Phylogenetic Analysis and Extracellular Enzyme Profiles of Yeast Strains Isolated from Raspberry Fruits

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dc.contributor.authorGenç, Tülay Turgut
dc.contributor.authorGünay, Melih
dc.date.accessioned2025-01-27T19:28:49Z
dc.date.available2025-01-27T19:28:49Z
dc.date.issued2021
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractRaspberry fruit contains phenolic compounds, flavones, flavonoids, vitamins, and antioxidant substances that are important properties for health and pharmacological sciences. Edible berries provide also a suitable habitat for the growth of various microorganisms. In this study, yeast biota associated with raspberry fruits was determined by molecular identification techniques. Raspberry fruits were collected from Çanakkale, Gelibolu (Gallipoli). Yeast strains were isolated and then identified by using the analysis of ITS1-5.8S-ITS2 rDNA gene sequences. The phylogenetic analysis of all yeast strains was carried out by using the MEGA–X phylogenetic analysis tool. The extracellular enzyme profiles of identified yeast species were determined by the API-ZYM kit system. The distribution of yeast species on the raspberry fruits was determined as Hanseniaspora uvarum, Metschnikowia viticola, Aureobasidium pullulans, and Metschnikowia pulcherrima. It was observed that yeast strains belong to Metschnikowia genus were dominant on raspberry fruits. All yeast strains in Metschnikowia genus showed different enzyme profiles against seven extracellular enzymes. These enzymes may be the discriminatory enzymes for the yeast strains in the Metschnikowia genus. When the phylogenetic relationships among all yeast strains were investigated, all strains were divided into two main clades. While the first clade consists of only Metschnikowia genus, second clade includes H. uvarum and A. pullulans yeast species. Our results indicated that restriction patterns and also extracellular enzyme profiles could be utilized for differentiation of yeast strains within the genus. M. pulcherrima, H. uvarum, and A. pullulans can be used for industrial applications for future researches.
dc.identifier.doi10.28979/jarnas.890860
dc.identifier.endpage171
dc.identifier.issn2757-5195
dc.identifier.issue1
dc.identifier.startpage160
dc.identifier.trdizinid1114203
dc.identifier.urihttps://doi.org/10.28979/jarnas.890860
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1114203
dc.identifier.urihttps://hdl.handle.net/20.500.12428/15899
dc.identifier.volume7
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.relation.ispartofJournal of advanced research in natural and applied sciences (Online)
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_TRD_20250125
dc.subjectBiyoloji
dc.subjectMantar Bilimi
dc.titlePhylogenetic Analysis and Extracellular Enzyme Profiles of Yeast Strains Isolated from Raspberry Fruits
dc.typeArticle

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