Acrylamide-Sepiolite Based Composite Hydrogels for Immobilization of Invertase

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dc.authoridSaraydin, Dursun/0000-0001-7191-510X
dc.contributor.authorOztop, H. Nursevin
dc.contributor.authorHepokur, Ceylan
dc.contributor.authorSaraydin, Dursun
dc.date.accessioned2025-01-27T20:34:34Z
dc.date.available2025-01-27T20:34:34Z
dc.date.issued2009
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractNovel composite hydrogels, poly(acrylamide)-sepiolite (PAS), poly(acrylamide/acrylic acid)-sepiolite (PAAS), and poly(acrylamide/itaconic acid)-sepiolite (PAIS) were prepared and used for the immobilization of invertase. The parameters of equilibrium swelling, diffusional exponent, and diffusion coefficient of these hydrogels were calculated from swelling experiments. Invertase was immobilized onto PAS, PAAS, and PAIS and immobilized invertases (PASI, PAASI, and PAISI) were prepared. Optimum pH values for free invertase, PASI, PAASI, and PAISI are found to be 5, 5.5, 4.5, and 6, respectively, and the optimum temperatures were 30, 50, 50, and 35 degrees C for free invertase PASI, PAASI, and PAISI. It was found that K-m values of free invertase, PASI, PAASI, and PAISI were 11.3, 41.0, 94.5, and 56.0 mM, respectively. V-max values were 2 mu mol/min for free invertase, 8.10 mu mol/min for PASI, 1.30 mu mol/min for PAASI, and 0.42 mu mol/min for PAISI, respectively. The invertase immobilized hydrogels showed excellent, temperature, storage, and operational stability.
dc.description.sponsorshipCumhuriyet Univ. Research Fund
dc.description.sponsorshipThe authors gratefully acknowledge the financial support provided by the Cumhuriyet Univ. Research Fund through Project F-203.
dc.identifier.doi10.1111/j.1750-3841.2009.01302.x
dc.identifier.endpageN49
dc.identifier.issn0022-1147
dc.identifier.issn1750-3841
dc.identifier.issue7
dc.identifier.pmid19895491
dc.identifier.startpageN45
dc.identifier.urihttps://doi.org/10.1111/j.1750-3841.2009.01302.x
dc.identifier.urihttps://hdl.handle.net/20.500.12428/23359
dc.identifier.volume74
dc.identifier.wosWOS:000269498300038
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherWiley
dc.relation.ispartofJournal of Food Science
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectacrylamide
dc.subjectcomposite
dc.subjectimmobilization
dc.subjectinvertase
dc.subjectsepiolite
dc.titleAcrylamide-Sepiolite Based Composite Hydrogels for Immobilization of Invertase
dc.typeArticle

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