveolar bone repair. Incorporation of bioactive molecules, including growth factors, bioceramics, antioxidants, and immune-modulating agents, has been reported to enhance tissue-specific regeneration while mitigating infection and inflammation. Stimuli-responsive designs have been utilized to enable spatiotemporally controlled delivery and degradation. Immunomodulatory hydrogels also have been shown to direct macrophage polarization, regulate T-cell infiltration, and promote matrix remodeling. Furthermore, organoid models supported by hydrogels have been employed to replicate dental tissue architecture, guide lineage-specific differentiation, and provide reproducible, physiologically relevant platforms for drug screening and developmental studies. Emerging strategies such as microfluidic organoid-on-chip systems and mechanically stimulated cultures are noted for their potential to provide more physiologically relevant models. Early clinical studies involving hydrogel-based scaffolds and stem cell constructs are discussed, indicating growing translational potential. Overall, these developments highlights that how advanced hydrogels and organoid systems can contribute to a shift from conventional restorative methods toward tissue engineering-based regenerative therapies.Öğe Bioengineered three-dimensional physical constructs from quince seed mucilage for human adipose-derived mesenchymal stem cells(Sage Publications Ltd, 2020) Şimşek, Ekin; Karaca, Burak; Arslan, Yavuz EmreIn this study, we aimed at fabricating a novel porous physical construct from quince seed mucilage for translational medicine applications. To achieve this goal, quince seed mucilage was extracted, molded, and freeze-dried. After being freeze-dried, the molded constructs were chemically crosslinked with 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide/N-hydroxysuccinimide to maintain the mechanical integrity of the structure. The fabricated scaffolds were characterized in-depth by scanning electron microscopy, Fourier transform infrared spectroscopy, Brunauer-Emmett-Teller analysis, thermogravimetric analysis, and dynamic mechanical analysis in addition to the swelling, liquid uptake, and porosity tests. The extraction yield of mucilage was calculated to be 6.28% +/- 0.40% (n = 3). The swelling ratio of crosslinked quince seed mucilage-derived scaffolds was found to be 12,677.50% +/- 388.82% (n = 3), whereas the porosity of crosslinked quince seed mucilage-derived scaffolds was 83.43% +/- 2.84% (n = 3). The analyses confirmed the crosslinked quince seed mucilage-derived scaffolds to be possessed interconnected, highly porous structure. Afterward, human adipose-derived mesenchymal stem cells were seeded on the crosslinked quince seed mucilage-derived scaffolds, and the cell viability on the scaffolds was assessed with 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay. The MTT results revealed the scaffolds not to be possessed any cytotoxic effect on seeded cells. Human adipose-derived mesenchymal stem cells adhesion and migration on the crosslinked quince seed mucilage-derived scaffolds were also evaluated histologically using hematoxylin and eosin staining in addition to scanning electron microscopy analysis. In conclusion, we believe that crosslinked quince seed mucilage-derived scaffolds have the potential to be an alternative to routinely used polysaccharides in regenerative medicine applications.Öğe Clinical tissue engineering approach and biotechnological advances to improve athlete healthcare(Turkish Sports Medicine Association, 2023) Demiray, Elif Beyza; Kurt, Tuğçe; Duman, Zeynep Yağmur; Özdemir, Büşra Nur; Erkovan, Burak; Yiğit, Gaye Su; Arslan, Yavuz EmreSports activities have continued for centuries and have become essential to daily life. Professional athletes participating in various sports competitions have many advantages, such as a promising career and high income. On the other hand, being a professional athlete also has some disadvantages. The most dramatic one is the risk of injury. Even though injuries are a part of sports, they