Fenazopridin hidroklorür'ün poli(p-aminobenzen sülfonik asit)-modifiye camsı karbon elektrotta ilaç dozaj formlarından miktarının belirlenmesi
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Tarih
2014
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Çanakkale Onsekiz Mart Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Bu çalışmada, fenazopridin hidroklorür (FAP) adlı etken maddenin, elektrokimyasal indirgenme özelliğinden faydalanarak modifiye camsı karbon elektrotta, ticari formundan miktarı belirlendi. Optimum şartları belirlemek için farklı destek elektrolitlerde diferansiyel puls voltametrisi ve dönüşümlü voltametri ile indirgenme özelliği çalışıldı. FAP için modifiye camsı karbon elektrotta keskin pikin ve maksimum akımın gözlendiği pH=7,00 fosfat tampon çözeltisi destek elektrolit olarak seçildi. FAP'ın pik akımına ve pik potansiyeline pH' ın etkisi diferansiyel puls voltametri tekniği (DPV) ve tarama hızının pik akımına etkisi ise dönüşümlü voltametri tekniği (CV) ile incelendi. Belirme sınırı (LOD), kantitatif tayin sınırı (LOQ) ve konsantrasyon aralığı gibi analitik parametreler belirlendikten sonra ilaç tabletlerindeki FAP miktarı bulundu. Uygulanan voltametrik yöntemin, doğruluk ve kesinliğini kontrol etmek amacı ile ilaç tabletinden FAP'ın geri kazanım çalışmaları yapıldı. Ayrıca FAP'ın modifiye camsı karbon elektrotta indirgenme mekanizması da önerildi. Anahtar Kelimeler: Fenazopridin Hidroklorür, Voltametrik Yöntem, Diferansiyel Puls Voltametrisi (DPV), Dönüşümlü Voltametri (CV), Modifiye Camsı Karbon Elektrot, Ticari İlaç Tabletleri.
In this study, active compounds named phenazopyridine hydrocloride (FAP) was determined from commercial drug form based on electrochemical reduction properties at modified glassy carbon electrode. In different supporting electrolyte, reduction was studied by voltammetric methods to investigate the optimum conditions. pH=7,00 Phosphate solution in which sharp peak and maxium current was observed, at modified glassy carbon electrode for FAP, was selected as supporting electrolyte. The effects of pH on the peak current and peak potential for material was investigated by differential pulse voltammetry (DPV) and the effect of scan rate on the peak current was investigated by cyclic voltammetry (CV) techniques. After analytical parameters, such as limit of detection (LOD), limit of quantitation (LOQ) and range of concentration were determined, the amount of FAP was determined from drug tablets. In order to check accucarcy and precision of applied voltammetric method, recovery experiment of FAP was carried out from the drug tablet. Also, reduction mechanism of FAP at modified glassy carbon elektrode was proposed. Keywords: Phenazopyridine Hydrocloride, Voltammetric Method, Differential Pulse Voltammetry (DPV), Cyclic Voltammetry (CV), Modified Glassy Carbon Electrode, Commercial Drug Tablets.
In this study, active compounds named phenazopyridine hydrocloride (FAP) was determined from commercial drug form based on electrochemical reduction properties at modified glassy carbon electrode. In different supporting electrolyte, reduction was studied by voltammetric methods to investigate the optimum conditions. pH=7,00 Phosphate solution in which sharp peak and maxium current was observed, at modified glassy carbon electrode for FAP, was selected as supporting electrolyte. The effects of pH on the peak current and peak potential for material was investigated by differential pulse voltammetry (DPV) and the effect of scan rate on the peak current was investigated by cyclic voltammetry (CV) techniques. After analytical parameters, such as limit of detection (LOD), limit of quantitation (LOQ) and range of concentration were determined, the amount of FAP was determined from drug tablets. In order to check accucarcy and precision of applied voltammetric method, recovery experiment of FAP was carried out from the drug tablet. Also, reduction mechanism of FAP at modified glassy carbon elektrode was proposed. Keywords: Phenazopyridine Hydrocloride, Voltammetric Method, Differential Pulse Voltammetry (DPV), Cyclic Voltammetry (CV), Modified Glassy Carbon Electrode, Commercial Drug Tablets.
Açıklama
Fen Bilimleri Enstitüsü, Kimya Ana Bilim Dalı
Anahtar Kelimeler
Kimya, Chemistry
