E. coli O157:H7 Detection Using Surface Plasmon Resonance Based Biosensor

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dc.contributor.authorEser, Esma
dc.contributor.authorEkiz, Okan Öner
dc.contributor.authorEkiz, H. İbrahim
dc.date.accessioned2025-05-29T05:20:51Z
dc.date.available2025-05-29T05:20:51Z
dc.date.issued2023
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractThe detection of foodborne pathogenic bacteria remains a significant challenge, and the need for fast and sensitive detection methods is becoming increasingly important. Escherichia coli is a prevalent bacteria associated with foodborne illness, and this study aimed to evaluate the ability of a surface plasmon resonance (SPR) based biosensor to detect E. coli O157:H7 at low levels in pure culture and artificially contaminated bay leaves (Laurus nobilis) using different injection methods. To develop a biological sensing surface, the sensor surface was functionalized with 3-aminopropyltriethoxysilane (APTES), and polyclonal antibodies were immobilized on the surface for bacteria detection. Bacterial attachment to the antibodies resulted in a change in resonance angle. The biosensor was able to discriminate between cellular concentrations of 103 to 107 CFU/mL and showed potential in detecting different pathogens in various food samples. Before the SPR detection, the sample preparation step was optimized to ensure complex food matrices were suitable for SPR analysis. The results suggest that the SPR based biosensor is a promising tool for the rapid detection of foodborne pathogens in complex food matrices.
dc.identifier.doi10.15671/hjbc.1271685
dc.identifier.endpage372
dc.identifier.issn2687-475X
dc.identifier.issn2687-475X
dc.identifier.issue4
dc.identifier.startpage359
dc.identifier.urihttps://doi.org/10.15671/hjbc.1271685
dc.identifier.urihttps://hdl.handle.net/20.500.12428/30394
dc.identifier.volume51
dc.language.isoen
dc.publisherHacettepe University
dc.relation.ispartofHacettepe Journal of Biology and Chemistry
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_DergiPark_20250529
dc.subjectE.coli O157:H7
dc.subjectSPR based biosensors
dc.subjectBacterial detection
dc.subjectLaurus nobilis
dc.titleE. coli O157:H7 Detection Using Surface Plasmon Resonance Based Biosensor
dc.typeResearch Article

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