have become a significant problem today due to the long recovery period which in turn overshadows sports competitions. In addition, the performance loss is an extra handicap for the athletes compared to the pre-injury levels. In this case, biomedical and biotechnological sciences are a glimmer of hope for shortening the treatment process and minimizing performance loss in returning to professional sports life. Combinations of scaffolds, biological factors, and cells are utilized based on mentioned approaches to treat such injuries, which are frequently seen nowadays and have become the nightmare of professional athletes. This review discusses various regenerative medicine and biotechnology-based therapeutic methods used in the treatment of spinal cord, cartilage, tendon, and musculoskeletal injuries in athletes. Additionally, wearable technologies, which are used to evaluate physiological signals, monitor health, prevent possible injuries, and create personalized training programs are mentioned, as well.Öğe COVID-19 Tedavisinde Mezenkimal Kök Hücrelerin Potansiyel Kullanımı Üzerine Kapsamlı Bir İnceleme(Türkiye Sağlık Enstitüleri Başkanlığı, 2021) Kurt, Tugce; Sevinc, Isa; Uysal, Feyza; Demiray, Elif; Yılmaz, Hilal; Arslan, Yavuz Emreİlk olarak Çin’in Wuhan kentinde tespit edildiği düşünülen yeni tip koronavirüs (SARS-CoV-2), raporlandığı ilk vakadan bu yana kısa süre içinde tüm dünyayı etkisi altına alarak bir salgına dönüşmüştür. Virüs, COVID-19 adı verilen bulaşıcı bir hastalığa neden olarak 150 milyondan fazla kişiyi etkilemiştir. Ayrıca yoğun virüs yükü ile enfekte olmuş bireylerde oluşan sitokin fırtınasının hastalarda akut solunum yolu bozukluğu (ARDS), pulmoner fibrozis ve hatta çoklu organ yetmezliği gibi durumlara neden olabildiği görülmüştür. Yapılan in vitro ve preklinik çalışmalarda mezenkimal kök hücrelerin (MKH) rejeneratif özelliklerinin yanında anti-enflamatuar ve immünmodülatör etkilerinin olduğu belirlenmiştir. Bu nedenle bilim insanları, rejeneratif bir umut olarak COVID-19 tedavisinde konvansiyonel ilaç veya plazma temelli tedavilere alternatif olarak MKH’leri kullanmayı önermektedir. Böylece hastalarda yoğun ilaç kullanımına bağlı yan etkilerin görülmeden MKH terapisi ile immünmodülasyon ve anti-enflamatuar etkilerin sağlanabileceği ve ARDS, pulmoner fibrosiz, sepsis ve çoklu organ yetmezliği gibi olumsuz senaryoların önüne geçileceği düşünülmektedir. Ayrıca MKH'lerin rejenerasyon özelliği sayesinde hasarlı doku tamirinin de sağlanabileceği öngörülmektedir. Ancak klinik uygulamalardaki tedavinin başarısı ve hasta güvenliği için yapılacak detaylı çalışmalarla COVID-19 tedavisi için MKH uygulamalarının standardize edilmesi gerekmektedir. Bu derlemede temel olarak COVID-19 tedavisi için yapılan MKH uygulamaları incelenmiştir. Metin içerisinde sırasıyla SARS-CoV-2 ve COVID-19 hastalığı kısaca açıklandıktan sonra yapılan aşı çalışmaları ile enfeksiyon sonrası uygulanan terapiler özetlenerek COVID-19 tedavisi için geliştirilen ve umut vaat eden MKH uygulamaları, MKH'lerin tedavideki rolü, hareket mekanizması, uygulama güvenliği ve etik konusu tartışılmıştır.Öğe Decellularization of bovine spinal cord meninges via supercritical CO2 and evaluating the extracellular matrix performance for neural tissue engineering applications(Mary Ann Liebert, Inc, 2024) Kurt, Tuğçe; Özüdoğru, Eren; Cengiz, Uğur; Derkuş, B.; Arslan, Yavuz Emre[No abstract available]Öğe Decellularized Bone Extracellular Matrix-Coated Electrospun PBAT Microfibrous Membranes with Cell Instructive Ability and Improved Bone Tissue Forming Capacity(Wiley-V C H Verlag Gmbh, 2022) Karakaya, Ece; Erdogan, Yasar Kemal; Arslan, Tugba Sezgin; Arslan, Yavuz Emre; Odabas, Sedat; Ercan, Batur; Emregul, EmelCurrent approaches to develop bone tissue engineering scaffolds have some limitations and shortcomings. They mainly suffer from combining mechanical stability and bioactivity on the same platform. Synthetic polymers are able to produce mechanically stable sturctures with fibrous morphology when they are electrospun, however, they cannot exhibit bioactivity, which