Evaluation of tuberculosis laboratory results in çanakkale onsekiz mart university research and education hospital for 2009-2011
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Tarih
2012
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Objective: Tuberculosis microbiological laboratory diagnosis was firstly started in year 2009, in Microbiology Laboratory of Onsekiz Mart University Research and Education Hospital in Çanakkale. We aimed at this study to present our laboratory data and to evaluate the methods which were used for the diagnosis of micobacteria. Method: Samples sent to our laboratory for tuberculosis culture were stained by Ehrlich-Ziehl-Neelsen (EZN) method and evaluated microscopically. After processing of samples, each sample was inoculated to Löwenstein-Jensen medium (LJ) and BACTEC MGIT 960 (Mycobacteria Growth Indicator Tube, Becton Dickinson, USA) liquid based medium. If suspected growth was detected, Mycobacterium tuberculosis complex (MTBC) typing was made and if requested antituberculosis drug susceptibility for streptomycin (STR), isoniazid (INH), rifampicin (RF) and ethambutol (ETM) tested. Samples from normally sterile body sites cultured directly, others were firstly decontaminated and concentrated. Results: During the study period 1.048 samples from 667 patient has been processed. Seventy eight samples (7.44%) from 54 patients were found positive by BACTEC MGIT system: 71 of them MTBC and seven of them were mycobacteria other than tuberculosis (MOTT). By LJ medium 64 MTBC and 4 MOTT strain, totally 68 mycobacterium were isolated. Mean time for detecting positive culture by MGIT 960 was 11.8 days (± 7.45 SD). With EZN stain, 49 samples were detected as acido resistant bacilli and only 42 (86%) of them were positive by culture. Antituberculosis drug susceptibilty was evaluated at isolates of 25 from 54 patients. A resistance to at least one of the drugs were detected in six isolates. It is found that five isolates were resistant to STR, three were resistant to INH and one was resistant to ETM. Three isolates were resistant to both STR and INH. Rifampicin resistance was not detected in MTBC. Conclusion: With this study we presented first tuberculosis laboratory findings from our province, Çanakkale. Tuberculosis microbiological culture and antituberculosis susceptibility tests can be made using Bactec MGIT 960 system which is easier and faster than solid media culture. In tuberculosis diagnosis sensitivity of culture is higher than microscopical evaluation. It was concluded that although microscopic examination has low sensitivity, for early detection of tuberculosis both culture and staining should be used together for routine detection of tuberculosis.
Açıklama
Anahtar Kelimeler
Mycobacterium tuberculosis, Culture, Antituberculosis susceptibility
Kaynak
Turk Hijyen ve Deneysel Biyoloji Dergisi
WoS Q Değeri
Scopus Q Değeri
Q4
Cilt
69
Sayı
3