is crucial for tissue engineering and regenerative medicine. One current strategy to bring bioactivity in synthetic materials is to combine extracellular matrix (ECM)-sourced materials with biologically inert synthetic materials. ECM-sourced materials without any modifications are mechanically unstable; therefore, reinforcing them with mechanically stable platforms is indispensable. In order to overcome this bifacial problem, we have demonstrated that poly(butylene adipate-co-terephthalate) (PBAT) electrospun microfibrous membranes can be successfully modified with decellularized bone ECM to endow fibers with bioactive hydrogel and mimic natural micro-features of the native bone tissue. The developed structures have been shown to support osteogenesis, confirmed by histochemical staining and gene expression studies. Furthermore, ECM-coated PBAT fibers, when they were aligned, supplied an improved level of osteogenesis. The strategy demonstrated can be adapted to any other tissues, and the emerging microfibrous, mechanically stable, and bioactive materials can find implications in the specific fields of tissue engineering and regenerative medicine.Öğe Decellularized spinal cord meninges extracellular matrix hydrogel that supports neurogenic differentiation and vascular structure formation(John Wiley and Sons Ltd, 2021) Özüdoğru, Eren; Işık, Melis; Eylem, Cemil Can; Nemutlu, Emirhan; Arslan, Yavuz Emre; Derkus, BurakDecellularization of extracellular matrices offers an alternative source of regenerative biomaterials that preserve biochemical structure and matrix components of native tissues. In this study, decellularized bovine spinal cord meninges (dSCM)-derived extracellular matrix hydrogel (MeninGEL) is fabricated by employing a protocol that involves physical, chemical, and enzymatic processing of spinal meninges tissue and preserves the biochemical structure of meninges. The success of decellularization is characterized by measuring the contents of residual DNA, glycosaminoglycans, and hydroxyproline, while a proteomics analysis is applied to reveal the composition of MeninGEL. Frequency and temperature sweep rheometry show that dSCM forms self-supporting hydrogel at physiological temperature. The MeninGEL possesses excellent cytocompatibility. Moreover, it is evidenced with immuno/histochemistry and gene expression studies that the hydrogel induces growth-factor free differentiation of human mesenchymal stem cells into neural-lineage cells. Furthermore, MeninGEL instructs human umbilical vein endothelial cells to form vascular branching. With its innate bioactivity and low batch-to-batch variation property, the MeninGEL has the potential to be an off-the-shelf product in nerve tissue regeneration and restoration.Öğe Decellularized tumor matrices as biomimetic cancer niche: a new perspective on cancer research and therapy(Iop Publishing Ltd, 2025) Ozudogru, Eren; Kurt, Tugce; Arslan, Yavuz EmreCancer is among the major causes of mortality, responsible for approximately 15% of all deaths worldwide. Despite remarkable progress in modern medicine, it remains a significant global health challenge. Nevertheless, conventional therapies such as chemotherapy and radiotherapy target healthy and malignant tissues, leading to adverse side effects, including hair loss, fatigue, and nausea, which significantly reduce patients' quality of life. Even more critically, the therapeutic response varies from patient to patient, which reduces the effectiveness of treatment. Therefore, cancer tissue engineering has evolved as a novel interdisciplinary field, aiming to develop structures that mimic the tumor microenvironment to elucidate cancer development mechanisms and devise effective treatment methods. However, producing a fully synthetic biosimilar matrix by assembling all individual ECM components remains unfeasible due to the heterogeneity and complex structure of tumor tissues, as well as the necessity of highly advanced micro- and nanoengineering techniques. Consequently, decellularization techniques have recently been applied to cancer tissues to produce biomimetic tumor models. In this review, we provided a comprehensive overview of the extracellular matrix (ECM) architecture and its role in tumor progression. We also discussed the structural differences between normal and malignant tissues. We briefly reviewed decellularization techniques and analytical approaches for ECM characterization. Emphasizing the cutting-edge research, we categorized developments into three groups: decellularized tumor-derived ECM (dT-ECM), hydrogels, and bioinks. Subsequently, we critically assessed the benefits, limitations, and potential future developments of dT-ECM-based strategies. Finally, we envision that tumor tissue engineering will provide preventive treatment approaches by developing patient-specific predictive and personalized cancer models through integrating advanced biomaterials with artificial intelligence and machine learning.Öğe Detergent-free decellularization of bovine costal cartilage for chondrogenic differentiation of human adipose mesenchymal stem cells in vitro(Royal Soc Chemistry, 2016) Erten, Evren; Arslan, Tugba Sezgin; Derkus, Burak; Arslan, Yavuz EmreIn this study, we report a novel, detergent-free decellularization protocol for the preparation of intact cartilage ECM-based scaffolds (CEbS) during an effective decalcification process. On treatment with 10 mM Na(2)EDTA, the amount of calcium lost was around 55% +/- 5% (percent +/- S.D.%) (n = 3) and nearly 84% of the nuclear material was removed; however, the most effective removal was observed on treatment with 10 mM Na2EDTA combined with 0.5% Triton X-100 for 48 hours. Notably, our proposed method decreased the GAG content by only 5% compared to untreated CEbS (380.37 +/- 16.02 mu g mg(-1) dry weight). There was no significant difference in hydroxyproline content between the untreated (13.04 +/- 1.51 mg mg(-1) dry weight) sample and our proposed method (12.95 +/- 1.55 mg mg(-1) dry weight). The scaffold morphology and cell attachment were evaluated using SEM micrographs, and the cells that were inoculated with detergent-free decellularized CEbS for 14, 21 and 28 days covered the scaffold area, including the porous cavities. Microscopic observations showed that the cell density increased day by day and there was no cytotoxic evidence for the scaffolds, which is a desirable environment for cells. The histochemical and immunohistochemical assessments are supported by glycosaminoglycan and hydroxyproline assays. The proposed detergent-free decellularization technique could be a promising method for cartilage tissue regeneration.Öğe Development and characterization of skin substitutes from electrospun polycaprolactone/silk fibroin(SAGE Publications Ltd, 2024) Yıldız, Gülşah; Arslan, Yavuz Emre; Derkus, Burak; Sezgin, Billur; Menceloglu, Yusuf Ziya; Bayar, Gürkan RaşitTissue-engineered skin substitutes have great potential to treat chronic wounds and high-degree burns. Existing solutions, such as Integra Dermal Template, are extensively used for skin defects. However, these templates are still lacking in terms of recreating the functionality of the native tissue and providing scarless healing. In this study, polycaprolactone/silk fibroin (PCL/SF)-based nanofibers with varying blends were fabricated and characterized to develop a novel skin substitute. Morphological analysis showed that the nanofiber distribution of each sample was homogenous without showing any beads. In terms of mechanical properties, all the samples other than SF showed sufficient mechanical strength. It was observed that adding a specific amount of SF into the PCL nanofiber improves the tensile strength of the samples due to the introduction of intermolecular interactions from the functional groups of SF. In addition, incorporating SF into PCL improved Young’s modulus of the PCL nanofibers since SF provides stiffness and structural integrity to the overall structure. Water contact angle analysis was performed as the hydrophilicity of a biomaterial is a significant factor in cell functionality. Each sample had a contact angle between 33° and 48°, indicating the adequate hydrophilicity of nanofibers for advanced cell proliferation other than PCL. Cell proliferation and viability studies were conducted with the seeding of primary human keratinocytes on the samples. It was examined that scaffolds containing blends of PCL and SF resulted in higher cell proliferation and viability after 7 days compared to pure PCL and SF nanofibers.Öğe Development of a novel aptasensor using jellyfish collagen as matrix and thrombin detection in blood samples obtained from patients with various neurodisease(Elsevier Science Sa, 2016) Derkus, Burak; Arslan, Yavuz Emre; Bayrac, Abdullah Tahir; Kantarcioglu, Ilkim; Emregul, Kaan C.; Emregul, EmelIn the present study, we describe the isolation and detailed characterization of pepsin-soluble atelocollagen from Rhizostoma pulmo species jellyfish and application towards thrombin apta-sensing. Various analysis methods including infra-red spectroscopy, SDS-PAGE electrophoresis, and amino acid analysis have been applied for the characterization of jellyfish collagen and compared with both rat tail collagen and BSA. When comparing the two collagen types derived from jellyfish and rat tail, jellyfish collagen was observed to contain a relatively high amount of glutamic acid (61 residues/1000 residues) and alanine (63 residues/1000 residues) but low amounts of proline (113 residues/1000 residues). On the other hand, pepsin-soluble jellyfish collagen contained a small quantity of tyrosine indicating the purity of atelo-collagen. Electrochemical impedance spectroscopy is the main analyzing technique of the developed apta-sensor. The proposed apta-sensor has a detection limit of 6.25 nM thrombin. Clinical application were performed with analysis of the thrombin levels in blood and CSF samples obtained from patients with Multiple Sclerosis, Myastenia Gravis, Epilepsy, Parkinson, Polyneuropathy and healthy donors using both the apta-sensor and commercial ELISA kit. The results revealed the proposed system to be a promising candidate for clinical analysis of thrombin. (C) 2016 Elsevier B.V. All rights reserved.Öğe Development of Ductile-Sticky Bone Fillers from Biodegradable Hydrolyzed Wool-Keratin and Silk Fibroin(Wiley-V C H Verlag Gmbh, 2024) Bekar, Şerife; Sezgin Arslan, Tuğba; Arslan, Yavuz EmreIn the present study, a method is proposed for preparing novel ductile-sticky materials that can be used as bone void fillers using hydrolyzed wool-keratin (WK) and silk fibroin (SF). This methodology uses citric acid as a cross-linking agent in preparing keratin paste (KP) owing to its non-toxicity and plasticizing properties. The Keratin paste-silk fibroin structure (KPSF) is obtained by adding SF, which possesses biocompatible and superior mechanical properties. Methanol treatment is employed on the KPSF mixture to convert the Silk I structure in the SF to Silk II, resulting in a water-insoluble and tightly packed proteinaceous structure. The physicochemical properties of both bioscaffolds are investigated and discussed in detail by comparison. Based on the findings, the presence of SF in the KPSF structure contributed to properties such as flexibility and porosity. In ovo CAM analysis reveals that both materials exhibit proangiogenic properties and are biocompatible. KP and KPSF bioscaffolds can be converted into ductile-sticky forms by adding water. It believes that these forms can easily apply to bone defect areas, particularly cavitary bone defects. Furthermore, KPSF bioscaffolds, with better mechanical properties, can be considered candidates for use in non-load-bearing bone tissue engineering applications. This study introduces a strategy using citric acid as a crosslinker and plasticizer to create ductile-sticky Keratin Paste (KP) and Keratin paste-silk fibroin (KPSF) bone fillers. This study proposes that moldable ductile-sticky KP and KPSF materials are suitable for cavitary bone defects, while lyophilized forms of KPSF bioscaffolds, offering flexibility and robustness, are ideal for non-load-bearing bone tissue engineering applications. image
